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Estimation of total nephron number in Japanese subjects and the comparison with other races in relation to kidney and cardiovascular diseases

Research Project

Project/Area Number 16K09631
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Kidney internal medicine
Research InstitutionJikei University School of Medicine

Principal Investigator

Tsuboi Nobuo  東京慈恵会医科大学, 医学部, 准教授 (40408422)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsネフロン数 / CKD
Outline of Final Research Achievements

A total of 64 nephron kidneys were used to estimate the total number of nephrons. It was clarified that the total number of nephrons in the Japanese has a large individual difference even in the normal renal function, and is closely related to the function of the kidney. Furthermore, it was found that individuals with a history of hypertension had a lower number of nephrons than normotensive. It was revealed that the total number of nephrons in the Japanese population (600,000 / kidney) was lower as compared to the results of similar studies conducted in other races. This study was the first to be conducted for Asians, and its results were highly evaluated at domestic and overseas academic societies in the field of kidney disease and hypertension.

Academic Significance and Societal Importance of the Research Achievements

総ネフロン数の差異は個人間の腎機能低下に対する感受性の違いのみならず、人種間でのCKDや末期腎不全の罹患率の違いにも影響することが予想される。既報の研究は、対象に多人種が混在しており、単一民族を対象とした検討は少ない。したがって、ほぼ単一民族とみなされる日本人を対象として総ネフロン数を計測する意義は大きい。
さらに、最近ではMRIを用いて腎の微細構造を解析し、総ネフロン数を非侵襲的に推定する試みもされている。このような新たな方法の確立においても、Stereologyを用いた総糸球体数の推算による検証は欠かせない。本研究結果はネフロン数研究における近未来的なアプローチを試みる上でも基礎となる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 10 results)

  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] Monash University(オーストラリア)

    • Related Report
      2016 Research-status Report
  • [Journal Article] Human Nephron Number, Hypertension, and Renal Pathology2019

    • Author(s)
      Go Kanzaki, Nobuo Tsuboi, Akira Shimizu, Takashi Yokoo
    • Journal Title

      Anat Rec (Hoboken)

      Volume: 15 Issue: 10 Pages: 24302-24302

    • DOI

      10.1002/ar.24302

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimation of nephron number in living humans by combining unenhanced computed tomography with biopsy-based stereology.2019

    • Author(s)
      Sasaki T, Tsuboi N, Okabayashi Y, Haruhara K, Kanzaki G, Koike K, Kobayashi A, Yamamoto I, Takahashi S, Ninomiya T, Shimizu A, Rule AD, Bertram JF, Yokoo T.
    • Journal Title

      Sci Rep.

      Volume: Oct 7;9(1) Issue: 1 Pages: 14400-14400

    • DOI

      10.1038/s41598-019-50529-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Group 1 innate lymphoid cells are involved in the progression of experimental anti-glomerular basement membrane glomerulonephritis and are regulated by peroxisome proliferator-activated receptor α.2019

    • Author(s)
      Okabayashi Y, Nagasaka S, Kanzaki G, Tsuboi N, Yokoo T, Shimizu A.
    • Journal Title

      Kidney Int.

      Volume: Oct;96(4) Issue: 4 Pages: 942-956

    • DOI

      10.1016/j.kint.2019.04.039

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aging Vs. Hypertension: An Autopsy Study of Sclerotic Renal Histopathological Lesions in Adults With Normal Renal Function.2019

    • Author(s)
      Okabayashi Y, Tsuboi N, Kanzaki G, Sasaki T, Haruhara K, Koike K, Takahashi H, Ikegami M, Shimizu A, Yokoo T.
    • Journal Title

      Am J Hypertens.

      Volume: Jun 11;32(7) Issue: 7 Pages: 676-683

    • DOI

      10.1093/ajh/hpz040

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synergistic Impact of Diabetes and Hypertension on the Progression and Distribution of Glomerular Histopathological Lesions.2019

    • Author(s)
      Sasaki T, Tsuboi N, Okabayashi Y, Haruhara K, Kanzaki G, Koike K, Takahashi H, Ikegami M, Shimizu A, Yokoo T.
    • Journal Title

      Am J Hypertens.

      Volume: Aug 14;32(9) Issue: 9 Pages: 900-908

    • DOI

      10.1093/ajh/hpz059

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Volume Ratio of Glomerular Tufts to Bowman Capsules and Renal Outcomes in Nephrosclerosis.2019

    • Author(s)
      Haruhara K, Tsuboi N*, Sasaki T, Amano H, Tanaka M, et al.
    • Journal Title

      Am J Hypertens

      Volume: 1;32(1) Issue: 1 Pages: 45-53

    • DOI

      10.1093/ajh/hpy147

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] New insights on glomerular hyperfiltration: a Japanese autopsy study2017

