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Effect of glucagon on antidiabetic effect induced by nitrite

Research Project

Project/Area Number 16K08550
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General pharmacology
Research InstitutionThe University of Tokushima

Principal Investigator

TSUCHIYA Koichiro  徳島大学, 大学院医歯薬学研究部(薬学域), 教授 (70301314)

Co-Investigator(Kenkyū-buntansha) 石澤 有紀  徳島大学, AWAサポートセンター, 准教授 (40610192)
池田 康将  徳島大学, 大学院医歯薬学研究部(医学域), 准教授 (60432754)
宮本 理人  徳島大学, 大学院医歯薬学研究部(薬学域), 助教 (60456887)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords亜硝酸イオン / 硝酸イオン / グルカゴン / 一酸化窒素 / 亜硝酸塩 / 硝酸塩 / αTC細胞 / AMPK / 糖尿病 / 亜硝酸 / 生理活性物質
Outline of Final Research Achievements

In this study, we examined the effect of nitrite on glucagon secretion using αTC cells. When nitrite was added to αTC cells, a concentration-dependent decrease in glucagon secretion was observed. Furthermore, nitrite-derived suppression of glucagon and phosphorylation of AMPK were amerioated in the presence of the AMPK inhibitors compound C.
Moreover, gluconeogenesis from liver cells was suppressed by nitrite through AMPK activation, suggesting that nitrite has the potential to improve fasting blood glucose.

Academic Significance and Societal Importance of the Research Achievements

我々は硝酸塩を食物から摂取しており、その一部は口腔内細菌によって亜硝酸イオンに還元され、さらにNOへと変換されている。実験動物に亜硝酸イオンを経口投与すると空腹時血糖値の有意な低下が観察されたことからグルカゴン分泌に対する亜硝酸イオンの影響を検討したところ、亜硝酸イオンはAMPK活性化を介してグルカゴン分泌および肝細胞からの糖新生に影響を与えていることが示唆され、亜硝酸イオンの新たな生理作用を見出した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Nitrite Activates 5′AMP-Activated Protein Kinase-Endothelial Nitric Oxide Synthase Pathway in Human Glomerular Endothelial Cells2017

    • Author(s)
      Miyamoto Licht、Yamane Megumi、Tomida Yosuke、Kono Mai、Yamaoka Tomomi、Kawasaki Aya、Hatano Aya、Tsuda Katsunori、Xu Wenting、Ikeda Yasumasa、Tamaki Toshiaki、Tsuchiya Koichiro
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 40 Issue: 11 Pages: 1866-1872

    • DOI

      10.1248/bpb.b17-00316

    • NAID

      130006191905

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] α-TCのグルカゴン分泌におけるSGLT2阻害薬の影響2018

    • Author(s)
      中山卓、宮本理人、服部真奈、井上陽加、土屋浩一郎
    • Organizer
      第57回 中国四国支部学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 亜硝酸塩による脂肪組織への抗肥満的な影響2018

    • Author(s)
      井上陽加、宮本理人、服部真奈、池田康将、玉置俊晃、 土屋浩一郎
    • Organizer
      第134回日本薬理学会近畿部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Effect of SGLT2 inhibitors on glucagon secretion in pancreatic α cells2018

    • Author(s)
      Suguru Nakayama, Licht Miyamoto, Koichiro Tsuchiya
    • Organizer
      18th World congress of basic and clinical pharmacology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nitrite exerts its physiological effects through NO-dependent and -independent manner2018

    • Author(s)
      Koichiro Tsuchiya
    • Organizer
      10th Meeting of the Canadian Oxidative Stress Consortium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 食餌性亜硝酸塩の新たな薬理学的作用2017

    • Author(s)
      土屋浩一郎
    • Organizer
      第17回日本NO学会学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 食餌性硝酸・亜硝酸塩の生理的役割について2017

    • Author(s)
      土屋浩一郎
    • Organizer
      第53回全肥商連全国研修会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 肥料、ミネラルとヒトの健康、硝酸塩の臓器保護作用2017

    • Author(s)
      土屋浩一郎
    • Organizer
      日本土壌肥料学会2017年度大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Nitrite activates 5’AMP-Activated Protein Kinase -;endothelial nitric oxide synthase pathway in human glomerular endothelial cells.2016

    • Author(s)
      Koichiro Tsuchiya, Licht Miyamoto, Yosuke Tomida, Megumi Yamane, Yuuki Tsuchihashi, Katsunori Tsuda, Yasumasa Ikeda, Toshiaki Tamaki
    • Organizer
      第16回日本NO学会学術集会&第9回国際NO学会
    • Place of Presentation
      仙台国際センター(宮城県・仙台市)
    • Year and Date
      2016-05-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Dietary nitrite and renal function2016

    • Author(s)
      Koichiro Tsuchiya
    • Organizer
      韓国東國大学校薬学部講演会
    • Place of Presentation
      Gyeonggi-do (Korea)
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] 肥料の夜明け2018

    • Author(s)
      渡辺和彦 編著, 土屋浩一郎(分担執筆)
    • Total Pages
      223
    • Publisher
      化学工業日報社(東京)
    • ISBN
      9784873267036
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2021-01-27  

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