| Project/Area Number |
16K08968
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
亀崎 豊実 自治医科大学, 医学部, 教授 (90316513)
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| Research Collaborator |
HATTORI Yukio
NAKAMORI Yosiki
OHTO Fukumi
TAKAGI Fumiya
MORI Kentaro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 赤血球結合IgG / クームス試験 / フローサイトメートリー / AIHA / MFID / フローサイトメトリー / IgG1 / IgG3 / 自己免疫性溶血性貧血 / Coombs試験陰性 |
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| Outline of Final Research Achievements |
Autoimmune hemolytic anemia (AIHA) is a hemolytic disease where autoantibody against self is produced, and it gives rise to in vivo hemolysis. Coombs test is employed for the diagnosis of the AIHA. However, Coombs test can't quantitatively measure the antibody (IgG). A method that measures quantitatively red cell-bound antibody by RIA is useful in that respect. However, it is difficult for the RIA method to be applied to routine analysis. Therefore, we developed a flow cytometric assay.Average red blood cell bound IgG numbers were expressed in units of MFID. MFID of normal individuals (n=80), demonstrates normal distribution, and reference value was set to less than 9.72. Coombs negative and positive AIHA cases were beyond it. Some AIHA samples that became Coombs negative after steroid treatment also displayed positive in the MFID. It is available for estimation of effectiveness of the therapy for AIHA.
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| Academic Significance and Societal Importance of the Research Achievements |
今回開発したクームス試験に代わるフローサイトメートリーによる赤血球結合IgG測定法は日常検査として導入が可能である。また、感度もクームス試験より高く、定量性もあるためAIHAなどの診断、治療効果や予後判定に利用可能である。新規の検査法として広く利用されることを期待する。
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