Molecular diagnostic markers for early gastric cancer using Human and H.Pylori genome alterations
Project/Area Number |
16K09295
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | St. Marianna University School of Medicine |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
山本 博幸 聖マリアンナ医科大学, 医学部, 准教授 (40332910)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 胃がん / 胃洗浄廃液 / ピロリ菌 / 遺伝子異常 / メチル化 / 次世代シークエンス解析 / パイロシークエンス / 挿入 / パックバイオ解析 / 23SrRNA / ゲノム解析 |
Outline of Final Research Achievements |
We developed a next-generation sequencing (NGS)-based method for genome wide SNPs analysis of Helicobacter pylori strains (HPs) specific genomes using gastric wash sample (G-Scan method). Gastric cancer-specific candidate 71 HP gene SNPs was obtained from analysis using the remaining 4 cases of gastric cancer, 4 cases of gastritis.
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Academic Significance and Societal Importance of the Research Achievements |
胃洗浄廃液内に混在した複数種ピロリ菌の全ゲノム解析(G-Scan法)により、「胃がんに特異的なピロリ菌遺伝子異常(OMPs: outer membrane proteins)」を版権することができた。これによるピロリ菌構造変化が胃粘膜定着強固と持続炎症に関与し、癌化を促し得る」可能性が推測され、今後は「ヒトゲノム+ピロリゲノム異常を分子マーカーとし、「廃液」による超早期胃がん診断を実現す」ため、前向き臨床試験の実施および、ピロリ菌遺伝子異常(OMPs)による発がんメカニズム解析を行う予定である。これにより、廃液を用いるだけで胃がん診断が可能となる。
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Identification of human papillomavirus (HPV) 16 DNA integration and the ensuing patterns of methylation in HPV-associated head and neck squamous cell carcinoma cell lines.2017
Author(s)
Takashi Hatano, Daisuke Sano, Hideaki Takahashi, Hiroshi Hyakusoku, Yasuhiro Isono, Shoko Shimada, Kae Sawakuma, Kentaro Takada, Ritsuko Oikawa, Yoshiyuki Watanabe, Hiroyuki Yamamoto, Fumio Itoh, Jeffrey N. Myers, Nobuhiko Oridate
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Journal Title
Internal Journal of Cancer
Volume: 140(7)
Issue: 7
Pages: 1571-1580
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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