| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Fertilization begins with species-restricted interaction of sperm and the egg coat: an extracellular meshwork of filaments constituted by glycoproteins (called ZP protein). Dicalcin, a novel ZP protein-binding protein in Xenopus egg coat, suppresses the fertilization rate through its binding to gp41. We analyzed nanoscale-structural properties of the egg coat using transmission electron microscopy, following clamp of the egg coat under either excess of dicalcin's action (low fertilization competence) or lack of the action (high competence), and discussed concerning the mechanism for the dynamic remodeling of the architecture of the egg coat. Additionally, we identified the amino acid region responsible for the action of mouse dicalcin using synthesized partial peptides based upon the sequence of dicalcin. These results indicated that dicalcin affects fertilization efficiency through regulating fertilization competence of the egg coat in frogs and mice.
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