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Establishment of research fundation of IRF7 on the treatment of obesity and metabolic syndrome

Research Project

Project/Area Number 16K09753
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Metabolomics
Research InstitutionThe University of Tokushima

Principal Investigator

SAKAUE Hiroshi  徳島大学, 大学院医歯薬学研究部(医学域), 教授 (60372645)

Research Collaborator KURODA masashi  
TSUTSUMI rie  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords脂肪細胞 / 肥満 / メタボリックシンドローム / 転写因子 / IRF7 / ベージュ脂肪細胞 / UCP-1 / MCP-1 / 2型糖尿病 / 糖尿病
Outline of Final Research Achievements

Through the combination of DNA microarray and genomic data analysis to predict DNA binding motif, we have identified the transcription factor, Interferon Regulatory Factor 7 (IRF7) as a possible regulator of the genes related to adipocyte hypertrophy. To clarify the role of IRF7 in adipocytes, we found that enforced expression of IRF7 induced the transcription of Monocyte Chemoattractant Proteoin-1 (MCP-1), a key initial adipokine in chronic inflammation in obesity. CRISPR/Cas9 mediated-suppression of IRF7 leaded to significant reduction of MCP-1 mRNA. Luciferase assay and ChIP-PCR analysis shows that IRF7 transactivates MCP-1 gene. Taken together, our results suggest that IRF7 trans-activates MCP-1 mRNA in adipocyte, and might be involved in adipose tissue inflammation associated with obesity.

Academic Significance and Societal Importance of the Research Achievements

肥満形成とその病態発症機構における転写因子IRF7の役割が解明できうるとできたと考えられ、IRF7を分子標的とした新しい肥満・メタボリックシンドローム治療への意義を確立しえた。さらにTLR9-IRF7パスウエイを阻害する小分子化合物や薬剤の探索への基礎的基盤が確立した。TLR9-IRF7パスウエイを活性化するリガンド(DNAなど)を探索し、肥満モデルマウスに対して、受容体阻害薬の効果を検討することで、肥満とそれに伴う生活習慣病の治療に新たなアイデアが提供できたことからも、その社会的意義は大きい。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (33 results)

All 2019 2018 2017 2016 Other

All Journal Article (16 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 15 results,  Open Access: 10 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 4 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Role of orexin in exercise-induced leptin sensitivity in the mediobasal hypothalamus of mice.2019

    • Author(s)
      Shiuchi T, Miyatake Y, Otsuka A, Chikahisa S, Sakaue H
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 514 Pages: 166-172

    • NAID

      120006881396

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ADCY3遺伝子異常と肥満2019

    • Author(s)
      黒田 雅士 三島 優奈, 阪上 浩
    • Journal Title

      内分泌・糖尿病・代謝内科

      Volume: 48 Pages: 214-218

    • NAID

      40021856391

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effect of Janus kinase inhibition by tofacitinib on body composition and glucose metabolism.2018

    • Author(s)
      Chikugo M, Sebe M, Tsutsumi R, Iuchi M, KIshi J, Kuroda M, Harada N, Nishioka Y, Sakaue H
    • Journal Title

      J Med Invest.

      Volume: 65 Pages: 166-170

    • NAID

      130007495818

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Readthrough of ACTN3 577X nonsense mutation produces full-length α-actinin-3 protein.2018

    • Author(s)
      Harada N, Hatakeyama A, Okuyama M, Miyatake Y, Nakagawa T, Kuroda M, Masumoto S, Tsutsumi R, Nakaya Y, Sakaue H.
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 502 Pages: 422-428

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adipocyte Death and Chronic Inflammation in Obesity.2017

    • Author(s)
      Kuroda M, Sakaue H.
    • Journal Title

      J Med Invest.

      Volume: 64 Pages: 193-196

    • NAID

      130006105191

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell-cycle arrest in mature adipocytes impairs BAT development but not WAT browning, and reduces adaptive thermogenesis in mice2017

    • Author(s)
      Okamatsu-Ogura Yuko、Fukano Keigo、Tsubota Ayumi、Nio-Kobayashi Junko、Nakamura Kyoko、Morimatsu Masami、Sakaue Hiroshi、Saito Masayuki、Kimura Kazuhiro
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 6648-6648

    • DOI

      10.1038/s41598-017-07206-8

    • NAID

      120006482570

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ligand-induced rapid skeletal muscle atrophy in HSA-Fv2E-PERK transgenic mice.2017

    • Author(s)
      Miyake M, Kuroda M, Kiyonari H, Takehana K, Hisanaga S, Morimoto M, Zhang J, Oyadomari M, Sakaue H, Oyadomari S.
    • Journal Title

      PLoS One.

