Molecular pathogenesis of familial myelodysplastic syndromes

• Project/Area Number: 16K09831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Tokyo Metropolitan Komagome Hospital (Clinical research laboratory) (2018); Bunkyo Gakuin University (2016-2017)
• Principal Investigator: HARADA Yuka 東京都立駒込病院(臨床研究室), 臨床試験科, 医長 (50379848)
• Co-Investigator(Kenkyū-buntansha): 原田 浩徳 東京薬科大学, 生命科学部, 教授 (10314775)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 家族性造血器腫瘍 / 骨髄異形成症候群（MDS） / ターゲットシークエンス / RUNX1変異 / TP53変異 / マウス骨髄移植モデル / マウスBMTモデル / 癌

Outline of Final Research Achievements

Germline predisposition is increasingly recognized in myelodysplastic syndromes (MDS). We found 20 pedigrees of myeloid neoplasms and analyzed the mutations of RUNX1, CEBPA, GATA2 and DDX41 genes. Using Sanger sequencing and target sequencing by next generation sequencing, only 6 pedigrees were identified their responsible gene mutations. The responsible gene mutations could not be identified in other 14 pedigrees, suggesting that there still may exist many potential pedigrees of familial myeloid neoplasms caused by unknown gene mutations.
We identified a collaboration partner gene, Gene Y, which was detected in iPS cells from RUNX1-mutated pedigree. Co-transduction of a RUNX1-mutant and Gene Y in mouse bone marrow transplantation model developed MDS with severe anemia in a few months. Therefore, we suspected that a RUNX1 mutation is one of the stem cell aging factors.

Academic Significance and Societal Importance of the Research Achievements

家族性造血器腫瘍20家系のうち、既知の原因遺伝子のうちいずれかの変異を認めた家系は少数にとどまり、大半の症例で原因遺伝子変異が同定されなかったことから、germline変異の原因遺伝子変異として知られているものは一部に過ぎず、潜在的に多くの家系が未知の遺伝子異常により家族性造血器腫瘍を発症していることが示唆された。また、RUNX1変異自体が分化障害を有し、Gene Yと協調して非常に短期間でMDSを発症することから、RUNX1変異自体がエイジング因子であると考えられた。

