| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Hippo pathway is an evolutionarily conserved signaling pathway that controls organ size by phosphorylating and inhibiting the transcription co-activators YAP/TAZ in mammals. To elucidate the role of YAP/TAZ in ovarian granulosa cells, the gain-of-function (using the constitutively active mutant) and the loss-of-function analyses (using the siRNA) of YAP/TAZ were performed. Gene expression of steroidogenic enzymes, CYP19A1, CYP11A1 and HSD3B2, were down-regulated by YAP/TAZ. Furthermore, the transcription factor TEAD is involved in YAP/TAZ-mediated these gene expressions. These results suggest that the YAP/TAZ-TEAD nuclear complex is novel negative regulator of steroidogenesis in ovarian granulosa cells.
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