      Volume: 12 Issue: 6 Pages: e0179955-e0179955

    • DOI

      10.1371/journal.pone.0179955

    • NAID

      120006812009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Endoplasmic reticulum stress in mice increases hepatic expression of genes carrying a premature termination codon via a nutritional status-independent GRP78-dependent mechanism.2017

    • Author(s)
      Harada N, Okuyama M, Yoshikatsu A, Yamamoto H, Ishiwata S, Hamada C, Hirose T, Shono M, Kuroda M, Tsutsumi R, Takeo J, Taketani Y, Nakaya I, Sakaue H.
    • Journal Title

      J Cell Biochem

      Volume: 印刷中 Issue: 11 Pages: 3810-3824

    • DOI

      10.1002/jcb.26031

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Role of Heparin Cofactor Ⅱ in the Regulation of Insulin Sensitivity and Maintenance of Glucose Homeostasis in Humans and Mice2017

    • Author(s)
      Kurahashi K, Inoue S, Yoshida S, Ikeda Y, Morimoto K, Uemoto R, Ishikawa K, Kondo T, Yuasa T, Endo I, Miyake M, Oyadomari S, Matsumoto T, Abe M, Sakaue H, Aihara KI.
    • Journal Title

      Journal of Atherosclerosis and Thrombosis

      Volume: 24 Issue: 12 Pages: 1215-1230

    • DOI

      10.5551/jat.37739

    • NAID

      130006235273

    • ISSN
      1340-3478, 1880-3873
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel lipoprotein (a) lowering drug, D-47, decreases neointima thickening after vascular injury2017

    • Author(s)
      Nakaya Y, Fukuda D, Oyamada T, Ogawa K, Harada N, Nakagami H, Morishita R, Sata M, Sakaue, H.
    • Journal Title

      The Journal of Medical Investigation

      Volume: 64 Issue: 1.2 Pages: 64-67

    • DOI

      10.2152/jmi.64.64

    • NAID

      130005501982

    • ISSN
      1343-1420, 1349-6867
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intracerebroventricular injection of ghrelin decreases wheel running activity in rats.2016

    • Author(s)
      Miyatake Y, Shiuchi T, Mawatari K, Toda S, Taniguchi Y, Futami A, Sato F, Kuroda M, Sebe M, Tsutsumi R, Harada N, Minokoshi Y, Kitamura T, Gotoh K, Ueno M, Nakaya Y, Sakaue H.
    • Journal Title

      Peptide

      Volume: 87 Pages: 12-19

    • DOI

      10.1016/j.peptides.2016.11.005

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-chain monounsaturated fatty acid-rich fish oil attenuates the development of atherosclerosis in mouse models.2016

    • Author(s)
      Yang ZH, Bando M, Sakurai T, Chen Y, Emma-Okon B, Wilhite B, Fukuda D, Pryor BV, Wakabayashi Y, Sampson M, Yu ZX, Sakurai A, Zarzour A, Miyahara H, Takeo J, Sakaue H, Sata M, Remaley AT.
    • Journal Title

      Molecular Nutrition & Food Research

      Volume: 60 Issue: 10 Pages: 2208-2218

    • DOI

      10.1002/mnfr.201600142

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Depot- and gender-specific expression of NLRP3 inflammasome and toll-like receptors in adipose tissue of cancer patients.2016

    • Author(s)
      Shimabukuro M , Sato H, Izaki H, Fukuda D, Uematsu E,Hirata Y, Yagi S, Soeki T, Sakaue H, Kanayama H, Masuzaki H, Sata M
    • Journal Title

      Biofactors.

      Volume: 印刷中 Issue: 4 Pages: 397-406

    • DOI

      10.1002/biof.1287

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Obesity-induced DNA released from adipocytes stimulates chronic adipose tissue2016

    • Author(s)
      Nishimoto S, Fukuda D, Higashikuni Y, Tanaka K, Hirata Y, Murata C, Kim-Kaneyama JR, Sato F, Bando M, Yagi S, Soeki T, Hayashi T, Imoto I, Sakaue H, Shimabukuro M, Sata M.
    • Journal Title

      Sci Adv.

      Volume: 2(3) Issue: 3

    • DOI

      10.1126/sciadv.1501332

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of dietary phosphate on glucose and lipid metabolism.2016

    • Author(s)
      Abuduli M, Ohminami H, Otani T, Kubo H, Ueda H, Kawai Y, Masuda M, Yamanaka-Okumura H, Sakaue H, Yamamoto H, Takeda E, Taketani Y.
    • Journal Title

      Am J Physiol Endocrinol Metab.

      Volume: 301

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] DNA Methylation Suppresses Leptin Gene in 3T3-L1 Adipocytes.2016

    • Author(s)
      Kuroda M, Tominaga A, Nakagawa K, Nishiguchi M, Sebe M, Miyatake Y, Kitamura T, Tsutsumi R, Harada N, Nakaya Y, Sakaue H.
    • Journal Title

      PLoS ONE.

