Intracardiac/extracardiac circulating microRNA profiling in atrial fibrillation -cardiac specific biomarkers for atrial remodeling-

• Project/Area Number: 16K09462
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Fujita Health University
• Principal Investigator: Harada Masahide 藤田医科大学, 医学部, 准教授 (70514800)
• Co-Investigator(Kenkyū-buntansha): 奥崎 大介 大阪大学, 微生物病研究所, 助教 (00346131) ; 尾崎 行男 藤田医科大学, 医学部, 教授 (50298569) ; 渡邉 英一 藤田医科大学, 医学部, 教授 (80343656)
• Research Collaborator: Koshikawa Masayuki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マイクロRNA / 心房細動 / 心房リモデリング / バイオマーカー / 循環器・高血圧 / 不整脈

Outline of Final Research Achievements

The blood samples were collected from the coronary sinus (CS, reflecting cardiac phenomena more specifically) and the central venous (CV) in patients with and without atrial fibrillation (AF); the plasma expression of microRNAs (miRNAs) was measured using quantitative PCR and next generation sequencer (NGS). We identified miRNAs that showed significant differences in the plasma expression between patients with and without AF in the CS samples. Among them, we focused on miRNAs that also showed similar expression differences in the CV samples; these were candidates for the clinical biomarkers. We created the novel miRNA-based method to assess AF pathophysiology and prospectively evaluate the efficacy in terms of whether it can predict therapeutic outcomes, disease progression, and adverse event risk.

Academic Significance and Societal Importance of the Research Achievements

血中miRNAによる新たなAFの評価法が確立されれば、病態に基づいた個別化医療が実現できる可能性がある。そして、AFの治療効果、病態の進行、合併症の発症などが正確に予測できるようになれば、治療成績や生命予後を劇的に改善させる可能性がある。また、臨床指標を用いた病態評価法は、基礎研究でのmiRNAの新知見を臨床的に確認するツールとなり、学術的な意義も極めて大きい。AFは実臨床で最も頻度の高い不整脈でり、本研究によってAFの臨床マネージメントが革新的に進歩すれば、社会的、医療経済的な貢献度は計り知れない。

Research Products

• [Journal Article] Impact of serum albumin levels on supratherapeutic PT-INR control and bleeding risk in atrial fibrillation patients on warfarin: A prospective cohort study. (2019)
  • Author(s): Kawai M, Harada M, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Journal Title: Int J Cardiol Heart Vasc. Volume: 22 Pages: 111-116
  • DOI: 10.1016/j.ijcha.2019.01.002
  • Peer Reviewed / Open Access
• [Journal Article] Left Atrial Appendage Thrombus Prior to Atrial Fibrillation Ablation in the Era of Direct Oral Anticoagulants (2018)
  • Author(s): Harada Masahide、Koshikawa Masayuki、Motoike Yuji、Ichikawa Tomohide、Sugimoto Kunihiko、Watanabe Eiichi、Ozaki Yukio
  • Journal Title: Circulation Journal Volume: 82 Issue: 11 Pages: 2715-2721
  • DOI: 10.1253/circj.CJ-18-0398
  • NAID: 40021697183
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2018-10-25
  • Peer Reviewed / Open Access
• [Journal Article] Serum microRNA-126 and -223 as new-generation biomarkers for sarcoidosis in patients with heart failure. (2018)
  • Author(s): Fujiwara W, Kato Y, Hayashi M, Sugishita Y, Okumura S, Yoshinaga M, Ishiguro T, Yamada R, Ueda S, Harada M, Naruse H, Ishii J, Ozaki Y, Izawa H.
  • Journal Title: J Cardiol. Volume: 72 Pages: 452-457
  • Peer Reviewed
• [Journal Article] Predicting acute kidney injury using urinary liver-type fatty-acid binding protein and serum N-terminal pro-B-type natriuretic peptide levels in patients treated at medical cardiac intensive care units. (2018)
  • Author(s): Naruse H, Ishii J, Takahashi H, et al.
  • Journal Title: Critical Care Volume: 22 Issue: 1 Pages: 197-197
  • DOI: 10.1186/s13054-018-2120-z
  • Peer Reviewed / Open Access
• [Journal Article] Thromboembolisms in atrial fibrillation and heart failure patients with a preserved ejection fraction (HFpEF) compared to those with a reduced ejection fraction (HFrEF) (2018)
  • Author(s): Sobue Yoshihiro、Watanabe Eiichi、Lip Gregory Y. H.、Koshikawa Masayuki、Ichikawa Tomohide、Kawai Mayumi、Harada Masahide、Inamasu Joji、Ozaki Yukio
  • Journal Title: Heart and Vessels Volume: 33 Issue: 4 Pages: 403-412
  • DOI: 10.1007/s00380-017-1073-5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice (2018)
  • Author(s): Takemoto Y, Horiba M, Harada M, Sakamoto K, Takeshita K, Murohara T, Kadomatsu K, Kamiya K.
