Mechanism of exosomal histone in neuroinflammation
| Project/Area Number |
16K08754
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
Kawakami Kyojiro 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (90589227)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エクソソーム / ミクログリア / 神経炎症 / プロテオーム / ヒストン |
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| Outline of Final Research Achievements |
In the present study, we aimed to understand the characteristics of exosomes released upon neuroinflammation from microglia in brain. First, we performed proteomic analysis of exosomes released from cultured microglial cells after LPS stimulation and identified proteins whose expressions were altered by the treatment. Second, we confirmed that exosomes released from activated microglial cells activated naive microglial cells. Lastly, we modified and developed exosome isolation methods from mouse brain and also found that cell-specific exosomes can be isolated from mouse brain by the immunocapture method. These findings will provide a basis for understanding the role of exosomes released from microglia upon neuroinflammation.
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| Academic Significance and Societal Importance of the Research Achievements |
脳内の細胞から放出されるエクソソームの役割はいまだ不明な点が多いが、エクソソームは標的細胞へ到達し、その細胞での遺伝子発現を変化させること等が報告されている。アルツハイマー病やパーキンソン病などの神経変性疾患で脳内におこる変化はエクソソームにも影響を与えるものと予想される。今回得られた基盤的な情報と方法論は、神経炎症に伴いミクログリアから分泌されるエクソソームの役割の解明および神経変性疾患の病態の理解に役立つものと考えられる。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Gamma-glutamyltransferase activity in exosomes as a potential marker for prostate cancer2017
Author(s)
Kyojiro Kawakami, Yasunori Fujita, Yoko Matsuda, Tomio Arai, Kengo Horie, Koji Kameyama, Taku Kato, Koichi Masunaga, Yutaka Kasuya, Masashi Tanaka, Kosuke Mizutani, Takashi Deguchi, Masafumi Ito
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Journal Title
BMC Cancer
Volume: 316
Issue: 1
Pages: 316-316
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Gamma-glutamyltransferase activity in exosomes as a marker for prostate and renal cell cancers.2017
Author(s)
Ito M, Kawakami K, Horie K, Fujita Y, Matsuda Y, Arai T, Kameyama K, Kato T, Masunaga K, Kasuya Y, Tanaka M, Deguchi T, Mizutani K.
Organizer
International Society for Extracellular Vesicles 2017
Related Report
Int'l Joint Research
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[Presentation] エクソソーム上γ-グルタミルトランスフェラーゼ活性の前立腺がんと前立腺肥大の鑑別における有用性2016
Author(s)
川上恭司郎, 藤田泰典, 松田陽子, 新井冨生, 堀江憲吾, 亀山紘司, 加藤卓, 桝永浩一, 粕谷豊, 田中雅嗣, 水谷晃輔, 出口隆, 伊藤雅史
Organizer
第39回日本分子生物学会年会
Place of Presentation
パシフィコ横浜、横浜
Year and Date
2016-11-30
Related Report
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[Presentation] Exosomal gamma-glutamyltransferase activity as a marker for prostate cancer2016
Author(s)
Ito M, Kawakami K, Fujita Y, Matsuda Y, Arai T, Horie K, Kameyama K, Kato T, Masunaga K, Kasuya Y, Tanaka M, Deguchi T, Mizutani K.
Organizer
2016 ASEMV annual meeting
Place of Presentation
Asilomar Conference Center, Monterey, USA
Year and Date
2016-10-20
Related Report
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