Mechanism of liver fibrosis induced by HBV infection
| Project/Area Number |
16K09366
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Iijima Sayuki 名古屋市立大学, 大学院医学研究科, 研究員 (00416273)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 線維化 / HBV / 肝線維化 / 糖鎖 / 細胞ストレス / ウイルス / 感染症 / 慢性疾患 / B型肝炎 |
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| Outline of Final Research Achievements |
As a factor of liver fibrosis caused by HBV infection, I hypothesized that intracellular stress increased by viral infection. When the fluctuation of intracellular stress in the presence of HBV was examined in an in vitro experimental system, the endoplasmic reticulum stress which is a typical cell stress or the antioxidant action showed little fluctuation regardless of the increase or decrease of the viral load . Since it was difficult to verify the relationship between HBV and cell stress in vitro, we tried to use an experimental system that could cause liver damage in an in vivo system as the next step. In order to search for markers that can distinguish non-invasively from control group and liver injury group, sugar chain analysis was performed using each liver tissue and serum sample. Several marker candidates were confirmed.
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| Academic Significance and Societal Importance of the Research Achievements |
HBV感染による肝線維化は細胞ストレスと密接に関与していると推測されているが、その詳細は長年の研究にもかかわらず未だに明らかになっていない。本研究は、従来までのタンパク強制発現系の結果をさらに発展させ、生理的に近い状態でのHBV感染により細胞ストレスかどうかを検証する非常に重要、かつ独創的な研究である。本研究から、肝線維化に重要な役割を果たす細胞、ウイルス因子、シグナル伝達経路が明らかになると予想される。肝線維化は肝硬変・肝癌の前段階と考えられているため、その進展の機序解明による社会的貢献は非常に多大である。
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Report
(4 results)
Research Products
(5 results)
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[Journal Article] Hepatic IFNL4 expression is associated with non-response to interferonbased therapy through the regulation of basal interferon-stimulated gene expression in chronic hepatitis C patients.2017
Author(s)
Murakawa M, Asahina Y, Kawai-Kitahata F, Nakagawa M, Nitta S, Otani S, Nagata H, Kaneko S, Asano Y, Tsunoda T, Miyoshi M, Itsui Y, Azuma S, Kakinuma S, Tanaka Y, Iijima S, Tsuchiya K, Izumi N, Tohda S, Watanabe M.
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Journal Title
J Med Virol
Volume: in press
Issue: 7
Pages: 1241-1247
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] 3.Hepatocellular carcinoma-associated core N51H mutation with BCP PC mutations in HBV genotype F1b enhances infectivity and up-regulates C-Myc and GAB22016
Author(s)
Hayashi S, Khan AA, Ogawa K, Kawashima K, Simons-Petrusa B, Homan CE, McMahon BJ, Murakami S, Iijima S, Isogawa M, Watanabe T, Tanaka Y
Organizer
The 67th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2016)
Place of Presentation
The United States of America, Boston
Year and Date
2016-11-11
Related Report
Int'l Joint Research
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[Presentation] 遺伝子型の異なるB型肝炎ウイルス株に対するワクチン免疫後中和抗体の感染防御能の検討2016
Author(s)
堤進, 飯尾悦子, 渡邊綱正, 村上周子, 五十川正記, 飯島沙幸, 井上貴子, 松波加代子, 田尻和人, 小澤龍彦, 田中靖人
Organizer
第26回抗ウイルス療法学会総会
Place of Presentation
名古屋東急ホテル, 愛知, 名古屋
Year and Date
2016-05-13
Related Report
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