Development of anti-idiotypic vaccine therapy for Alzheimer's disease
| Project/Area Number |
16K09696
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松原 悦朗 大分大学, 医学部, 教授 (70219468)
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| Research Collaborator |
Miura Yumako
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ワクチン / アルツハイマー型認知症 / アミロイドベータオリゴマー / 抗イディオタイプ抗体 / 神経病態免疫学 / 抗位ディオタイプ抗体 / 神経内科学 |
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| Outline of Final Research Achievements |
Aβ oligomers have been recognized as the true pathogenic molecules and the therapeutic target for Alzheimer's disease. This study aimed to develop a novel anti-idiotypic vaccine therapy for Alzheimer's disease using the anti-Aβ oligomer antibody that we have developed independently. Through the hybridoma technique, we obtained a few novel antibodies in vitro to neutralize the anti-Aβ oligomer antibody without neurotoxicity themselves, but we couldn't prove the activities as vaccines in vivo. Further study to reveal these antibodies structure will be need to design analogues with vaccine-like action.
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| Academic Significance and Societal Importance of the Research Achievements |
アルツハイマー型認知症の原因であるAβオリゴマーに似せた分子を免疫学的手法で作り出し，１回投与でアルツハイマー型認知症を予防できるワクチンの開発を目指した．独自に開発したAβオリゴマーの特異的抗体をマウスに免疫し，そのリンパ節の細胞を取り出して，抗体を作る能力を持つがん細胞（骨髄腫細胞）と融合させ，もとの抗体と結合する新しい抗体を選び出した（スクリーニング）．その結果，それ自身は毒性がなく，もとの抗体の機能を中和する，ごく少数の新規抗体が得られたが，安定して得ることは困難で，それが生体内で機能することは確認できなかった．今後はこの新規抗体の構造を検討し，生体内での有効性を確認する必要がある．
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Report
(4 results)
Research Products
(1 results)