  • Journal Title: Circulation Journal Volume: 82 Issue: 1 Pages: 19-27
  • DOI: 10.1253/circj.CJ-17-0043
  • NAID: 130006281926
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic Considerations in Atrial Fibrillation ― Mechanistic Insights and Therapeutic Opportunities ― (2017)
  • Author(s): Harada M, Melka J, Sobue Y, Nattel S.
  • Journal Title: Circulation Journal Volume: 81 Issue: 12 Pages: 1749-1757
  • DOI: 10.1253/circj.CJ-17-1058
  • NAID: 130006219238
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic Value of Combination of Plasma D-Dimer Concentration and Estimated Glomerular Filtration Rate in Predicting Long-Term Mortality of Patients With Stable Coronary Artery Disease (2017)
  • Author(s): Naruse H, Ishii J, Takahashi H, Kitagawa F, Okuyama R, Kawai H, Muramatsu T, Harada M, Yamada A, Motoyama S, Matsui S, Hayashi M, Sarai M, Watanabe E, Izawa H, Ozaki Y.
  • Journal Title: Circulation Journal Volume: 81 Issue: 10 Pages: 1506-1513
  • DOI: 10.1253/circj.CJ-16-1272
  • NAID: 130006099870
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Physically triggered Takotsubo cardiomyopathy has a higher in-hospital mortality rate. (2017)
  • Author(s): Sobue Y, Watanabe E, Ichikawa T, Koshikawa M, Yamamoto M, Harada M, Ozaki Y.
  • Journal Title: International Journal of Cardiology Volume: 235 Pages: 87-93
  • DOI: 10.1016/j.ijcard.2017.02.090
  • Peer Reviewed
• [Journal Article] Combination of high-sensitivity troponin I and N-terminal pro-B-type natriuretic peptide predicts future hospital admission for heart failure in high-risk hypertensive patients with preserved left ventricular ejection fraction (2017)
  • Author(s): Ryunosuke Okuyama
  • Journal Title: Heart and Vessels Volume: 印刷中 Issue: 7 Pages: 880-892
  • DOI: 10.1007/s00380-017-0948-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Assessment of trough rivaroxaban concentrations on markers of coagulation activation in nonvalvular atrial fibrillation population. (2017)
  • Author(s): Kitagawa F, Ishii J, Hiramitsu S, Takahashi H, Okuyama R, Kawai H, Muramatsu T, Harada M, Motoyama S, Naruse H, Matsui S, Sarai M, Hayashi M, Watanabe E, Izawa H, Ozaki Y.
  • Journal Title: Heart and Vessels Volume: 32 Issue: 5 Pages: 609-617
  • DOI: 10.1007/s00380-016-0912-0
  • Peer Reviewed
• [Journal Article] Prognostic importance of novel oxygen desaturation metrics in patients with heart failure and central sleep apnea. (2017)
  • Author(s): Watanabe, E., Kiyono, K., Matsui, S., Somers, V. K., Sano, K.,Hayano, J., Ichikawa, T., Kawai, M., Harada, M., Ozaki, Y.
  • Journal Title: J Card Fail Volume: 23 Issue: 2 Pages: 131-137
  • DOI: 10.1016/j.cardfail.2016.09.004
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Intracellular angiotensin-II interacts with nuclear angiotensin receptors in cardiac fibroblasts and regulates RNA synthesis, cell proliferation, and collagen secretion. (2017)
  • Author(s): Tadevosyan A, Xiao J, Surinkaew S, Naud P, Merlen C, Harada M, Qi X, Chatenet D, Fournier A, Allen BG, Nattel S.
  • Journal Title: Journal of the American Heart Association Volume: 印刷中 Issue: 4
  • DOI: 10.1161/jaha.116.004965
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic Value of Combination of Plasma D-Dimer Concentration and Estimated Glomerular Filtration Rate in Predicting Long-Term Mortality of Patients with Stable Coronary Artery Disease. (2017)
  • Author(s): Naruse H, Ishii J, Takahashi H, Kitagawa F, Okuyama R, Kawai H, Muramatsu T, Harada M, Yamada A, Motoyama S, Matsui S, Hayashi M, Sarai M, Watanabe E, Izawa H, Ozaki Y.
