Steroid complement by the steroidogenic cell transplantation
Project/Area Number |
16K09815
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Endocrinology
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Research Institution | Fukuoka University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
柳瀬 敏彦 福岡大学, 医学部, 教授 (30239818)
小玉 正太 福岡大学, 医学部, 教授 (90549338)
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Research Collaborator |
Fukuda Takashi
Muta Yoshimi
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | ステロイドホルモン補充 / 副腎不全 / NR5A1 / 分化誘導 / 移植療法 / ステロイドホルモン / 副腎皮質 / 細胞療法 / 再生医療 / 補充 / 移植・再生医療 / 内分泌学 / 分子生物学 |
Outline of Final Research Achievements |
Hormone replacement therapy by cell transplantation is expected for replacement in response to physiological stimuli.We succeeded in generating steroid-producing cells from mesenchymal cells in vitro, and as a result of transplanting cells to adrenal insufficiency mice, survival time was extended and steroids in blood were detected. The mouse-derived cells did not induce the ACTH receptor by SF-1, and ACTH responsiveness of the transplanted mouse was not observed. Unlike in mice, ACTH responsiveness was observed when human-derived mesenchymal stem cells were used as the source, and in this study, MSCs were generated that turned on SF-1 expression in a drug-induced manner in order to implant them in immunodeficient adrenal insufficiency mice. It has become possible to adjust the required amount of transplanted cells. The hormone replacement effect by transplantation is under investigation.
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Academic Significance and Societal Importance of the Research Achievements |
副腎不全に対するステロイド補充療法は確立された療法であるが、一生涯の補充、生理的な補充は実現できない、副作用等といった問題点がある。細胞療法の利点の一つは、生体内の刺激に応答したホルモン補充である。動物実験で、副腎組織そのものの移植によるホルモン補充効果は報告されている。近年、体性幹細胞、ES細胞、iPS細胞をソースとしたステロイド産生細胞の誘導が細胞培養では成功しているが、これらの細胞を移植し、生体内での補充効果を検討した研究はない。我々は、マウス副腎不全モデルにおいて、ステロイド産生細胞の移植によるホルモン補充効果を証明し、今後の細胞療法開発の基盤となるデータを提供した。
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Clinical Investigation of Adrenal Incidentalomas in Japanese Patients of the Fukuoka Region with Updated Diagnostic Criteria for Sub-clinical Cushing's Syndrome.2018
Author(s)
Abe, I., Sugimoto, K., Miyajima, T., Ide, T., Minezaki, M., Takeshita, K., Takahara, S., Nakagawa, M., Fujimura, Y., Kudo, T., et al.
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Journal Title
Intern Med
Volume: -
Pages: -
DOI
Related Report
Peer Reviewed
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[Journal Article] Selective Androgen Receptor Modulator S42 Suppresses Prostate Cancer Cell Proliferation.2018
Author(s)
Kawanami, T., Tanaka, T., Hamaguchi, Y., Nomiyama, T., Nawata, H., and Yanase, T.
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Journal Title
Endocrinology
Volume: 159
Pages: 1774-1792
DOI
Related Report
Peer Reviewed
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[Journal Article] HMGA1a induces alternative splicing of estrogen receptor alpha in MCF-7 human breast cancer cells.2018
Author(s)
Ohe, K., Miyajima, S., Abe, I., Tanaka, T., Hamaguchi, Y., Harada, Y., Horita, Y., Beppu, Y., Ito, F., Yamasaki, T., et al.
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Journal Title
J Steroid Biochem Mol Biol
Volume: 印刷中
Pages: 印刷中
DOI
Related Report
Peer Reviewed
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[Journal Article] Prevalence of Cardiovascular Disease and Its Risk Factors in Primary Aldosteronism: A Multicenter Study in Japan.2018
Author(s)
Ohno, Y., Sone, M., Inagaki, N., Yamasaki, T., Ogawa, O., Takeda, Y., Kurihara, I., Itoh, H., Umakoshi, H., Tsuiki, M., et al.
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Journal Title
Hypertension
Volume: 71
Pages: 530-537
DOI
Related Report
Peer Reviewed
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[Journal Article] Accuracy of adrenal computed tomography in predicting the unilateral subtype in young patients with hypokalaemia and elevation of aldosterone in primary aldosteronism.2018
Author(s)
Umakoshi, H., Ogasawara, T., Takeda, Y., Kurihara, I., Itoh, H., Katabami, T., Ichijo, T., Wada, N., Shibayama, Y., Yoshimoto, T., et al.
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Journal Title
Clin Endocrinol (Oxf)
Volume: 88
Pages: 645-651
DOI
Related Report
Peer Reviewed
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[Journal Article] Significance of Computed Tomography and Serum Potassium in Predicting Subtype Diagnosis of Primary Aldosteronism.2018
Author(s)
Umakoshi, H., Tsuiki, M., Takeda, Y., Kurihara, I., Itoh, H., Katabami, T., Ichijo, T., Wada, N., Yoshimoto, T., Ogawa, Y., et al.
