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Molecular epidemiological study of pneumococcal serotypes, drug resistance, and surface antigen for novel vaccine development

Research Project

Project/Area Number 16K09101
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Hygiene and public health
Research InstitutionSapporo Medical University

Principal Investigator

Kawaguchiya Mitsuyo  札幌医科大学, 医学部, 助教 (70733062)

Co-Investigator(Kenkyū-buntansha) 小林 宣道  札幌医科大学, 医学部, 教授 (80186759)
漆原 範子  札幌医科大学, 医学部, 准教授 (80396308)
Research Collaborator ITO Masahiko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords肺炎球菌 / 蛋白結合型ワクチン / 表層蛋白 / 血清型 / 非ワクチン血清型 / 分子疫学 / 薬剤耐性 / ワクチン
Outline of Final Research Achievements

After the introduction of the pneumococcal conjugate vaccine (PCV) for children, an increase in pneumococcal diseases caused by non-PCV serotypes is a worldwide concern. The prevalence of serotypes, antimicrobial resistance, and its relevance to PspA which is a considered as promising vaccine candidate were investigated for 729 (678 children, 51 adults) non-invasive pneumococcal isolates. Isolates were collected in hospitals and clinics in Hokkaido between June and November 2016. As a results, 87.9% of isolates from children belonged to non-PCV13 serotypes and most isolates (81-100%) of the dominant serotypes 15A, 35B and 23A were non-susceptible to penicillin. While most of the isolates (99%) had PspA belonging to either family 1 or 2, PspA family 3/clade 6 was detected in only non-PCV13 serotype 37 isolates assigned to ST447 and ST7970 showing the mucoid phenotype.

Academic Significance and Societal Importance of the Research Achievements

小児に対する肺炎球菌結合型ワクチン導入後、国内外においてワクチンに含まれない血清型の増加とその薬剤耐性傾向が臨床上問題となっている。この現況に対し、肺炎球菌血清型分布とその薬剤耐性の状況、流行株の分子疫学的特徴を明らかにすることで、今後の肺炎球菌感染症対策に寄与する知見を得る。加えて、莢膜型(血清型)に依存しない、次世代肺炎球菌ワクチン抗原の候補である菌表層蛋白PspAの分子疫学的性状を含めた研究を行なう。これらの成果を国内外で発信・報告することは、新規ワクチン開発の早期の実現に結び付く基礎資料として、クローバルな肺炎球菌感染症対策に貢献するものであると考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (21 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Association between pneumococcal surface protein A (PspA) family and genetic/antimicrobial resistance traits of non-invasive pneumococcal isolates from adults in northern Japan2019

    • Author(s)
      Kawaguchiya M, Urushibara N, Aung MS, Habadera S, Ito M, Kudo K, Kobayashi N.
    • Journal Title

      Microbial Drug Resiatce

      Volume: 印刷中 Issue: 5 Pages: 744-751

    • DOI

      10.1089/mdr.2018.0267

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of pneumococcal surface protein A (PspA) in pediatric isolates of non-conjugate vaccine serotypes in Japan2018

    • Author(s)
      Kawaguchiya M, Urushibara N, Aung MS, Morimoto S, Ito M, Kudo K, Kobayashi N.
    • Journal Title

      Journal of Medical Microbiology

      Volume: 67 Issue: 8 Pages: 1130-1138

    • DOI

      10.1099/jmm.0.000775

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serotype distribution, antimicrobial resistance, and prevalence of pilus islets in pneumococci following the use of conjugate vaccines.2017

    • Author(s)
      Kawaguchiya M, Urushibara N, Aung MS, Shinagawa M, Takahashi S, Kobayashi N.
    • Journal Title

      Journal of Medical Microbiology

      Volume: 66 Issue: 5 Pages: 643-650

    • DOI

      10.1099/jmm.0.000479

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 平成29年度北海道公衆衛生協会賞表彰論文「北海道における非侵襲性感染症由来肺炎球菌の血清型と薬剤耐性に関する分子疫学的研究」2017

    • Author(s)
      川口谷充代
    • Journal Title

      北海道公衆衛生学雑誌

      Volume: 31 Pages: 22-24

    • Related Report
      2017 Research-status Report
  • [Journal Article] グローバル感染症と薬剤耐性菌対策の動向~最近のMRSA、薬剤耐性肺炎球菌の特徴について~2017

    • Author(s)
      小林宣道、川口谷充代、漆原範子、メイジ ソウアウン
    • Journal Title

      産業医学ジャーナル

      Volume: 40 Pages: 52-57

    • Related Report
      2017 Research-status Report
  • [Journal Article] Multidrug resistance in non-PCV13 serotypes of Streptococcus pneumoniae in northern Japan2017

    • Author(s)
      Kawaguchiya M, Urushibara N, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance

      Volume: 23 Issue: 2 Pages: 206-214

    • DOI

      10.1089/mdr.2016.0054

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] First report of serotype 23B Streptococcus pneumoniae isolated from an adult patient with invasive infection in Japan2017