  • Journal Title: Circulation Journal Volume: 印刷中
  • NAID: 130006099870
  • Peer Reviewed / Open Access
• [Presentation] Next Generation Sequencing Reveals Intra‐cardiac/Extra‐cardiac MicroRNA Transcriptomes in Atrial Fibrillation. (2018)
  • Author(s): Masahide Harada, Daisuke Okuzaki, Jun Takeda, Yuji Motoike, Masayuki Koshikawa, Eiichi Watanabe, Yukio Ozaki.
  • Organizer: American Heart Association Scientific Session 2018
  • Int'l Joint Research
• [Presentation] Pulmonary Vein-dependent Electrophysiological Heterogeneity Underlies Localized Reentry and Atrial Fibrillation Provoked by Single Extra Stimulation: Implications of Anatomical Characteristics in Electrophysiology-. (2018)
  • Author(s): Harada M, Motoike Y, Asami F, Takeda J, Ichikawa T, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2018
  • Int'l Joint Research
• [Presentation] MicroRNA in Atrial Fibrillation: from Basic Mechanism to Translational Potential.-. (2018)
  • Author(s): Harada M.
  • Organizer: The 11th Asian Pacific Heart Rhythm Society.
  • Int'l Joint Research / Invited
• [Presentation] Current Status of Molecular Study in AF: From Basic Mechanisms to Translational Potential. (2018)
  • Author(s): Harada M.
  • Organizer: The 11th Asian Pacific Heart Rhythm Society.
  • Int'l Joint Research / Invited
• [Presentation] Metabolic Consideration in Atrial Fibrillation: Mechanistic Insight And Therapeutic Opportunities. (2018)
  • Author(s): Harada M.
  • Organizer: The 11th Asian Pacific Heart Rhythm Society.
  • Int'l Joint Research / Invited
• [Presentation] Left Pulmonary Vein-dependent Electrophysiological Heterogeneity Underlies Localized Reentry and Atrial Fibrillation Provoked by Single Extra Stimulation: Implications of Anatomical Characteristics in Electrophysiology-. (2018)
  • Author(s): Harada M, Motoike Y, Asami F, Takeda J, Ichikawa T, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2018
  • Int'l Joint Research
• [Presentation] Shortened Refractoriness and Conduction Slowing Associated with Elevated Left-atrial Pressure Confers Atrial Fibrillation Sustainability in Patients with Sleep Apnea Syndrome. (2018)
  • Author(s): Motoike Y, Harada M, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2018
  • Int'l Joint Research
• [Presentation] Next Generation Sequencing Reveals Intra-cardiac/Extra-cardiac Circulating MicroRNA Transcriptomes in Atrial Fibrillation. (2018)
  • Author(s): Harada M, Okuzaki D, Takeda J, Motoike Y, Koshikawa M, Ichikawa T, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Feasibility of Switching to Dabigatran on the Day of Catheter Ablation in Atrial Fibrillation Patients Taking Direct Oral Anticoagulants. (2018)
  • Author(s): Harada M, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] High-Resolution Electro-anatomical Mapping-guided Procedure Decreases Residual Gap and Acute Reconnection in Pulmonary Vein Isolation for Atrial Fibrillation. (2018)
  • Author(s): Koshikawa M, Harada M, Motoike Y, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Obstructive and Central Sleep Apnea Have Different Impacts on Left-Atrial Arrhythmogenic Electrophysiology in Paroxysmal Atrial Fibrillation. (2018)
  • Author(s): Motoike Y, Harada M, Fujiwara A, Takeda J, Ichikawa T, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Peripheral Perfusion Reactivity to Sinus Rhythm Restoration after Catheter Ablation in Patients with Persistent Atrial Fibrillation. (2018)
  • Author(s): Motoike Y, Harada M, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Prognostic Implication of Selvester QRS Scoring in Atrial Fibrillation Patients with HFrEF Undergoing Catheter Ablation. (2018)
  • Author(s): Nomura Y, Harada M, Kitagawa F, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 82nd Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Thrombolytic Efficacy of Dabigatran in Patients Undergoing Atrial Fibrillation Ablation -Peri-procedural Left-atrial Thrombus in the Era of Direct Oral Anticoagulants-. (2017)
  • Author(s): Harada M, Koshikawa M, Motoike Y, Makino T, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 石川県立音楽堂 他(石川県 金沢市）
  • Year and Date: 2017-03-17