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Journal Title
J Clin Endocrinol Metab
Volume: 103
Pages: 900-908
DOI
Related Report
Peer Reviewed
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[Journal Article] Low glycated hemoglobin level is associated with severity of frailty in Japanese elderly diabetes patients.2018
Author(s)
Yanagita, I., Fujihara, Y., Eda, T., Tajima, M., Yonemura, K., Kawajiri, T., Yamaguchi, N., Asakawa, H., Nei, Y., Kayashima, Y., et al.
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Journal Title
J Diabetes Investig
Volume: 9
Pages: 419-425
DOI
Related Report
Peer Reviewed
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[Journal Article] New diagnostic criteria of adrenal subclinical Cushing's syndrome: opinion from the Japan Endocrine Society.2018
Author(s)
Yanase, T., Oki, Y., Katabami, T., Otsuki, M., Kageyama, K., Tanaka, T., Kawate, H., Tanabe, M., Doi, M., Akehi, Y., et al.
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Journal Title
Endocr J
Volume: 65
Pages: 383-393
DOI
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Peer Reviewed
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[Journal Article] Postpartum hypothalamic adrenal insufficiency with remission: A rare case2017
Author(s)
Akehi, Y., Hashimoto, Y., Meren, J., Tanabe, M., Nomiyama, T., and Yanase, T.
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Journal Title
Endocr J
Volume: 64
Pages: 157-162
DOI
Related Report
Peer Reviewed
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[Journal Article] A higher score on the Aging Males' Symptoms scale is associated with insulin resistance in middle-aged men.2017
Author(s)
Hamanoue, N., Tanabe, M., Tanaka, T., Akehi, Y., Murakami, J., Nomiyama, T., and Yanase, T.
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Journal Title
Endocr J
Volume: 64
Pages: 521-530
DOI
Related Report
Peer Reviewed
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[Journal Article] Hepatectomy for Liver Metastases of Colorectal Cancer After Adoptive Chemoimmunotherapy Using Activated alphabeta T-cells.2017
Author(s)
Ishii, F., Yoshida, Y., Yamauchi, Y., Aisu, N., Kojima, D., Mera, T., Kato, D., Tanaka, T., Naito, K., Yasumoto, K., et al.
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Journal Title
Anticancer Res
Volume: 37
Pages: 3933-3939
DOI
Related Report
Peer Reviewed
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[Journal Article] Exendin-4, a Glucagonlike Peptide-1 Receptor Agonist, Attenuates Breast Cancer Growth by Inhibiting NF-kappaB Activation.2017
Author(s)
Iwaya, C., Nomiyama, T., Komatsu, S., Kawanami, T., Tsutsumi, Y., Hamaguchi, Y., Horikawa, T., Yoshinaga, Y., Yamashita, S., Tanaka, T., et al.
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Journal Title
Endocrinology
Volume: 158
Pages: 4218-4232
DOI
Related Report
Peer Reviewed
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[Journal Article] CD44 Promotes Inflammation and Extracellular Matrix Production During Arteriovenous Fistula Maturation.2017
Author(s)
Kuwahara, G., Hashimoto, T., Tsuneki, M., Yamamoto, K., Assi, R., Foster, T.R., Hanisch, J.J., Bai, H., Hu, H., Protack, C.D., et al.
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Journal Title
Arterioscler Thromb Vasc Biol
Volume: 37
Pages: 1147-1156
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Syndrome of inappropriate antidiuretic hormone secretion in a case of olfactory neuroblastoma without anti-diuretic hormone immunoreactivity: A case report and review of the literature.2017
Author(s)
Nakano, T., Motoshita, J., Sawada, F., Okabe, M., Tamae, A., Hiramatsu, S., Kodama, S., Shiratsuchi, H., Yasumatsu, R., Nakashima, T., et al.
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Journal Title
Auris Nasus Larynx
Volume: 44
Pages: 771-774
DOI
Related Report
Peer Reviewed
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[Journal Article] Prolonged Inflation Technique Using a Scoring Balloon for Severe Calcified Lesion.2017
Author(s)
Otsuka, Y., Koyama, T., Imoto, Y., Katsuki, Y., Kawahara, M., Nakamura, K., Kodama, S., Noguchi, H., and Iwasaki, K.
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Journal Title
Int Heart J
Volume: 58
Pages: 082-987
DOI
Related Report
Peer Reviewed
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[Journal Article] A Modified Adenovirus Vector-Mediated Antibody Screening Method Identifies EphA2 as a Cancer Target.2017
Author(s)
Tanaka, T., Yamada, H., Kuroki, M., Kodama, S., Tamura, K., and Takamatsu, Y.
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Journal Title
Transl Oncol
Volume: 10
Pages: 476-484
DOI
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Peer Reviewed
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[Journal Article] Clinical Study on the Medical Value of Combination Therapy Involving Adoptive Immunotherapy and Chemotherapy for Stage IV Colorectal Cancer (COMVI Study).2017
Author(s)
Yoshida, Y., Naito, M., Yamada, T., Aisu, N., Kojima, D., Mera, T., Tanaka, T., Naito, K., Yasumoto, K., Kamigaki, T., et al.
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Journal Title
Anticancer Res
Volume: 37
Pages: 3941-3946
DOI
Related Report
Peer Reviewed
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