Research Products

• [Journal Article] Prominence of nestin-expressing Schwann cells in bone marrow of patients with myelodysplastic syndromes with severe fibrosis (2019)
  • Author(s): Cao-Sy Luan、Obara Naoshi、Sakamoto Tatsuhiro、Kato Takayasu、Hattori Keiichiro、Sakashita Shingo、Nannya Yasuhito、Ogawa Seishi、Harada Hironori、Sakata-Yanagimoto Mamiko、Nishikii Hidekazu、Chiba Shigeru
  • Journal Title: International Journal of Hematology Volume: 109 Issue: 3 Pages: 309-318
  • DOI: 10.1007/s12185-018-02576-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A germline HLTF mutation in familial MDS induces DNA damage accumulation through impaired PCNA polyubiquitination (2019)
  • Author(s): Takaoka Kensuke、Kawazu Masahito、Koya Junji、Yoshimi Akihide、Masamoto Yosuke、Maki Hiroaki、Toya Takashi、Kobayashi Takashi、Nannya Yasuhito、Arai Shunya、Ueno Toshihide、Ueno Hironori、Suzuki Kenshi、Harada Hironori、Manabe Atsushi、Hayashi Yasuhide、Mano Hiroyuki、Kurokawa Mineo
  • Journal Title: Leukemia Volume: 印刷中 Issue: 7 Pages: 1773-1782
  • DOI: 10.1038/s41375-019-0385-0
  • Peer Reviewed
• [Journal Article] Hypoxia/pseudohypoxia‐mediated activation of hypoxia‐inducible factor‐1α in cancer (2019)
  • Author(s): Hayashi Yoshihiro、Yokota Asumi、Harada Hironori、Huang Gang
  • Journal Title: Cancer Science Volume: 110 Issue: 5 Pages: 1510-1517
  • DOI: 10.1111/cas.13990
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 急性白血病の分子病態 (2019)
  • Author(s): 原田結花，原田浩徳
  • Journal Title: 医学のあゆみ Volume: 268 Pages: 10-16
• [Journal Article] Pathobiologic Pseudohypoxia as a Putative Mechanism Underlying Myelodysplastic Syndromes. (2018)
  • Author(s): Hayashi Y, Zhang Y, Yokota A, Yan X, Liu J, Choi K, Li B, Sashida G, et al.
  • Journal Title: Cancer Discovery Volume: 8 Issue: 11 Pages: 1438-57
  • DOI: 10.1158/2159-8290.cd-17-1203
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Impact of splicing factor mutations on clinical features in patients with myelodysplastic syndromes (2018)
  • Author(s): Shingai Naoki、Harada Yuka、Iizuka Hiroko、Ogata Yosuke、Doki Noriko、Ohashi Kazuteru、Hagihara Masao、Komatsu Norio、Harada Hironori
  • Journal Title: International Journal of Hematology Volume: 108 Issue: 6 Pages: 598-606
  • DOI: 10.1007/s12185-018-2551-y
  • Peer Reviewed / Open Access
• [Journal Article] High expression of ABCG2 induced by EZH2 disruption has pivotal roles in MDS pathogenesis (2017)
  • Author(s): Kawabata K C、Hayashi Y、Inoue D、Meguro H、Sakurai H、Fukuyama T、Tanaka Y、Asada S、Fukushima T、Nagase R、Takeda R、Harada Y、Kitaura J、Goyama S、Harada H、Aburatani H、Kitamura T
  • Journal Title: Leukemia Volume: 32 Issue: 2 Pages: 419-428
  • DOI: 10.1038/leu.2017.227
  • Peer Reviewed / Open Access
• [Journal Article] Overexpression of RUNX1 short isoform has an important role in the development of myelodysplastic/myeloproliferative neoplasms (2017)
  • Author(s): Sakurai Hiroko、Harada Yuka、Ogata Yosuke、Kagiyama Yuki、Shingai Naoki、Doki Noriko、Ohashi Kazuteru、Kitamura Toshio、Komatsu Norio、Harada Hironori
  • Journal Title: Blood Advances Volume: 1 Issue: 18 Pages: 1382-1386
  • DOI: 10.1182/bloodadvances.2016002725
  • Peer Reviewed / Open Access
• [Journal Article] Myeloid neoplasms with germ line RUNX1 mutation (2017)
  • Author(s): Hayashi Yoshihiro、Harada Yuka、Huang Gang、Harada Hironori
  • Journal Title: International Journal of Hematology Volume: 106 Issue: 2 Pages: 183-188
  • DOI: 10.1007/s12185-017-2258-5
  • Peer Reviewed / Open Access
• [Journal Article] Guest Editorial: Understanding of MPN and MDS/MPN based on molecular pathogenesis and clinical aspects (2017)
  • Author(s): Harada Hironori
