Elucidation of mechanisms of peripheral itch hypersensitivity focusing on dorsal root ganglia and development for its therapeutic application
Project/Area Number |
16K08997
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pain science
|
Research Institution | Juntendo University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
冨永 光俊 順天堂大学, 医学(系)研究科(研究院), 先任准教授 (50468592)
鎌田 弥生 順天堂大学, 医学(系)研究科(研究院), 助教 (00410035)
梅原 芳恵 順天堂大学, 医学(系)研究科(研究院), 博士研究員 (40707072)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | かゆみ過敏 / サテライトグリア / アトピー性皮膚炎 / 感覚神経 / リポカリン2 / サテライトグリア細胞 / かゆみ / 後根神経節 / 難治性かゆみ |
Outline of Final Research Achievements |
This study was performed to reveal the roles of satellite glial cells (SGC) derived lipocalin-2 (LCN2) in the pathogenesis of atopic dermatitis (AD) using an AD model NC/Nga mouse. AD-like symptoms were induced by application of Dermatophagoides farinae body (Dfb) twice a week for 3 weeks. LCN2 mRNA and protein expressions in dorsal root ganglion (DRG) were higher in AD-NC/Nga- than control NC/Nga-mice. Immunohistochemically, LCN2 was co-localized with GLAST, a marker of SGC, in DRG. The number of LCN2-immunoreactive SGC was increased in the DRG of AD-NC/Nga mice. Expression level of LCN2 mRNA was increased faster in the DRG than the spinal cord during induction of dermatitis in NC/Nga mice. Intrathecally administrated anti-LCN2 antibody twice a week for 3 weeks reduced dermatitis score in AD-NC/Nga mice, but did not affect scratching behavior. Thus, SGC derived lipocalin-2 may be involved in the pathogenesis of dermatitis in AD-NC/Nga mice.
|
Academic Significance and Societal Importance of the Research Achievements |
近年、世界的にアトピー性皮膚炎(AD)の有病率は上昇しており、本研究領域への国民の関心も非常に高い。このような背景において、後根神経節(DRG)とsatellite glial cell(SGC)の相互作用に着眼した本研究成果は体性感覚分野の発展に役立つとともに、将来、『難治性かゆみの克服』と『QOL向上』に向けたADの新規治療法の開発に結び付くことが期待される。
|
Report
(3 results)
Research Products
(64 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] A possible role for CD26/DPPIV enzyme activity in the regulation of psoriatic pruritus2017
Author(s)
Komiya, E. Hatano, R. Otsuka, H. Itoh, T. Yamazaki, H. Yamada, T. Dang, N. H. Tominaga, M. Suga, Y. Kimura, U. Takamori, K. Morimoto, C. Ohnuma, K.
-
Journal Title
J Dermatol Sci
Volume: 印刷中
Issue: 3
Pages: 212-221
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Accumulation of immunoglobulin G against Dermatophagoides farinae tropomyosin in dorsal root ganglia of NC/Nga mice with atopic dermatitis-like symptoms.2017
Author(s)
Otsu A, Kawasaki H, Tominaga M, Shigenaga A, Matsuda H, Takahashi N, Nakajima T, Naito H, Baba T, Ogawa H, Tomooka Y, Yamakura F, Takamori K.
-
Journal Title
Biochem Biophys Res Commun.
Volume: 485
Issue: 4
Pages: 707-712
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] The effectiveness of new triple combination therapy using synthetic disease-modifying anti-rheumatic drugs with different pharmacological function against rheumatoid arthritis: the verification by an in vitro and clinical study.2017
Author(s)
Hirai T, Ikeda K, Fujishiro M, Tsushima H, Hayakawa K, Suzuki S, Yamaguchi A, Nozawa K, Morimoto S, Takasaki Y, Ogawa H, Takamori K, Tamura N, Sekigawa I
-
Journal Title
Clin Rheumatol
Volume: 36
Issue: 1
Pages: 51-58
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
[Journal Article] Definition of Sensitive Skin: an Expert Position Paper from the Special Interest Group on Sensitive Skin of the International Forum for the Study of Itch.2016
Author(s)
Misery L, Stander S, Szepietowski JC, Reich A, Wallengren J, Evers AW, Takamori K, Brenaut E, Gall-Ianotto CL, Fluhr J, Berardesca E, Weisshaar E.
-
Journal Title
Acta Derm Venereol
Volume: 未定
Issue: 1
Pages: 4-6
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopic dermatitis.2016
Author(s)
Kamata Y, Kimura U, Matsuda H, Tengara S, Kamo A, Umehara Y, Iizumi K, Kawasaki H, Suga Y, Ogawa H, Tominaga M, Takamori K.
-
Journal Title
J Dermatol Sci
Volume: 84
Issue: 3
Pages: 266-271
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils2016
Author(s)
Nakayama H, Kurihara H, Morita YS, Kinoshita T, Mauri L, Prinetti A, Sonnino S, Yokoyama N, Ogawa H, Takamori K, Iwabuchi K
-
Journal Title
Sci Signal
Volume: 11
Issue: 449
Pages: 0-0
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Spinal itch transmission.2017
Author(s)
Takamori K.
Organizer
American Academy of Dermatology (AAD) 48th Annual Meeting
Place of Presentation
Convention center, Orland, FL, USA
Related Report
Int'l Joint Research / Invited
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-