Establishment of human cancer microenvironment mouse model
| Project/Area Number |
16K08742
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Experimental pathology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
OKADA SEIJI 熊本大学, エイズ学研究センター, 教授 (50282455)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 患者由来腫瘍移植モデル / 高度免疫不全マウス / 胆管細胞癌 / 悪性リンパ腫 / 固形癌 / がん微小環境 / 患者腫瘍移植マウスモデル / 原発性滲出性リンパ腫 / がん関連線維芽細胞 / 消化管間質腫瘍 / マウスモデル / 患者腫瘍組織移植モデル / 癌 / 動物 |
|---|
| Outline of Final Research Achievements |
We established cholangiocarcinoma lung and liver metastasis model, and showed that CD147 played important role for epithelial-mesenchymal transition and metastasis. We also established patient-derived xenograft (PDX) model of liver fluke derived cholangiocarcinoma. Five cell lines were established from 12 PDX samples.
We showed that autophagy is accelerated in Primary effusion lymphoma (PEL) and inhibition of autophagy induced apoptosis for PEL cells. We also showed that transcriptional factor, PU.1, is methylated in PEL cells and activation of PU.1 induced apoptosis for PEL cells. These results indicate that epigenetic regulation can be important target for treatment of PEL.
|
| Academic Significance and Societal Importance of the Research Achievements |
高度免疫不全マウスへの移植系を用いて難治性腫瘍であるヒト胆管細胞癌と悪性リンパ腫にヒト由来腫瘍細胞を移植する事でマウス生体内に「ヒトのがん微小環境」を構築した。本系は、ヒト腫瘍のAvatarとして難治性腫瘍の病態解析と治療薬開発に有用な系として期待される。また、悪性リンパ腫のエピジェネチックな制御機構の一端を解明し、新規治療法開発の可能性を示唆した。
|
Report
(4 results)
Research Products
(39 results)
-
-
-
-
-
-
-
[Journal Article] Cyclin D1 depletion interferes with oxidative balance and promotes cancer cell senescence.2018
Author(s)
Laphanuwat P, Likasitwatanakul P, Sittithumcharee G, Thaphaengphan A, Chomanee N, Suppramote O, Ketaroonrut N, Charngkaew K, Lam EW, Okada S, Panich U, Sampattavanich S, Jirawatnotai S.
-
Journal Title
Journal of Cell Science
Volume: 131
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] HIV positivity may not have a negative impact on survival in Epstein-Barr virus-positive Hodgkin lymphoma: A Japanese nationwide retrospective survey.2018
Author(s)
Yotsumoto M, Ito Y, Hagiwara S, Terui Y, Nagai H, Ota Y, Ajisawa A, Uehira T, Tanuma J, Ohyashiki K, Okada S.
-
Journal Title
Oncology Letters
Volume: 16
Pages: 3923-3928
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
[Journal Article] YM155 induces apoptosis through proteasome-dependent degradation of MCL-1 in primary effusion lymphoma.2017
Author(s)
Kojima Y, Hayakawa F, Morishita T, Sugimoto K, Minamikawa Y, Iwase M, Yamamoto H, Hirano D, Imoto N, Shimada K, Okada S, Kiyoi H
-
Journal Title
Pharmacol Res.
Volume: in press
Pages: 242-251
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
[Journal Article] Correlation between Histone Acetylation and Expression of Notch1 in Human Lung Carcinoma and Its Possible Role in Combined Small Cell Lung Carcinoma.2017
Author(s)
Hassan, W., Takebayashi, S. I., Abdalla, M. O., Fujino, K., Kudoh, S., Motooka, Y., Sato, Y., Naito, Y., Higaki, K., Wakimoto, J., Okada, S., Nakao, M., Ishikawa, Y., and Ito, T.
-
Journal Title
Lab. Invest.
Volume: 印刷中
Issue: 8
Pages: 913-921
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
