Elucidation of the micro-mechanism that resident Candida albicans invades and disseminates from intestinal epithelium.
| Project/Area Number |
16K08790
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Teikyo University |
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Principal Investigator |
ISHIJIMA SANAE 帝京大学, 医真菌研究センター, 講師 (80147214)
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| Co-Investigator(Kenkyū-buntansha) |
安部 茂 帝京大学, 医真菌研究センター, 教授 (10125974)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | Candida albicans / 消化管カンジダ症マウスモデル / 悪化因子 / Leaky Gut / アドヘシン / N-アセチルグルコサミン / キチンオリゴ糖 / タンパク分解酵素 / Mycobiome |
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| Outline of Final Research Achievements |
Focusing on GlcNAc which induces mycelial growth at the early stage of Candida infection, the relationship between Candida cells and chitin polymer (Ishijima SA; 2017) or chitin oligosaccharide (Ishijima SA; 2018) were elucidated. In addition, we detected high expression genes associated with morphological changes (Ishijima SA; 2018), and comprehensive analysis of infectious aggravating factors of Candida albicans in a murine gastrointestinal candidiasis model were performed (unpublished). Whole genome analysis of the clinical isolate TIMM 1768 strain used in the process of analysis was performed (Panthee S; 2019). Comparison with the standard strain SC5314 suggests the possibility of detecting a new deterioration factor. In addition, we have identified and developed a drug with a detoxifying effect on gastrointestinal candidiasis.
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| Academic Significance and Societal Importance of the Research Achievements |
マウスモデルを用いて、カンジダ菌が消化管粘膜から全身播種する経過を解析し、各臓器に感染拡大して発現する遺伝子を網羅的に解析（現在継続中）したことにより、新たな抗真菌薬開発の標的分子が選択され、真菌症の予防・治療効果に重要な役割を果たす可能性が得られた。また、高い抗真菌効果を持つ乳酸菌を添加することで、腸内細菌叢を正常化し、真菌感染症を制御することを検証し、有望な結果が得られたことから、真菌症の新たな予防・治療法開発の方向性が示唆された。真菌症は、今後さらに増加する傾向にあるので、本研究の成果は感染予防・治療法の開発の基礎として役立つと考えられる。
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Report
(4 results)
Research Products
(11 results)
