The role of gap junction-mediated regulation of endothelial cellular stiffness
| Project/Area Number |
16K09513
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Shimane University |
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Principal Investigator |
Okamoto Takayuki 島根大学, 学術研究院医学・看護学系, 准教授 (30378286)
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| Research Collaborator |
Suzuki Koji
Shimaoka Motomu
Kawamoto Eiji
Niibayashi Tomomi
Usuda Haruki
Tanaka Tetsuya
Wada Koichiro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 血管内皮細胞 / 細胞硬化 / ギャップ結合 / 細胞接着斑 / 細胞骨格 / 白血球接着 / 炎症 / 細胞の硬さ / 硬さ / 血管生物学 / 動脈硬化 / メカノバイオロジー |
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| Outline of Final Research Achievements |
The aim of this study is to investigate alteration in elastic modulus (stiffness) of vascular endothelial cells during inflammation using atomic force microscope, and to clarify the pathophysiological role of cellular stiffness. We found that vascular endothelial cells increased the stiffness during inflammatory stimulation. Here we have demonstrated that gap junction is involved in the regulation of cellular stiffness. Furthermore, we have shown that cellular stiffening induces leukocyte adhesion. Taken together, our results suggested the pathophysiological role of endothelial cellular stiffness.
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| Academic Significance and Societal Importance of the Research Achievements |
臨床的には、血管が硬くなると動脈硬化が進展し、心血管イベントが生じやすいことが知られている。本研究では、そのメカニズムの解明に取り組み、病態初期に見られる炎症時において血管内皮細胞そのものが硬化することを示し、その分子機構を解析した。さらに細胞が硬化した結果、白血球が硬化部位に集積する可能性を示し、血管硬化が血管病変の形成を促進する新しいメカニズムを明らかにした。
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Anti-adhesive effects of human soluble thrombomodulin and its domains2019
Author(s)
Kawamoto Eiji、Nago Nodoka、Okamoto Takayuki、Gaowa Arong、Masui-Ito Asami、Sakakura Yosuke、Akama Yuichi、Soe Zay Yar、Prajuabjinda Onmanee、Darkwah Samuel、Appiah Michael G.、Myint Phyoe Kyawe、Obeng Gideon、Park Eun Jeong、Imai Hiroshi、Shimaoka Motomu
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 511
Issue: 2
Pages: 312-317
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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