    • Author(s)
      Takamatsu A, Kawaguchiya M, Chang B, Ito M, Hirano Y, Katsuta S, Matsuzaka S, SerizawaY, Kobayashi N.
    • Journal Title

      New Microbes New Infections

      Volume: 17 Pages: 96-97

    • DOI

      10.1016/j.nmni.2017.02.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Genetic diversity of pneumococcal surface protein A (PspA) in non-invasive pneumococcal isolates2019

    • Author(s)
      Kawaguchiya M, Urushibara N, Aung MS, Kobayashi N.
    • Organizer
      第92回日本細菌学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolated from adult patients in Hokkaido, northern main island of Japan2018

    • Author(s)
      Kawaguchiya M, Urushibara N, Aung MS, Kobayashi N.
    • Organizer
      17th Asia-Pacific Congress of Clinical Microbiology and Infection cum 8th International Infection Control Conference 2018 (APCCMI-IICC 2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ワクチン非含有血清型の侵襲性肺炎球菌感染症の2例2018

    • Author(s)
      土田晃輔、前田昂大、實川友美、川嶋雄平、朝倉啓文、酒井好幸、佐藤多嘉之、川口谷充代、小林宣道、布施茂登
    • Organizer
      第70回北日本小児科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小児非侵襲性感染症由来肺炎球菌の莢膜血清型と表層タンパク質 A(PspA)遺伝子の分布2018

    • Author(s)
      川口谷充代、小林宣道
    • Organizer
      第67回日本感染症学会東日本地方会学術集会 第65回日本化学療法学会東日本支部総会 合同学会2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] Epidemiological studies on the distribution of serotype and pneumococcal surface protein isolated from pediatric outpatients2018

    • Author(s)
      川口谷充代、小林宣道
    • Organizer
      第88回日本衛生学会学術総会
    • Related Report
      2017 Research-status Report
  • [Presentation] Invasive and non-invasive pneumococcal serotypes and antimicrobial resistance following the introduction of conjugate vaccines2017

    • Author(s)
      川口谷充代、漆原範子、小林宣道
    • Organizer
      第87回日本衛生学会学術総会
    • Place of Presentation
      シーガイアコンベンションセンター(宮崎)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 侵襲性および非侵襲性肺炎球菌の莢膜血清型と薬剤感受性および菌体表層の細菌性線毛pilusの分布2017

    • Author(s)
      川口谷充代、漆原範子、小林宣道
    • Organizer
      第91回日本感染症学会総会・学術講演会 第65回日本化学療法学会学術集会 合同学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Serotype distribution, antimicrobial resistance, and prevalence of pilus islets of Streptococcus pneumoniae in Hokkaido, Japan2017

    • Author(s)
      Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Nobumichi Kobayashi
    • Organizer
      International Union of Microbiological Societies Congresses 2017 (IUMS2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 2016年に分離された小児非侵襲性肺炎球菌の莢膜血清型と薬剤耐性遺伝子の分布状況2017

    • Author(s)
      川口谷充代、小林宣道
    • Organizer
      第76回日本公衆衛生学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 北海道で分離された小児非侵襲性肺炎球菌の血清型分布の経時的変化2017

    • Author(s)
      川口谷充代、漆原範子、森本茂雄、伊藤政彦、工藤兼司、鷲見紋子、小林宣道
    • Organizer
      第69回北海道公衆衛生学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Replacement with antibiotic-resistant serotypes of non-invasive Streptococcus pneumoniae in Hokkaido, Japan2017

    • Author(s)
      Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Shigeo Morimoto, Masahiko Ito, Kenji Kudo, Nobumichi Kobayashi
    • Organizer
      The 30th International Congress of Chemotherapy and Infection 2017 (ICC 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolated from a university hospital in Japan2016

    • Author(s)
      Kawaguchiya M, Urushibara N, Shinagawa M, Kobayashi N.
    • Organizer
      The 48th Asia-Pacific Academic Consortium for Public Health Conference
    • Place of Presentation
      Teikyo University, Tokyo, Japan
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Antimicrobial susceptibility and molecular analysis in nonpneumococcal conjugate vaccine serotypes of noninvasive S. pneumoniae isolated from children2016

    • Author(s)
      川口谷充代、漆原範子、小林宣道
    • Organizer
      第86回日本衛生学会学術総会
    • Place of Presentation
      旭川市民文化会館(旭川)
    • Year and Date
      2016-05-11
    • Related Report
      2016 Research-status Report
  • [Presentation] 13価肺炎球菌ワクチン(PCV13)非含有の血清型株における薬剤感受性とペニシリン耐性株の分子疫学的解析2016

    • Author(s)
      川口谷充代、漆原範子、小林宣道
    • Organizer
      第90回日本感染症学会・学術講演会
    • Place of Presentation
      仙台国際センター(仙台)
    • Year and Date
      2016-04-15
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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