| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
The influenza viruses have segmented single-stranded negative-sense RNAs (vRNAs) as their genomes. Each vRNA segment is encapsidated by viral nucleoproteins and an RNA-dependent RNA polymerase to form a ribonucleoprotein complex (vRNP) with a rod-shaped, double-helical structure. The vRNA is either transcribed into mRNA or replicated into complementary RNA (cRNA) in context of the vRNP. However, the vRNP conformation during transcription and replication remains unknown. Here, we employed high-speed atomic force microscopy to visualize native structure of vRNPs producing viral RNA in vitro. Our analysis showed two types of vRNPs that are associated with newly synthesized RNAs; 1) intact rod-shaped helical vRNP connecting with a structured RNA and 2) deformed vRNP associated with a looped double-stranded RNA. Our findings provide important insights into viral RNA synthesis by influenza virus vRNPs and suggest mechanisms for transcription and replication of the influenza virus genome.
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