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Analysis of the basal lamina fenestrations in intestinal villous epithelium and the relation to the regulation of nutrient absorption

Research Project

Project/Area Number 16K09303
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Gastroenterology
Research InstitutionNiigata University

Principal Investigator

YOKOYAMA Junji  新潟大学, 医歯学総合病院, 准教授 (70422615)

Co-Investigator(Kenkyū-buntansha) 寺井 崇二  新潟大学, 医歯学系, 教授 (00332809)
牛木 辰男  新潟大学, 医歯学系, 教授 (40184999)
本田 穣  新潟大学, 医歯学総合研究科, 客員研究員 (20643547)
川合 弘一  新潟大学, 医歯学総合研究科, 客員研究員 (80419291)
佐藤 祐一  新潟大学, 医歯学総合研究科, 客員研究員 (00401761)
Research Collaborator YAGI Kazuhiro  
AZUMI Rie  
MORITA Keisuke  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords小腸絨毛 / 脂肪吸収 / 基底膜 / 肥満 / 小腸絨毛基底膜 / 消化吸収 / 走査型電子顕微鏡 / 小腸機能
Outline of Final Research Achievements

We investigated the ultrastructure of the intestinal villi in the rat by scanning electron microscopy. Our study showed that free cells adhering to the fenestrations increased in the upper jejunum two hours after feeding high-fat diet and the size of the fenestrations in this region also increased after feeding high-fat diet for 2 days. This enlargement of fenestrations may play an important role in increasing the efficiency of lipid absorption by facilitating the movement of chylomicrons from the intercellular space to the lamina propria. Similar changes were also observed in the study of human small intestine villi. In addition, we observed jejunal white spots of lymphangiectasia by magnified balloon enteroscopy. We are planning to further examine the relationship between white villi, which is seen by obese people, and the adjustment function of fenestrations in relation to the white spots of lymphangiectasia.

Academic Significance and Societal Importance of the Research Achievements

電子顕微鏡を用いた小腸絨毛基底膜の観察を行った論文は数少ない。窓の存在は知られていたが、その役割や食事による評価に関する報告はなく、ヒトにおける窓の評価に関しても皆無であった。小腸からの脂肪吸収メカニズムについては、乳糜管への回収、吸収の面からも研究がすすめられており、Zhangらはカイロミクロンの吸収は中心乳糜管の内皮細胞間接着の強さにより変化し、この接着の強さをVEGF-Aが制御していることを報告している。しかし、基底膜の窓に注目した研究報告はなく、今回の成果は、脂肪吸収メカニズムの解明に大きく貢献できるものと考える。また、本研究は、肥満の病態解明という重要テーマに通じる研究である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (4 results)

  • [Journal Article] Dynamic changes in basal lamina fenestrations in rat intestinal villous epithelium under high-fat diet condition2019

    • Author(s)
      MORITA Keisuke、AZUMI Rie、SATO Masatoshi、MIZUTANI Yusuke、HAYATSU Manabu、TERAI Shuji、USHIKI Tatsuo
    • Journal Title

      Biomedical Research

      Volume: 40 Issue: 2 Pages: 57-66

    • DOI

      10.2220/biomedres.40.57

    • NAID

      130007632091

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2019-04-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Magnified Single-Balloon Enteroscopy Enables Observation of Jejunal White Spots Associated with Lymphangiectasia2018

    • Author(s)
      Tominaga Kentaro、Tsuchiya Atsunori、Kawata Yuzo、Yokoyama Junji、Terai Shuji
    • Journal Title

      Digestive Diseases

      Volume: 37 Issue: 2 Pages: 170-174

    • DOI

      10.1159/000493578

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Usefulness of Capsule Endoscopy and Double-balloon Enteroscopy for the Diagnosis of Multiple Carcinoid Tumors in the Small Intestine: Case Reports and a Literature Review2018

    • Author(s)
      Tominaga K、Kamimura K、Yokoyama J、Terai S
    • Journal Title

      Internal Medicine

      Volume: 58 Pages: 655-659

    • NAID

      130007606549

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <b>Dynamics of basal lamina fenestrations in the rat intestinal villous epithelium in response to dietary </b><b>conditions </b>2018

    • Author(s)
      Azumi R, Morita K, Mizutani Y, Hayatsu M, Terai S, Ushiki T.
    • Journal Title

      Biomedical Research

      Volume: 39 Issue: 2 Pages: 65-74

    • DOI

      10.2220/biomedres.39.65

    • NAID

      130006708565

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 消化管運動機能検査としての小腸カプセル内視鏡の有用性の検討2018

    • Author(s)
      冨永 顕太郎、横山 純二、寺井 崇二
    • Organizer
      日本消化器病学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 当院における小腸カルチノイド7例の臨床的検討2018

    • Author(s)
      冨永 顕太郎、川田 雄三、本田 穣、上村 顕也、横山 純二、寺井 崇二ほか
    • Organizer
      大腸癌研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 重篤な基礎疾患を持たないサイトメガロウイルス小腸炎の特徴と小腸内視鏡の有用性2018

    • Author(s)
      横山 純二、冨永 顕太郎、寺井 崇二
    • Organizer
      日本消化器病学会大会(JDDW 2018)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ラット小腸絨毛の上皮下基底膜にみられる窓:その食事による経時的変化の検討2018

    • Author(s)
      佐藤公俊、森田景介、安住里映、榎本初音、立木秀尚、寺井崇二、牛木辰男
    • Organizer
      第123回日本解剖学会総会全国学術集会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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