Functional analysis and clinical significance of EMU1 newly identified as a candidate of metastasis-promoting molecule
Project/Area Number |
16K08727
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Experimental pathology
|
Research Institution | Shizuoka Cancer Center Research Institute |
Principal Investigator |
Sugino Takashi 静岡県立静岡がんセンター(研究所), その他部局等, 研究員 (90171165)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 癌転移 / 分子機能 / 細胞接着 / 細胞増殖 / 発現分布 / がん転移 / matricellular protein / EMU1 / 接着阻害 / マウス転移モデル / 癌 / 遺伝子 |
Outline of Final Research Achievements |
Our previous analysis using mouse metastasis model identified EMU1 as a potential candidate of the metastasis-promoting genes. We sought to clarify the molecular function of EMU1 and the protein expression in the human tissues. Overexpression and knockdown studies demonstrated two novel molecular functions of EMU1; inhibition of cell-matrix adhesion and promotion of cell growth, whereas the high-expressor did not exhibited enhanced metastatic activity. Expression analysis revealed exclusive expression in the chief cells of the stomach and the beta-cells of the pancreatic islet in normal tissues. In cancers, EMU1 was expressed in breast and colon cancers with high incidence. Because of its limited expression in the normal tissues, cell-specific functions of this molecule are presumed. In addition, increased expression in human cancer cases implies that EMU1 may be a new molecular target in cancer therapy.
|
Academic Significance and Societal Importance of the Research Achievements |
EMU1は2004年にクローニングされて以来論文がなく、分子機能や疾患との関わりは不明であった。本研究ではEMU1が多機能性分子であり、細胞外では細胞-基質間の接着を阻害し、細胞内では増殖を促進することが明らかとなった。また、EMU1が特定の細胞にのみ発現することから、何らかの生理機能に関わると考えられる。さらに、癌に発現頻度が高いことから、癌治療の分子標的になり得ることが期待される。
|
Report
(4 results)
Research Products
(23 results)
-
-
-
-
-
[Journal Article] CYP3A4 Gene Is a Novel Biomarker for Predicting a Poor Prognosis in Hepatocellular Carcinoma2017
Author(s)
Ashida R, Okamura Y, Ohshima K, Kakuda Y, Uesaka K, Sugiura T, Ito T, Yamamoto Y, Sugino T, Urakami K, Kusuhara M, Yamaguchi K.
-
Journal Title
Cancer Genomics & Proteomics
Volume: 14
Issue: 6
Pages: 445-453
DOI
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] Melanoma patient response to nivolumab treatment for metastatic lung lesions: Multi-OMICS analysis in Project HOPE.2017
Author(s)
Kiyohara Y, Yoshikawa S, Otsuka M, Kondou R, Iizuka A, Nonomura C, Ohshima K, Urakami K, Kusuhara M, Nagashima T, Sugino T, Yamaguchi K, Akiyama Y.
-
Journal Title
J Dermatol
Volume: 印刷中
Issue: 10
Pages: 1156-1159
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Multi-omics Profiling of Patients with Melanoma Treated with Nivolumab in Project HOPE2017
Author(s)
Yoshikawa S, Kiyohara Y, Otsuka M, Kondou R, Nonomura C, Miyata H, Iizuka A, Ohshima K, Urakami K, Nagashima T, Kusuhara M, Sugino T, Mochizuki T, Yamaguchi K, Akiyama Y.
-
Journal Title
Anticancer Research
Volume: 37
Issue: 3
Pages: 1321-1328
DOI
Related Report
Peer Reviewed
-
[Journal Article] Combination of a STAT3 Inhibitor and an mTOR Inhibitor Against a Temozolomide-resistant Glioblastoma Cell Line2017
Author(s)
Miyata H, Ashizawa T, Iizuka A, Kondou R, Nonomura C, Sugino T, Urakami K, Asai A, Hayashi N, Mitsuya K, Nakasu Y, Yamaguchi K, Akiyama Y.
