| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In the present study, we aimed to understand the characteristics of exosomes released upon neuroinflammation from microglia in brain. First, we performed proteomic analysis of exosomes released from cultured microglial cells after LPS stimulation and identified proteins whose expressions were altered by the treatment. Second, we confirmed that exosomes released from activated microglial cells activated naive microglial cells. Lastly, we modified and developed exosome isolation methods from mouse brain and also found that cell-specific exosomes can be isolated from mouse brain by the immunocapture method. These findings will provide a basis for understanding the role of exosomes released from microglia upon neuroinflammation.
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