Functional analysis of miR-301a in aging, metabolic syndrome and carcinogenesis

Research Project

Project/Area Number	16K09242
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	General internal medicine(including psychosomatic medicine)
Research Institution	Kagawa University
Principal Investigator	Fujita Koji 香川大学, 医学部附属病院, 病院助教 (50749421)
Co-Investigator(Kenkyū-buntansha)	正木勉香川大学, 医学部, 教授 (30335848) 森下朝洋香川大学, 医学部附属病院, 助教 (60423430)
Project Period (FY)	2016-04-01 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	microRNA / Transgenic mouse / miRNA / トランスジェニックマウス / 加齢 / 癌 / メタボリック症候群 / 老化 / 遺伝子
Outline of Final Research Achievements	We constructed a transgenic mouse by introducing a precursor of microRNA-301a in to a wild type mouse. MicroRNA expression analysis in three pair of 48-week old mice revealed that transgene positive mice strongly expressed miR-301a with statistical significance in their small intestines than transgene negative mice. The transgenic mouse presented potential to be a model in functional analysis of miR-301a in aging. However, further investigation using the transgenic mouse was halted because of shortness of research funding. In addition to the current study, we worked for in vitro and in vivo studies to prove whether suppression of tumor growths was correlated to miR-301a expression. As a result, we published three papers on journals.
Academic Significance and Societal Importance of the Research Achievements	本研究においては，加齢・メタボリック症候群・発がんに関連するmicroRNA-301aの遺伝子を導入したマウスを作出した．このマウスが48週齢に達したとき，遺伝子を導入していないマウスに比べて，小腸におけるmicroRNA-301aの発現が高いことが確認された．このマウスが加齢におけるmicroRNA-301aの機能を解析する上でのモデル動物となる可能性が示されたと考えられる．しかし予算の制約があり，これ以上の解析は困難と判断し，遺伝子組み換えマウスを用いた研究を終了した．本研究計画と並行して，市販されているがん細胞株及びマウスでの研究を進め，成果を三題の論文として発表済みである．