• [Presentation] Semi-automatic Selvester QRS Scoring Can Estimate Scar Lesion in Patients with Cardiac Sarcoidosis -Development of Novel ECG Analysis Software- (2017)
  • Author(s): Harada M, Kitagawa F, Sobue Y, Motoike Y, Makino T, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 石川県立音楽堂 他(石川県 金沢市）
  • Year and Date: 2017-03-17
• [Presentation] Shortened Refractoriness and Conduction Slowing Associated with Elevated Left-atrial Pressure Confers Atrial Fibrillation Sustainability in Patients with Sleep Apnea Syndrome. (2017)
  • Author(s): Motoike Y, Harada M, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 石川県立音楽堂 他(石川県 金沢市）
  • Year and Date: 2017-03-17
• [Presentation] Thrombolytic Efficacy of Dabigatran in Patients Undergoing Atrial Fibrillation Ablation -Peri-procedural Left-atrial Thrombus in the Era of Direct Oral Anticoagulants-. (2017)
  • Author(s): Harada M, Koshikawa M, Motoike Y, Makino T, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2017
  • Int'l Joint Research
• [Presentation] Shortened Refractoriness and Conduction Slowing Associated with Elevated Left-atrial Pressure Confers Atrial Fibrillation Sustainability in Patients with Sleep Apnea Syndrome. (2017)
  • Author(s): Motoike Y, Harada M, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2017
  • Int'l Joint Research
• [Presentation] Age- and Obesity-related Epicardial Adipose Tissue Accumulation Remodels Autonomic Balance and Increases Inflammation in Paroxysmal Atrial Fibrillation Patients. (2017)
  • Author(s): Ichikawa T, Harada M, Takeda J, Motoike Y, Makino T, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2017
  • Int'l Joint Research
• [Presentation] Inappropriate Dose Reduction of Direct Oral Anticoagulants Increases the Risk of Peri-procedural Left-atrial Thrombus in Patients Undergoing Atrial Fibrillation Ablation. (2017)
  • Author(s): Harada M, Koshikawa M, Makino T, Motoike Y, Ichikawa T, Fujiwara A, Watanabe E, Ozaki Y.
  • Organizer: The 64st Annual Meeting of the Japanese Heart Rhythm Society/The 10th Asia Pacific Heart Rhythm Society Scientific Session.
• [Presentation] Serum albumin levels predict major bleeding events associated with increased PT-INR in atrial fibrillation patients on warfarin. (2017)
  • Author(s): Yamamoto M, Harada M, Makino T, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 64st Annual Meeting of the Japanese Heart Rhythm Society/The 10th Asia Pacific Heart Rhythm Society Scientific Session.
• [Presentation] Age- and Obesity-related Epicardial Adipose Tissue Accumulation Remodels Autonomic Balance and Increases Inflammation in Paroxysmal Atrial Fibrillation Patients. (2017)
  • Author(s): Ichikawa T, Harada M, Takeda J, Makino T, Motoike Y, Koshikawa M, Watanabe E, Ozaki Y.
  • Organizer: The 64st Annual Meeting of the Japanese Heart Rhythm Society/The 10th Asia Pacific Heart Rhythm Society Scientific Session.
• [Presentation] Intracardiac and Extracardiac Expression of Pro-fibrotic MicroRNAs in Atrial Fibrillation -Implication for Cardiac Specific Biomarkers for Atrial Remodeling-. (2017)
  • Author(s): Harada M, Motoike Y, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society
• [Presentation] Thrombolytic Efficacy of Dabigatran in Patients Undergoing Atrial Fibrillation Ablation -Peri-procedural Left-atrial Thrombus in the Era of Direct Oral Anticoagulants-. (2017)
  • Author(s): Harada M, Koshikawa M, Motoike Y, Makino T, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society.
• [Presentation] Semi-automatic Selvester QRS Scoring Can Estimate Scar Lesion in Patients with Cardiac Sarcoidosis; Development of Novel ECG Analysis Software-. (2017)
  • Author(s): Harada M, Kitagawa F, Sobue Y, Motoike Y, Makino T, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 81st Annual Scientific Meeting of the Japanese Circulation Society.
• [Presentation] Human LA and PV Electrophysiology in AF -clinical implication for the pathophysiology- (2016)
  • Author(s): Harada M.
  • Organizer: Asia Pacific Heart Rhythm Society 2016
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-10-12
  • Int'l Joint Research / Invited
• [Presentation] MicroRNA as potential therapeutic target and biomarkers for atrial fibrillation. (2016)
  • Author(s): Harada M.
