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The transport mechanism of the lactate transporter between Sertoli cells and spermatocytes and the role of seminolipid in it.

Research Project

Project/Area Number 16K08591
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General medical chemistry
Research InstitutionKochi University

Principal Investigator

Honke Koichi  高知大学, その他部局等, 副学長 (80190263)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords精子形成 / 精母細胞 / セルトリ細胞 / セミノリピド / 乳酸 / MCT4 / ベイシジン / 細胞外小胞 / 乳酸トランスポーター / 発生・分化 / 生体分子 / 糖鎖 / 脂質
Outline of Final Research Achievements

We have elucidated the molecular function of seminolipid, which is the sulfated glycolipid specifically expressed in mammalian testes. Since the testis has the blood-testis barrier, spermatocytes utilize lactate that is produced from glucose in Sertoli cells as the energy source. We found that the lactate transporter on the plasm membrane of spermatocytes is MCT4 and it can function only after it forms assembly with basigin. After the MCT4 gene is transcribed and translated in Sertoli cells, MCT4 proteins are found to be secreted into the extracellular vesicles and transported to spermatocytes. We found that this secretion of the extracellular vesicles from Sertoli cells is stimulated by seminolipid.

Academic Significance and Societal Importance of the Research Achievements

男性不妊の多くは精子形成障害が原因である。哺乳動物の精子形成は精巣中で起こるが、精子形成細胞と支持細胞のセルトリ細胞の相互作用で進行する。精子形成細胞は2回減数分裂を行い、半数体の精子になる。セミノリピドを欠損したマウスは、第一減数分裂の途中で停止し不妊となることから、精子形成に必須の分子であることがわかっているが、その分子メカニズムは不明であった。本研究によってその分子メカニズムが明らかとなった。本研究により、セルトリ細胞と精子形成細胞間のコミュニケーションに細胞外小胞が使われていることが明らかとなった。正常の発生過程においても細胞外小胞の重要性が明確となった。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 5 results) Book (1 results)

  • [Journal Article] Analysis of lipid raft molecules in the living brain slices2018

    • Author(s)
      Kotani N, Nakano T, Ida Y, Ito R, Hashizume M, Yamaguchi A, Seo M, Araki T, Hojo Y, Honke K, Murakoshi T
    • Journal Title

      Neurochem Int

      Volume: 119 Pages: 140-150

    • DOI

      10.1016/j.neuint.2017.08.012

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ASC amino acid transporter 2, defined by enzyme-mediated activation of radical sources, enhances malignancy of GD2-positive small-cell lung cancer.2018

    • Author(s)
      Nobutoshi Esaki, Yuki Ohkawa, Noboru Hashimoto, Yuhsuke Tsuda, Yuhsuke Ohmi, Robiul H. Bhuiyan, Norihiro Kotani, Koichi Honke, Atsushi Enomoto, Masahide Takahashi, Keiko Furukawa, Koichi Furukawa.
    • Journal Title

      Cancer sci.

      Volume: 109 Issue: 1 Pages: 141-153

    • DOI

      10.1111/cas.13448

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biological functions of sulfoglycolipids and the EMARS method for identification of co-clustered molecules in the membrane microdomains.2018

    • Author(s)
      Honke K.
    • Journal Title

      J. Biochem.

      Volume: 163 Issue: 4 Pages: 253-263

    • DOI

      10.1093/jb/mvx078

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 1.Imamaki R. Ogawa K, Kizuka Y, Komi Y, Kojima S, Kotani N, Honke K, Honda T, Taniguchi N, and Kitazume S2018

    • Author(s)
      1.Imamaki R. Ogawa K, Kizuka Y, Komi Y, Kojima S, Kotani N, Honke K, Honda T, Taniguchi N, and Kitazume S
    • Journal Title

      Oncogene

      Volume: - Issue: 31 Pages: 4287-4299

    • DOI

      10.1038/s41388-018-0271-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tumor-dependent secretion of close homolog of L1 results in elevation of its circulating level in mouse model for human lung tumor2018

    • Author(s)
      Kotani Norihiro、Ida Yui、Nakano Takanari、Sato Izumi、Kuwahara Ryusuke、Yamaguchi Arisa、Tomita Masahiro、Honke Koichi、Murakoshi Takayuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 501 Issue: 4 Pages: 982-987

    • DOI

      10.1016/j.bbrc.2018.05.096

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sulfatide species with various fatty acid chains in oligodendrocytes at different developmental stages determined by imaging mass spectrometry2017

    • Author(s)
      Yukie Hirahara, Taketoshi Wakabayashi, Tetsuji Mori, Taro Koike, Ikuko Yao, Masayuki Tsuda , Koichi Honke, Hitoshi Gotoh, Katsuhiko Ono, Hisao Yamada.
    • Journal Title

      Journal of Neurochemistry

      Volume: 140 Issue: 3 Pages: 435-450

    • DOI

      10.1111/jnc.13897

    • NAID

      120006778373

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ganglioside GD3 enhances invasiveness via Yes activation by forming a complex of GD3/PDGFRα/Yes in gliomas2015

    • Author(s)
      Yuki Ohkawa, Hiroyuki Momota, Akira Kato, Noboru Hashimoto, Yuhsuke Tsuda, Norihiro Kotani, Koichi Honke, Akio Suzumura, Keiko Furukawa, Yuhsuke Ohmi, Atsushi Natsume, Toshihiko Wakabayashi and *Furukawa K
    • Journal Title

      J. Biol. Chem.

      Volume: 290 Issue: 32 Pages: 16043-16058

    • DOI

      10.1074/jbc.m115.708834

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] ユニークな脂肪酸組成をもつリン脂質分子種がつくる神経細胞膜機能ドメイン2018

    • Author(s)
      本家孝一
    • Organizer
      2018年生理学研究所研究会「オルガネラ膜ドメインの機能と病態」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 膜マイクロドメインを視る分子の眼2018

    • Author(s)
      本家孝一
    • Organizer
      第31回植物脂質シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] EMARS法の膜マイクロドメイン研究への応用2018

    • Author(s)
      本家孝一
    • Organizer
      第123回日本解剖学会全国学術集会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Seminolipidはセルトリ細胞のexosome分泌を促進して精子形成細胞へ乳酸トランスポーターMCT4を送達する。2016

    • Author(s)
      山下竜幸、仁尾景子、矢生健一、宮原 馨、津田雅之、小杉智規、門松健治、本家孝一
    • Organizer
      第89回日本生化学会大会、シンポジウム
    • Place of Presentation
      仙台国際センター/東北大学川内北キャンパス(宮城県仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 硫酸化糖脂質セミノリピドはセルトリ細胞のエクソソーム分泌を促進する。2016

    • Author(s)
      山下竜幸、仁尾景子、矢生健一、宮原 馨、津田雅之、小杉智規、門松健治、本家孝一
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと(高知県高知市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Research-status Report
  • [Presentation] A sulfated glycolipid, seminolipid and basigin are requied for the functional expression of the lactate transporter in spermatocytes.2016

    • Author(s)
      Honke K, Yamashita T, Kosugi T, Kadomatsu K.
    • Organizer
      NOW-JSPS 2016 Symposium “Glycobiology in Health and Disease”
    • Place of Presentation
      Leiden, Netherland
    • Year and Date
      2016-04-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Medical Biochemistry, 5th Ed.2019

    • Author(s)
      Elbe AD, Honke K.
    • Total Pages
      27
    • Publisher
      Elsevier
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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