| Project/Area Number |
16K08765
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平井 誠 順天堂大学, 医学部, 准教授 (50326849)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | マラリア原虫 / 薬剤耐性 / 適応度 / 種内競争 / アルテミシニン |
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| Outline of Final Research Achievements |
Acquisition of drug resistance with gene mutations generally raises decrease of fitness in organisms, and therefore, the drug resistance may be disadvantageous under conditions without the drug. To assess fitness costs imposed by resistant to sulfadoxine (SX) alone, we comprehensively evaluated parasite fitness within-hosts and within-vectors using a transgenic rodent malaria parasite Plasmodium berghei. Competitive experiments in which SX-resistant and wild-type parasites were co-infected into mice showed a similar growth rate. In contrast, growth rate was significantly greater in SX-resistant clones than that in wild-type clones within vectors. These observations suggest that acquisition of SX resistance did not alter the within-rodent fitness in the absence of the drug, whereas it unexpectedly provoked a fitness advantage within mosquitos.
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| Academic Significance and Societal Importance of the Research Achievements |
一般的に、遺伝子変異によって薬剤耐性を獲得した病原体には、適応度の低下が見られる。しかしながら本研究によって、マラリア原虫では薬剤耐性の獲得が適応度の低下を引き起こさない事例があるという、これまでの常識を覆す知見が得られた。本研究成果は、今後も抗マラリア薬を持続的かつ効果的に利用するための方策を考える上で参照されるべきものであり、国際保健に貢献するものである。
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