| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Glomeruli were isolated by the sieving method from kidneys at 4 weeks of age of low birth weight (LBW) rat (N = 5) and normal birth weight ones (n = 5). The isolated glomeruli were cultured and primary culture of glomerular epithelial cells was also performed. Transcriptome analysis of RNA extracted from the obtained glomeruli and primary culture podocytes is outsourced. In the future, we plan to perform epigenetics analysis of the promoter region of the gene which should be expected as candidate genes by the bioinformatics analysis. In addition, by proteome analysis of LBW rat kidney, it was found that mTOR system should be upstream of mitochondrial dysfunction and glycolytic activation in LBW rats. The staining experiments by using antibodies against mTOR pathway revealed that mTOR pathway should be activated in human cases of low-birth related kidney disease.
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