    • Author(s)
      Kanzaki G, Puelles VG, Cullen-McEwen LA, Hoy WE, Okabayashi Y, Tsuboi N, Shimizu A, Denton KM, Hughson MD, Yokoo T, Bertram JF
    • Journal Title

      JCI Insight

      Volume: 2(19) Issue: 19 Pages: 94334-94334

    • DOI

      10.1172/jci.insight.94334

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Single Nephron Parameters in Patients with IgA Nephropathy2019

    • Author(s)
      Marumoto, Hirokazu, Tsuboi, Nobuo, et al.
    • Organizer
      Annual meeting of American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impact of Nephron Number on Renal Uric Acid Excretion in Patients with IgA Nephropathy2019

    • Author(s)
      Marumoto, Hirokazu, Tsuboi, Nobuo, et al.
    • Organizer
      Annual meeting of American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Total Nephron Number and Renal Histopathological Lesions in IgA Nephropathy2019

    • Author(s)
      Marumoto, Hirokazu, Tsuboi, Nobuo, et al.
    • Organizer
      Annual meeting of American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of Tonsillectomy Monotherapy of Advanced-Stage IgA Nephropathy: A Case Series Study2019

    • Author(s)
      Marumoto, Hirokazu, Tsuboi, Nobuo, et al.
    • Organizer
      Annual meeting of American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Estimation of Nephron Number and Related Single Nephron Parameters in Patients with Idiopathic Membranous Nephropathy2019

    • Author(s)
      Okabayashi Yusuke, Tsuboi Nobuo et al
    • Organizer
      Annual meeting of American Society of Nephrology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Total nephron number and single nephron GFR in CKD subjects.2018

    • Author(s)
      Kanzaki G, Puelles V, Cullen-McEwen L, Okabayashi Y, Tsuboi N et al.
    • Organizer
      54th European Renal Association-European Dialysis Transplant Association Congress. Copenhagen.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Biopsy-based estimation of the total nephron number in Japanese healthy subjects.2018

    • Author(s)
      Sasaki T, Tsuboi N, Kanzaki G, Haruhara K, Okabayashi Y et al.
    • Organizer
      54th European Renal Association-European Dialysis Transplant Association Congress. Copenhagen.
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] TOTAL NEPHRON NUMBER DECREASES WITH THE STAGE OF CHRONIC KIDNEY DISEASE; A STUDY IN JAPANESE SUBJECTS2017

    • Author(s)
      G KANZAKI1,2, VG PUELLES3, LA CULLEN-MCEWEN1, Y OKABAYASHI2, N TSUBOI2, A SHIMIZU4, T YOKOO2, JF BERTRAM1
    • Organizer
      ANZSN ASM
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Total Nephron number decreases with the stage of chronic kidney disease; a study in Japanese subjects2017

    • Author(s)
      Go Kanzaki3, 4, Victor G. Puelles2, Luise A. Cullen-McEwen4, Yusuke Okabayashi3, Nobuo Tsuboi3, Akira Shimizu1, Takashi Yokoo3, John F. Bertram4
    • Organizer
      ASN
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Racial Differences in nephron number; Role of body size, kidney weight, and cortical volume in adult subjects among five populations2017

    • Author(s)
      Go Kanzaki1, 5, Victor G. Puelles3, Luise A. Cullen-McEwen1, Wendy E. Hoy4, Yusuke Okabayashi5, Nobuo Tsuboi5, Akira Shimizu2, Takashi Yokoo5, John F. Bertram1
    • Organizer
      ASN
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Low nephron number in Japanese subjects without overt renal disease: the effect of race and hypertension.2016

    • Author(s)
      Kanzaki G, Puelles VG, Gullen-McEwen LA, Hughson MD, Hoy WE, Okabayashi Y, Tsuboi N, Shimizu A, Yokoo T, Bertram JF.
    • Organizer
      Annual Meeting of American Society of Nephrology 2016
    • Place of Presentation
      Chicago USA
    • Year and Date
      2016-11-18
    • Related Report
      2016 Research-status Report
  • [Presentation] A clinical perspective of glomerular hyperfiltration in health and disease.2016

    • Author(s)
      Kanzaki G, Puelles VG, Gullen-McEwen LA, Hughson MD, Hoy WE, Okabayashi Y, Tsuboi N, Shimizu A, Yokoo T, Bertram JF.
    • Organizer
      Annual Meeting of American Society of Nephrology 2016
    • Place of Presentation
      Chicago USA
    • Year and Date
      2016-11-18
    • Related Report
      2016 Research-status Report
  • [Presentation] Low nephron number in Japanese subjects without overt renal disease.2016

    • Author(s)
      Kanzaki G, Puelles VG, Gullen-McEwen LA, Hughson MD, Hoy WE, Okabayashi Y, Tsuboi N, Shimizu A, Yokoo T, Bertram JF.
    • Organizer
      the 2016 Asian pacific congress of nephrology
    • Place of Presentation
      Perth, Australia
    • Year and Date
      2016-09-18
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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