An epidemiological study about a role for the intestinal microbiota in the pathogenesis of atherosclerosis in a population of community-dwelling Japanese.

• Project/Area Number: 16K09088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Kurume University
• Principal Investigator: Fukami Ako 久留米大学, 医学部, 講師 (90449926)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 住民検診 / 腸内細菌 / 動脈硬化 / 疫学 / 腸内フローラ / 生活習慣病 / 疫学研究

Outline of Final Research Achievements

This study was demonstrated to investigate a role for the intestinal microbiota in the pathogenesis of atherosclerosis. We couldn’t perform comprehensive analysis about
intestines bacterial flora, but we investigated that associations between metabolites of enteric bacteria or inflammatory biomarkers and atherosclerotic parameters in a general population. Plasma levels of trimethylamine-N-oxide levels, one of intestines bacterial flora, were tendency associated with metabolic factors. Moreover faecal Chitinase 3-like 1 and calprotectin levels, inflammatory biomarkers in the intestine, were significantly associated with urinary albumin-to-creatinine ratio, it suggested that intestinal inflammation may be involved in endothelial dysfunction.

Academic Significance and Societal Importance of the Research Achievements

本研究では、腸内細菌代謝産物と血清動脈硬化関連因子との統計学的な有意な関連を明らかにすることはできなかったが、メタボリック症候群の構成因子との正の傾向にあることは示された。また、腸内環境に関わる腸管内の炎症性バイオマーカーと尿中微量アルブミンとの関連は、腸管内の炎症と血管内の炎症および血管内皮機能との関連を示唆する結果と考えられる。動脈硬化性疾患において、腸内細菌により作られた腸内環境が影響する可能性を示唆する結果であると考え、今後の更なる研究に寄与する意義ある結果であったと考える。

Research Products

• [Journal Article] Thrombospondin-2 as a Potential Risk Factor in a General Population (2019)
  • Author(s): Morikawa Nagisa、Adachi Hisashi、Enomoto Mika、Fukami Ako、Kumagai Eita、Nakamura Sachiko、Nohara Yume、Nakao Erika、Kono Shoko、Tsuru Tomoko、Sakaue Akiko、Hamamura Hitoshi、Fukumoto Yoshihiro
  • Journal Title: International Heart Journal Volume: 60 Issue: 2 Pages: 310-317
  • DOI: 10.1536/ihj.18-246
  • NAID: 130007616768
  • ISSN: 1349-2365, 1349-3299
  • Year and Date: 2019-03-30
  • Peer Reviewed / Open Access
• [Journal Article] Twenty-four-hour urinary potassium excretion, but not sodium excretion, is associated with all-cause mortality in a general population. (2018)
  • Author(s): Nohara-Shitama Y, Adachi H, Enomoto M, Fukami A, Kumagai E, Nakamura S, Kono S, Morikawa N, Nakao E, Sakaue A, Tsuru T, Fukumoto Y
  • Journal Title: J Am Heart Assoc Volume: 4 Issue: 1
  • DOI: 10.1161/jaha.117.007369
  • Peer Reviewed / Open Access
• [Journal Article] Association between physical activity, occupational sitting time and mortality in a general population: An 18-year prospective survey in Tanushimaru, Japan (2018)
  • Author(s): Sakaue Akiko、Adachi Hisashi、Enomoto Mika、Fukami Ako、Kumagai Eita、Nakamura Sachiko、Nohara Yume、Kono Shoko、Nakao Erika、Morikawa Nagisa、Tsuru Tomoko、Hamamura Hitoshi、Yoshida Noriko、Fukumoto Yoshihiro
  • Journal Title: European Journal of Preventive Cardiology Volume: in press Issue: 7 Pages: 758-766
  • DOI: 10.1177/2047487318810020
  • Peer Reviewed / Open Access
• [Journal Article] Elevated plasma transforming growth factor β1 levels predict the development of hypertension in normotensives: The 14-year follow-up study. (2017)
  • Author(s): Nakao E, Adachi H, Enomoto M, Fukami A, Kumagai E, Nakamura S, Nohara Y, Kono S, Sakaue A, Morikawa N, Tsuru T, Fukumoto Y
  • Journal Title: Am J Hypertens. Volume: 30（8） Issue: 8 Pages: 808-814
  • DOI: 10.1093/ajh/hpx053
  • Peer Reviewed
• [Journal Article] Impact of cystatin C and microalbuminuria on cognitive impairment in the population of community-dwelling Japanese. (2017)
  • Author(s): Kono S, Adachi H, Enomoto M, Fukami A, Kumagai E, Nakamura S, Nohara Y, Morikawa N, Nakao E, Sakaue A, Tsuru T, Fukumoto Y
  • Journal Title: Atherosclerosis. Volume: 265 Pages: 71-77
  • Peer Reviewed
• [Journal Article] A useful tool as a medical checkup in a general population -Bioelectrical impedance analysis- (2017)
  • Author(s): Enomoto M, Adachi H, Fukami A, Kumagai E, Nakamura S, Nohara Y, Kono S, Nakao E, Morikawa N, Tsuru T, Sakaue A, Fukumoto Y
  • Journal Title: Front Cardiovasc Med Volume: 4 Pages: 3-3
  • DOI: 10.3389/fcvm.2017.00003
  • Peer Reviewed / Open Access
• [Journal Article] Serum Albumin and Cerebro-cardiovascular Mortality During a 15-year Study in a Community-based Cohort in Tanushimaru, a Cohort of the Seven Countries Study (2016)
  • Author(s): Umeki Y, Adachi H, Enomoto M, Fukami A, Nakamura S, Nohara Y, Nakao E, Sakaue A, Tsuru T, Morikawa N, Fukumoto Y
  • Journal Title: Internal Medicine Volume: 55 Issue: 20 Pages: 2917-2925
  • DOI: 10.2169/internalmedicine.55.6931
  • NAID: 130005430000
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Augmentation index (AI) in a dose-response relationship with smoking habits in males – The Tanushimaru Study ; (2016)
  • Author(s): Tsuru T, Adachi H, Enomoto M, Fukami A, Kumagai E, Nakamura S, Nohara Y, Kono S, Nakao E, Sakaue A, Morikawa N, Fukumoto Y
  • Journal Title: Medicine (Baltimore) Volume: 95 Issue: 51 Pages: e5368-e5368
  • DOI: 10.1097/md.0000000000005368
  • Peer Reviewed / Open Access
• [Presentation] An association between plasma and urinary levels of trimethylamine-N-oxide (TMAO) and metabolic factors in a population of community-dwelling Japanese. (2017)
  • Author(s): AKO FUKAMI
  • Organizer: 85th EAS Congress
  • Place of Presentation: Prague Congress Centre
  • Year and Date: 2017-04-23
  • Int'l Joint Research