-
Journal Title
Cancer Genomics Proteomics
Volume: 14
Issue: 1
Pages: 83-91
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Multi-omics Profiling of Patients with Melanoma Treated with Nivolumab in Project HOPE.2017
Author(s)
Yoshikawa S, Kiyohara Y, Otsuka M, Kondou R, Nonomura C, Miyata H, Iizuka A, Ohshima K, Urakami K, Nagashima T, Kusuhara M, Sugino T, Mochizuki T, Yamaguchi K, Akiyama Y
-
Journal Title
Anticancer Res
Volume: 37 (3)
Pages: 1321-1328
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] <b>CD44 variant 9 expression as a predictor for gastric cancer recurrence: immunohistochemical and metabolomic analysis of surgically resected </b><b>tissues </b>2017
Author(s)
Yamakawa Y, Kusuhara M, Terashima M, Kinugasa Y, Sugino T, Abe M, Mochizuki T, Hatakeyama K, Kami K, Yamaguchi K.
-
Journal Title
Biomedical Research
Volume: 38
Issue: 1
Pages: 41-52
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Combination of a STAT3 Inhibitor and an mTOR Inhibitor Against a Temozolomide-resistant Glioblastoma Cell Line.2017
Author(s)
Miyata H, Ashizawa T, Iizuka A, Kondou R, Nonomura C, Sugino T, Urakami K, Asai A, Hayashi N, Mitsuya K, Nakasu Y, Yamaguchi K, Akiyama Y.
-
Journal Title
Cancer Genomics Proteomics
Volume: 14 (1)
Pages: 83-91
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Antitumor Effect of Programmed Death-1 (PD-1) Blockade in Humanized the NOG-MHC Double Knockout Mouse.2017
Author(s)
Ashizawa T, Iizuka A, Nonomura C, Kondou R, Maeda C, Miyata H, Sugino T, Mitsuya K, Hayashi N, Nakasu Y, Maruyama K, Yamaguchi K, Katano I, Ito M, Akiyama Y.
-
Journal Title
Clinical Cancer Research
Volume: 23(1)
Issue: 1
Pages: 149-158
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] <b>Prevalence of low-penetrant germline </b><i><b>TP53</b></i><b> D49H mutation in Japanese cancer </b><b>patients </b>2016
Author(s)
Yamaguchi Y, Torisu H, Kira R, Ishizaki Y, Sakai Y, Sanefuji M, Ichiyama T, Oka A, Kishi T, Kimura S, Kubota M, Takanashi J, Takahashi Y, Tamai H, Natsume J, Hamano S, Hirabayashi S, Maegaki Y, Mizuguchi M, Minagawa K, Yoshikawa H, Kira J, Kusunoki S, Hara T.
-
Journal Title
Biomedical Research
Volume: 37
Issue: 4
Pages: 259-264
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] Risk factors for lymph node metastasis and long-term outcomes of patients with early gastric cancer after non-curative endoscopic submucosal dissection.2016
Author(s)
Kawata N, Kakushima N, Takizawa K, Tanaka M, Makuuchi R, Tokunaga M, Tanizawa Y, Bando E, Kawamura T, Sugino T, Kusafuka K, Shimoda T, Nakajima T, Terashima M, Ono H.
-
Journal Title
Surg Endosc
Volume: 印刷中
Issue: 4
Pages: 1607-1616
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Peritoneal dissemination in early gastric cancer: importance of the lymphatic route.2016
Author(s)
Yoshida M, Sugino T, Kusafuka K, Nakajima T, Makuuchi R, Tokunaga M, Tanizawa Y, Bando E, Kawamura T, Terashima M, Kawata N, Tanaka M, Kakushima N, Takizawa K, Ono H.
-
Journal Title
Virchows Arch.
Volume: 印刷中
Issue: 2
Pages: 155-161
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-