  • Organizer: Asia Pacific Heart Rhythm Society 2016
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2016-10-12
  • Int'l Joint Research / Invited
• [Presentation] Intracardiac and extracardiac expression of pro-fibrotic microRNAs in atrial fibrillation -implication for cardiac specific biomarkers for atrial remodeling- (2016)
  • Author(s): Harada M, Matsuo C, Motoike Y, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: European Society of Cardiology Congress 2016
  • Place of Presentation: Roma, Italy
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] Rate-dependent Shortening of Refractory Period with Conduction Slowing in the Left Pulmonary Vein Increases Atrial Vulnerability in Paroxysmal AF Patients. (2016)
  • Author(s): Harada M, Motoike Y, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Sawasaki K, Muto M, Murohara T, Watanabe E, Ozaki Y.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
• [Presentation] Electrophysiological Characteristics of the Left Atrium and Pulmonary Vein Confers Development of Atrial Fibrillation in Patients with Sleep Apnea Syndrome. (2016)
  • Author(s): Motoike Y, Harada M, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
• [Presentation] Passive Oscillation and Its Amplitude of Inter-atrial Septum in Transesophageal Echocardiography is a Novel Non-invasive Parameter for Left Atrial Pressure in Atrial Fibrillation. (2016)
  • Author(s): Makino T, Yamamoto M, Harada M, Motoike Y, Koshikawa M, Ichikawa T, Watanabe E, Ozaki Y.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
• [Presentation] MicroRNAs as Potential Therapeutic Target and Biomarkers for Atrial Fibrillation. (2016)
  • Author(s): Harada M, Watanabe E, Ozaki Y.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
  • Invited
• [Presentation] Autonomic Nerve-mediated Atrial Fibrillation: Clinical Characteristics and Mechanistic Implications. (2016)
  • Author(s): Harada M.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
  • Invited
• [Presentation] Urinary 3-methylhystidine is a Surrogate Maker for Inflammation-related Physical Frailty in Atrial Fibrillation. (2016)
  • Author(s): Koshikawa M, Harada M, Motoike Y, Makino T, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: The 31st Annual Meeting of the Japanese Heart Rhythm Society
  • Place of Presentation: 札幌コンベンションセンター(北海道 札幌市）
  • Year and Date: 2016-07-14
• [Presentation] Shortening of Effective Refractory Period with Conduction Slowing in Left Pulmonary Vein Increases Vulnerability to Paroxysmal Atrial Fibrillation. (2016)
  • Author(s): Harada M, Motoike Y, Fujiwara A, Makino T, Koshikawa M, Ichikawa T, Kawai M, Sawasaki K, Muto M, Murohara T, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2017
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2016-05-04
  • Int'l Joint Research
• [Presentation] Serum MicroRNA26a Levels Progressively Decrease during AF Development in Coronary Vein Blood Samples. (2016)
  • Author(s): Harada M, Motoike Y, Makino T, Koshikawa M, Ichikawa T, Kawai M, Watanabe E, Ozaki Y.
  • Organizer: Heart Rhythm 2017
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2016-05-04
  • Int'l Joint Research
• [Book] 心房細動と心房リモデリング－病態機序とマイクロRNAの関与－ Bio Clinica (2018)
  • Author(s): 原田将英
  • Total Pages: 7
  • Publisher: 北隆館
• [Book] 心房細動 （山下 武編） 心房細動における血栓塞栓症予防 (2018)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 最新医学社
• [Book] 臨床医のための循環器診療 心臓自律神経と心房細動-病態の理解とアブレーションへの応用- (2018)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 学樹書院
• [Book] 私の治療 2017－2018年度版 Wolf-Parkinson-White (WPW) 症候群 (2017)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 日本医事新報社
• [Book] Circulation Up-to Date Books 17 超実践的！ 抗凝固薬の使い方 （池田隆徳 編）、 ワルファリン (2017)
  • Author(s): 原田将英、渡邊英一
  • Publisher: メディカ出版
• [Book] 循環器ジャーナル 心電図スキルアップ (2017)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 医学書院
• [Book] Circulation Up-to Date Books 17 超実践的！ 抗凝固薬の使い方 (2017)
  • Author(s): 原田将英、渡邊英一
  • Publisher: メディカ出版
• [Book] 循環器ジャーナル 心電図診断スキルアップ (2017)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 医学書院
• [Book] 心不全ｘ不整脈 (2016)
  • Author(s): 原田将英、渡邊英一
  • Publisher: メディカ出版
• [Book] 薬局/特集 心房細動と抗血栓療法 (2016)
  • Author(s): 原田将英、渡邊英一
  • Publisher: 南山堂
• [Book] 循環器内科 心不全と心房細動 (2016)
  • Author(s): 市川智英、原田将英、渡邉英一
  • Publisher: 科学評論社