      Volume: 11 Issue: 8 Pages: e0160532-e0160532

    • DOI

      10.1371/journal.pone.0160532

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] PET/CTを用いた骨格筋糖取り込み能の測定2019

    • Author(s)
      三島 優奈, 堤 理恵, 宮武 由実子, 末政 直哉, 大谷 環樹, 兒玉 直子, 丹波 洋介, 黒田 雅士, 升本 早枝子, 阪上 浩
    • Organizer
      第39回日本肥満学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脂肪組織における炎症性サイトカインMCP-1の発現を制御する新たな転写因子の同定2018

    • Author(s)
      黒田 雅士, 石川 悦子, 宇川 菜穂, 川端 康代, 佐々木 和佳, 升本 早枝子, 堤 理恵, 阪上 浩
    • Organizer
      第91回日本内分泌学
    • Related Report
      2018 Annual Research Report
  • [Presentation] 2019120312 脱メチル化3T3-L1脂肪細胞のインスリン感受性調節因子の探索2018

    • Author(s)
      佐々木 和佳, 黒田 雅士, 石川 悦子, 宇川 菜穂, 川端 康代, 升本 早枝子, 堤 理恵, 阪上 浩
    • Organizer
      第39回日本肥満学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 肥大化脂肪細胞における転写因子IRF7の同定と生理的役割の検討2018

    • Author(s)
      川端 康代, 黒田 雅士, 石川 悦子, 宇川 菜穂, 佐々木 和佳, 升本 早枝子, 堤 理恵, 阪上 浩
    • Organizer
      第39回日本肥満学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 肥満脂肪組織病態学の最先端 肥満病態形成におけるIRF7の二つの役割2018

    • Author(s)
      黒田 雅士, 阪上 浩
    • Organizer
      第39回日本肥満学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 栄養から考える肥満・サルコペニア 疾患性サルコペニアにおける栄養 骨格筋分解系に過剰栄養が与える影響2018

    • Author(s)
      堤 理恵, 阪上 浩
    • Organizer
      第39回日本肥満学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 肥大化脂肪細胞における転写因子IRF7の同定と機能解析2018

    • Author(s)
      宇川 菜穂, 黒田 雅士, 石川 悦子, 升本 早枝子, 堤 理恵, 原田 永勝, 阪上 浩
    • Organizer
      第61回日本糖尿病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脂肪組織慢性炎症及びNASH病態形成に対するMFG-E8の関与2018

    • Author(s)
      黒田 雅士, 西口 実佐, 小野山 瑠美, 石川 悦子, 宇川 菜穂, 升本 早枝子, 堤 理恵, 原田 永勝, 阪上 浩
    • Organizer
      第61回日本糖尿病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 謝破綻と疾患 重症患者におけるエネルギー代謝破綻2018

    • Author(s)
      堤 理恵, 阪上 浩
    • Organizer
      第72回日本栄養・食糧学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 肥満脂肪組織病態学-白色・褐色脂肪細胞制御2017

    • Author(s)
      阪上 浩
    • Organizer
      第38回日本肥満学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 肥大化脂肪細胞における転写因子IRF-7の同定と機能解析2017

    • Author(s)
      西口 実佐、阪上 浩
    • Organizer
      第60回日本糖尿病学会年次学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] 肥大化脂肪細胞における転写因子IRF7の同定と機能解析2017

    • Author(s)
      宇川 菜穂、阪上 浩
    • Organizer
      第38回日本肥満学会
    • Related Report
      2017 Research-status Report
  • [Presentation] 肥大化脂肪細胞における転写因子IRF7の同定と機能解析2016

    • Author(s)
      西口 実佐、黒田 雅士、小野山 瑠美、宇川 菜穂、堤 理恵、原田 永勝、阪上 浩
    • Organizer
      第37回日本肥満学会
    • Place of Presentation
      東京ファッションタウンビル(東京都)
    • Year and Date
      2016-10-07
    • Related Report
      2016 Research-status Report
  • [Book] 新時代の臨床糖尿病学【脂肪細胞の分化と機能】2016

    • Author(s)
      黒田 雅士, 阪上 浩
    • Total Pages
      5
    • Publisher
      日本臨床
    • Related Report
      2016 Research-status Report
  • [Book] 実験医学【「解明」から「制御」へ、肥満症のメディカルサイエンス】2016

    • Author(s)
      黒田 雅士, 阪上 浩
    • Total Pages
      7
    • Publisher
      羊土社
    • Related Report
      2016 Research-status Report
  • [Book] Clinical Calcium 【肥満・2型糖尿病におけるビタミンD・カルシウムの役割】2016

    • Author(s)
      黒田 雅士, 阪上 浩
    • Total Pages
      5
    • Publisher
      メディカルトレビューン社
    • Related Report
      2016 Research-status Report
  • [Remarks] 徳島大学大学院 医歯薬学研究部 代謝栄養学分野ホームページ

    • URL

      https://taishaeiyo.jimdo.com

    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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