Analysis of plasticity of cancer-initiating cells in malignant tumors
Project/Area Number |
16K08649
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
|
Research Institution | Chiba University (2018) Osaka University (2016-2017) |
Principal Investigator |
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 腫瘍幹細胞 / 病理学 / 子宮内膜癌 / アルデヒド脱水素酵素 / 可塑性 / 癌 |
Outline of Final Research Achievements |
RNA-seq analysis was performed on cells in a state in which cancer-initiating cells (CICs) were likely to cause plasticity and in a state in which CICs were not easily induced using endometrioid carcinoma cell lines. Glycoprotein M6B (GPM6B) found in a state in which plasticity was likely to occur was immunohistochemically expressed in agreement with the ALDH-positive area in endometrioid carcinoma cases. When sarcosine, which was produced from glycine by glycine N-methyltransferase, which was expressed in a state that was difficult to induce CICs, was administered to cancer cell lines, the proportion of cells with high ALDH activity was decreased in a concentration-dependent manner. It was suggested that these factors might be involved in the control of CIC plasticity.
|
Academic Significance and Societal Importance of the Research Achievements |
腫瘍の中には化学療法や放射線療法に抵抗性で,再発や転移の原因になるとされる「腫瘍幹細胞」と呼ばれる細胞群が存在し,「腫瘍幹細胞」の制御ががん治療の重要な鍵となっている.従来,「腫瘍幹細胞」から「腫瘍幹細胞」と「非腫瘍幹細胞」が生み出され,「非腫瘍幹細胞」から「腫瘍幹細胞」にはならないとされてきたが,「非腫瘍幹細胞」が「腫瘍幹細胞」に転換しうる「可塑性」を有することが報告され,治療を考える上でより複雑な状況になってきている.この可塑性のメカニズムを解明することでがん治療への応用につながる可能性があり,本研究成果は学術的にも社会的にも意義あるものと考える.
|
Report
(4 results)
Research Products
(75 results)
-
-
[Journal Article] Systematic diagnostics of the electrical, optical, and physicochemical characteristics of low-temperature atmospheric-pressure helium plasma sources.2019
Author(s)
Takeda K, Yamada H, Ishikawa K, Sakakita H, Kim J, Ueda M, Ikeda JI, Akimoto Y, Kataoka Y, Yokoyama N, Ikehara Y, Hori M
-
Journal Title
Journal of Physics D: Applied Physics
Volume: -
Related Report
Peer Reviewed
-
[Journal Article] Predictive value of MLH1 and PD-L1 expression for prognosis and response to preoperative chemotherapy in gastric cancer.2019
Author(s)
Hashimoto T, Kurokawa Y, Takahashi T, Miyazaki Y, Tanaka K, Makino T, Yamasaki M, Nakajima K, Ikeda JI, Mori M, Doki Y
-
Journal Title
Gastric Cancer
Volume: -
Issue: 4
Pages: 785-792
DOI
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Effect of glutamine on lymphoplasmacytic lymphoma, especially on the viewpoint of the differentiation into vulnerable subpopulation.2018
Author(s)
Wada N, Nojima S, Tahara SI, Ohshima K, Kurashige M, Kawasaki K, Tone M, Kusumoto S, Ikeda JI, Morii E.
-
Journal Title
Pathol Res Pract
Volume: 214
Issue: 10
Pages: 1667-1674
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Origin of cancer-associated fibroblasts and tumor-associated macrophages in humans after sex-mismatched bone marrow transplantation.2018
Author(s)
Kurashige M, Kohara M, Ohshima K, Tahara S, Hori Y, Nojima S, Wada N, Ikeda JI, Miyamura K, Ito M, Morii E.
-
Journal Title
Commun Biol.
Volume: 1
Issue: 1
Pages: 131-131
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
[Journal Article] CUBIC pathology: three-dimensional imaging for pathological diagnosis2017
Author(s)
Nojima Satoshi、Susaki Etsuo A.、Yoshida Kyotaro、Takemoto Hiroyoshi、Tsujimura Naoto、Iijima Shohei、Takachi Ko、Nakahara Yujiro、Tahara Shinichiro、Ohshima Kenji、Kurashige Masako、Hori Yumiko、Wada Naoki、Ikeda Jun-ichiro、Kumanogoh Atsushi、Morii Eiichi、Ueda Hiroki R.
-
Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 9269-9269
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Argininosuccinate Synthase 1-Deficiency Enhances the Cell Sensitivity to Arginine through Decreased DEPTOR Expression in Endometrial Cancer.2017
Author(s)
Ohshima K, Nojima S, Tahara S, Kurashige M, Hori Y, Hagiwara K, Okuzaki D, Oki S, Wada N, Ikeda JI, Kanai Y, Morii E.
-
Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 45504-45504
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
[Journal Article] Comprehensive phenotypic analysis of knockout mice deficient in cyclin G1 and cyclin G2.2016
Author(s)
Ohno S, Ikeda JI, Naito Y, Okuzaki D, Sasakura T, Fukushima K, Nishikawa Y, Ota K, Kato Y, Wang M, Torigata K, Kasama T, Uchihashi T, Miura D, Yabuta N, Morii E, Nojima H.
-
Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 39091-39091
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] MECHANISUM OF BLOOD COAGULATION INDUCED BY LOW TEMPERATURE PLASMA TREATMENT2017
Author(s)
Y. Ikehara, S. Ikehara, Y. Akimoto, H. Sakakita, K. Ishikawa, M. Ueda, J. Ikeda, J. Kim, T. Yamaguchi, H. Nakanishi, T. Shimizu, N. Shimizu, M. Hori
Organizer
International Conference on Plasma Medical Science Innovation 2017(ICPMSI2017)
Place of Presentation
名古屋大学
Year and Date
2017-02-27
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Swyer症候群の1例2016
Author(s)
城光寺龍,辻本正彦,池田純一郎,森井英一
Organizer
第74回日本病理学会近畿支部学術集会
Place of Presentation
関西医科大学
Year and Date
2016-09-17
Related Report
-
-
[Presentation] Mechanism of blood coagulation induced by plasma treatment in addition to the acceleration of the natural blood coagulation process2016
Author(s)
Y. Ikehara, S. Ikehara, Y. Akimoto, H. Sakakita, K. Ishikawa, M. Ueda, J. Ikeda, J. Kim, T. Yamaguchi, H. Nakanishi, T. Shimizu, N. Shimizu, M. Hori
Organizer
6th International Conference on Plasma Medicine (ICPM-6)
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-