• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Role of BEGAIN for central sensitization in the spinal dorsal horn

Research Project

Project/Area Number 16K09001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Pain science
Research InstitutionKansai Medical University

Principal Investigator

KATANO Tayo  関西医科大学, 医学部, 准教授 (60469244)

Co-Investigator(Kenkyū-buntansha) 伊藤 誠二  大阪医科大学, その他部局等, 客員教授 (80201325)
西田 和彦  関西医科大学, 医学部, 助教 (80448026)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords慢性疼痛 / 脊髄後角 / BEGAIN / NMDA受容体 / 神経障害性疼痛 / in situ hybridization / mRNA / 後シナプス / 記憶・学習 / 海馬 / EPSC / 神経
Outline of Final Research Achievements

To clarify the molecular function of brain enriched guanylate kinase associated protein (BEGAIN) in the spinal dorsal horn, we performed electrophysiological recording of NMDA receptor, immunohistochemistry for BEGAIN and synaptic marker proteins, PSD-95 and synaptophysin, and in situ hybridization for BEGAIN in the spinal cord. In these analyses, we identified the relationship between BEGAIN and NMDA receptor as a delay of time to peak of EPSC in SG neurons of BEGAIN knockout mouse and the synaptic localization of BEGAIN in the spinal laminae Iii-IIIo. These results and previous results by us indicate that BEGAIN is involved in the mechanical allodynia in neuropathic pain concerned with the action of NMDA receptor. Furthermore, broad distribution of BEGAIN-positive neurons in not only spinal dorsal horn but also spinal ventral horn was demonstrated by in situ hybridization.

Academic Significance and Societal Importance of the Research Achievements

これまで、機能が不明であったBEGAINという蛋白質を、慢性疼痛の発症維持に関与する分子として、詳細な分子機序について解析をおこなった。そしてBEGAINは、脊髄のニューロンにおいてシナプスに局在し、NMDA受容体との機能的相互作用を介し、疼痛病態での異常感覚の発生に関与することを明らかにした。慢性疼痛に関わるタンパク質の同定と機能解析は、将来の創薬につながる能性があることから、BEGAINの研究成果と今後のより詳細なBEGAINの分子基盤における成果は、疼痛発症機序の解明という学術的意義だけでなく、慢性疼痛患者を対照とした創薬研究の創出という社会的意義も大きいものである。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Proteomic identification of novel neuropathic pain-related proteins in the postsynaptic density (PSD) fraction of the spinal dorsal horn2018

    • Author(s)
      片野 泰代
    • Journal Title

      生化学

      Volume: 90 Issue: 6 Pages: 810-814

    • DOI

      10.14952/SEIKAGAKU.2018.900810

    • NAID

      40021783164

    • ISSN
      0037-1017
    • Year and Date
      2018-12-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of Caskin1 protein and phenotypic characterization of its knockout mice using a comprehensive behavioral test battery2018

    • Author(s)
      Katano Tayo、Takao Keizo、Abe Manabu、Yamazaki Maya、Watanabe Masahiko、Miyakawa Tsuyoshi、Sakimura Kenji、Ito Seiji
    • Journal Title

      Molecular Brain

      Volume: 11 Issue: 1 Pages: 63-63

    • DOI

      10.1186/s13041-018-0407-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two isoforms of cyclic GMP-dependent kinase-I exhibit distinct expression patterns in the adult mouse dorsal root ganglion.2018

    • Author(s)
      Uchida Hitoshi、Matsumura Shinji、Katano Tayo、Watanabe Masahiko、Schlossmann Jens、Ito Seiji
    • Journal Title

      Molecular Pain

      Volume: 14 Pages: 1-11

    • DOI

      10.1177/1744806918796409

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Impaired peripheral nerve regeneration in type-2 diabetic mouse model.2018

    • Author(s)
      Pham M. V., Nguyen H. T., Katano, T., Matsumura, S., Aaito, S., Yamada A., Furue, H. and Ito, S.
    • Journal Title

      European Journal of Neuroscience

      Volume: 47 Issue: 2 Pages: 126-139

    • DOI

      10.1111/ejn.13771

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of Brain-Enriched Guanylate Kinase-Associated Protein (BEGAIN) in Chronic Pain after Peripheral Nerve Injury.2016

    • Author(s)
      Katano T, Fukuda M, Furue H, Yamazaki M, Abe M, Watanabe M, Nishida K, Yao I, Yamada A, Hata Y, Okumura N, Nakazawa T, Yamamoto T, Sakimura K, Takao T, Ito S.
    • Journal Title

      eNeuro.

      Volume: 3 Issue: 5 Pages: 0110-16

    • DOI

      10.1523/eneuro.0110-16.2016

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Protein Distribution and functional characterization of CASK-interacting protein 1 (Caskin1) in mice2018

    • Author(s)
      Tayo Katano, Keizo Takao, Manabu Abe, Maya Yamazaki, Masahiko Watanabe, Tsuyoshi Miyakawa, Kenji Sakimura, and Seiji Ito
    • Organizer
      The 48th annual meeting of the Society for Neuroscience
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CASK-interacting protein 1 (Caskin1)の中枢神経系における機能的特徴の解析2018

    • Author(s)
      Tayo Katano, Keizo Takao, Manabu Abe, Maya Yamazaki, Masahiko Watanabe, Tsuyoshi Miyakawa, Kenji Sakimura and Seiji Ito
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Search of BEGAIN-binding proteins in the brain2017

    • Author(s)
      Katano, T. Ito S.
    • Organizer
      The 47th annual meeting of the Society for Neuroscience
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脊髄後角における神経障害性疼痛関連分子BEGAINの同定2017

    • Author(s)
      片野泰代、福田正史、山崎真弥、阿部学、渡辺雅彦、矢尾育子、奥村宣明、中澤敬信、山本雅、崎村建司、高尾敏文、伊藤誠二
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Biological characterization of mouse BEGAIN in hippocampus2016

    • Author(s)
      片野泰代,渡辺雅彦,崎村建司,伊藤誠二
    • Organizer
      第89回日本生化学学会
    • Place of Presentation
      仙台国際センター、宮城
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi