| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Some cases of glomerulonephritis pose problems in accurate typing of glomerulonephritis due to lack of approproate tool for dissecting molecules responsible for glomerular lesions. We thus utlized technique of laser microdissection (LMD) and tandem mass spectrometry-based proteomic analysis (LC-MS/MS)as a sensitive and specific tool for the diagnosis of glomerulonephriatis. Using kidney specimens, we successfully diagnose accurately the type of amyloid deposited in the glomeruli while immunofluorescence or immunohistochemistry failed to reveal amyloid precursors. Especially, our system was very useful for amyloid cases diagnosed solely at autopsy because serum or urine samples of autopsy cases were generally unavailable for further testing of precursors such as free light chains. In addition, we successfully determined non-amyloid depositions such as apoE and fibrinogen using renal biopsy specimens with LMD and LC-MS/MS.
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