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Evaluation of the intestinal microbiota of patients with heart failure using metagenomic sequencing

Research Project

Project/Area Number 16K09433
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Cardiovascular medicine
Research InstitutionOsaka University

Principal Investigator

Tsukamoto Yasumasa  大阪大学, 医学部附属病院, 特任助教(常勤) (80747072)

Co-Investigator(Kenkyū-buntansha) 大谷 朋仁  大阪大学, 医学系研究科, 講師 (30623897)
Research Collaborator Katsimichas Themistoklis  
Nakamoto Kei  
Nakamura Kenji  
Taniguchi Tatsunori  
Iida Tetsuya  
Nakamura Shota  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords心不全 / 腸内細菌叢 / メタゲノム / 腸内細菌
Outline of Final Research Achievements

Non-ischemic heart failure (HF) patients and healthy controls were assessed by 16S rRNA analysis of bacterial DNA extracted from stool samples. After processing of sequencing data, bacteria were taxonomically classified, diversity indices were used to examine microbial ecology, and relative abundances of genera were compared between groups. Furthermore, we predicted gene carriage for bacterial metabolic pathways. The most abundant genus in both groups was Bacteroides. β diversity metrics revealed a separation of the groups across components of variation. Streptococcus and Veillonella were enriched in the common core microbiota of patients, while SMB53 was depleted. Metabolic pathways variation showed differences between groups.
In conclusion, non-ischemic HF patients exhibited multidimensional differences in intestinal microbial communities compared with healthy subjects.

Academic Significance and Societal Importance of the Research Achievements

今回の研究により、非虚血性心不全患者では健常者と比して腸内細菌叢に差異を生じていたことが明らかになった。今後これらの結果から、腸内細菌叢や栄養状態の変化と心不全の病態との関連についての知見がさらに深まっていくとともに、将来的にプロバイオティクスや抗菌薬治療による腸内細菌叢への介入が心不全治療の新たな標的として発展していくことが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 4 results) Book (1 results)

  • [Journal Article] Non-Ischemic Heart Failure With Reduced Ejection Fraction Is Associated With Altered Intestinal Microbiota2018

    • Author(s)
      Katsimichas T, Ohtani T, Motooka D, Tsukamoto Y, Kioka H, Nakamoto K, Konishi S, Chimura M, Sengoku K, Miyawaki H, Sakaguchi T, Okumura R, Theofilis K, Iida T, Takeda K, Nakamura S, Sakata Y.
    • Journal Title

      Circulation Journal

      Volume: 82 Issue: 6 Pages: 1640-1650

    • DOI

      10.1253/circj.CJ-17-1285

    • NAID

      130006744246

    • ISSN
      1346-9843, 1347-4820
    • Year and Date
      2018-05-25
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-Ischemic Heart Failure With Reduced Ejection Fraction Is Associated With Altered Intestinal Microbiota.2018

    • Author(s)
      Katsimichas T, Ohtani T, Tsukamoto Y, Sakata Y, et al.
    • Journal Title

      Circulation Journal

      Volume: -

    • NAID

      130006744246

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 心不全における心腸肝連関2018

    • Author(s)
      大谷 朋仁
    • Organizer
      第66回 日本心臓病学会学術集会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Baseline and 1-year Follow-up Serial Optical Coherence Tomography Evaluation of Coronary Plaques in Heart Transplant Recipients2018

    • Author(s)
      Yasumasa Tsukamoto
    • Organizer
      ISHLT 38th ANNUAL MEETING & SCIENTIFIC SESSIONS
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Heart Failure is Associated with an Altered Intestinal Microbiome2018

    • Author(s)
      Themistoklis Katsimichas, Yasumasa Tsukamoto, Yasushi Sakata, et al.
    • Organizer
      The 82th Annual Meeting of the Japanese Ciculation Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Usefulness of Liver Stiffness Measurements for Evaluation of Central Venous Pressure in Adult Congenital Heart Disease2018

    • Author(s)
      Yasumasa Tsukamoto
    • Organizer
      The 82th Annual Meeting of the Japanese Ciculation Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Serial evaluation of plaque characteristics in coronary artery lesions using optical coherence tomography after heart transplantation2017

    • Author(s)
      Yasumasa Tsukamoto
    • Organizer
      International Society of Heart and Lung Transplantation 37th Annual Meeting & Scientific Sessions
    • Place of Presentation
      Manchester Grand Hyatt Hotel, San Diego, 米国
    • Year and Date
      2017-04-06
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Plaque Morphology in Coronary Artery after Heart Transplantation Assessed by Serial Optical Coherence Tomography (シンポジウム)2017

    • Author(s)
      Yasumasa Tsukamoto
    • Organizer
      第81回日本循環器学会学術集会
    • Place of Presentation
      石川県立音楽堂
    • Year and Date
      2017-03-19
    • Related Report
      2016 Research-status Report
  • [Presentation] 当院ACHD 症例における肝硬度と中心静脈圧の検討2017

    • Author(s)
      塚本 泰正
    • Organizer
      第19回日本成人先天性心疾患学会総会・学術集会
    • Place of Presentation
      ホテルグリーンパーク津
    • Year and Date
      2017-01-14
    • Related Report
      2016 Research-status Report
  • [Presentation] 高度の蛋白尿によりエベロリムス中止を余儀なくされた心肺移植後の症例2016

    • Author(s)
      塚本 泰正
    • Organizer
      第35回日本心臓移植研究会学術集会
    • Place of Presentation
      ホテルさっぽろ芸文館
    • Year and Date
      2016-10-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 当院における心臓移植後悪性疾患の経験2016

    • Author(s)
      塚本 泰正
    • Organizer
      第52回日本移植学会総会
    • Place of Presentation
      グランドプリンスホテル高輪
    • Year and Date
      2016-10-01
    • Related Report
      2016 Research-status Report
  • [Book] 腸内細菌やその生体機能に介入する循環器疾患治療2019

    • Author(s)
      平田健一, 山下智也, 大谷朋仁, 阿部高明
    • Total Pages
      7
    • Publisher
      CARDIAC PRACTICE 2019 29 4 303-309
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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