| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
It is difficult to fix the field of view of the lungs due to the effects of respiratory movements and heart beats. In order to overcome these anatomical problems, this study developed and created a new accessory for fixing the lung to the objective lens. The reporter influenza virus (Color-flu) developed in this study infected the bronchial epithelium and alveolar epithelium in mice, and fluorescent proteins were produced in the infected cells. We succeeded in detecting infected cells in the lungs in live mice using Color-flu. Furthermore, we analyzed the interaction and kinetics of immune cells and influenza-infected cells in the lungs by using a reporter mouse that produced fluorescent proteins specifically in neutrophils and macrophages.
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