  • Journal Title: International Journal of Hematology Volume: 105 Issue: 6 Pages: 709-710
  • DOI: 10.1007/s12185-017-2244-y
  • Peer Reviewed / Open Access
• [Journal Article] Donor cell leukemia arising from preleukemic clones with a novel germline DDX41 mutation after allogenic hematopoietic stem cell transplantation (2017)
  • Author(s): Kobayashi S、Kobayashi A、Osawa Y、Nagao S、Takano K、Okada Y、Tachi N、Teramoto M、Kawamura T、Horiuchi T、Kato S、Maekawa T、Yamamura T、Watanabe J、Harada Y、Harada H、Sato K、Kimura F
  • Journal Title: Leukemia Volume: 31 Issue: 4 Pages: 1020-1022
  • DOI: 10.1038/leu.2017.44
  • Peer Reviewed
• [Journal Article] MDS分子病態研究の進歩 (2017)
  • Author(s): 原田浩徳
  • Journal Title: 臨床血液 Volume: 58 Pages: 1941-1950
  • NAID: 130006132680
  • Peer Reviewed
• [Journal Article] 非5q欠失骨髄異形成症候群に対するレナリドミドの臨床効果 (2017)
  • Author(s): 原田結花、原田浩徳
  • Journal Title: 血液内科 Volume: 74 Pages: 644-649
• [Journal Article] レナリドミド（低リスクMDS）［骨髄疾患による貧血：成人骨髄異形成症候群（MDS）治療］ (2017)
  • Author(s): 原田結花，原田浩徳
  • Journal Title: 日本臨牀増刊号「貧血学」 Volume: 75(Suppl 1) Pages: 262-267
• [Journal Article] Spectrum of clinical and genetic features of patients with inherited platelet disorder with suspected predisposition to hematological malignancies: a nationwide survey in Japan. (2016)
  • Author(s): Yoshimi A, Toya T, Nannya Y, Takaoka K, Kirito K, Ito E, Nakajima H, Hayashi Y, Takahashi T, moriya-Saito A, Suzuki K, Harada H, Komatsu N, Usuki K, Ixhikawa M, Kurokawa M.
  • Journal Title: Annals of Oncology Volume: 27 Issue: 5 Pages: 887-895
  • DOI: 10.1093/annonc/mdw066
  • Peer Reviewed / Open Access
• [Journal Article] The loss of Ezh2 cooperates with an active JAK2 mutant in the pathogenesis of myelofibrosis and sensitizes tumor-initiating cells to bromodomain inhibition. (2016)
  • Author(s): Sashida G, Wang S, Tomioka T, Oshima M, Kazumasa Aoyama K, Kanai A, Mochizuki-Kashio M, Harada H, Shimoda K, Iwama A.
  • Journal Title: J Experimenta l Medicine Volume: 213 Issue: 8 Pages: 1459-1477
  • DOI: 10.1084/jem.20151121
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 【骨髄増殖性腫瘍のマネジメントと最近の展開】 原発性骨髄線維症におけるオーロラキナーゼの作用機序. (2016)
  • Author(s): 新谷直樹，原田結花，原田浩徳
  • Journal Title: 血液内科 Volume: 73(3) Pages: 310-5
• [Journal Article] del(5q)MDSに対するレナリドミドの作用機構 (2016)
  • Author(s): 原田結花，原田浩徳
  • Journal Title: 血液内科 Volume: 72(5) Pages: 665-670
  • NAID: 40020870119
• [Journal Article] エピゲノム変異による白血病発症［白血病の発症機序］ (2016)
  • Author(s): 新谷直樹，原田結花，原田浩徳
  • Journal Title: 日本臨牀増刊号「白血病学（上）」 Volume: 74(Suppl 8) Pages: 345-349
• [Journal Article] アルキル化剤［白血病の発症機序：抗がん剤による治療関連白血病の発症機構］ (2016)
  • Author(s): 原田結花，原田浩徳
  • Journal Title: 日本臨牀増刊号「白血病学（上）」 Volume: 74(Suppl 8) Pages: 350-354
• [Presentation] MDS with fibrosisの臨床および分子生物学的病態解明 (2019)
  • Author(s): 林嘉宏，新谷直樹，鍵山侑希，原田結花，原田浩徳
  • Organizer: 第23回造血器腫瘍研究会
• [Presentation] Prognostic value of TP53 mutation and monosomal karyotype for AML in non-remission at allo-HCT (2019)
  • Author(s): 14.Najima Y, Sadato D, Hirama C, Harada Y, Oboki K, Toya T, Doki N, Haraguchi K, Adachi H, Konuma R, Fujita M, Wada A, Kishida Y, Konishi T, Nagata A, Yamada Y, Kaito S, Yoshifuji K, Akiyama M, Inamoto K, Igarashi A, Kobayashi T, Kakihana K, Okuyama Y, Sakamaki H, Harada H, Ohashi K
  • Organizer: 第41回日本造血細胞移植学会総会
• [Presentation] NUP98-HBO1 Induces Phenotypically and Genetically Relevant Chronic Myelomonocytic Leukemia Pathogenesis through Aberrant Histone Acetylation. (2018)
  • Author(s): Hayashi Y, Harada Y, Matsui H, Kato N, Ding Y, Kagiyama Y, Kitabayashi I, Iwama A, Kitamura T, Harada H
  • Organizer: 12th International Workshop on Molecular Aspects On Myeloid Stem Cell Development And Leukemia
  • Int'l Joint Research
• [Presentation] NUP98-HBO1 Induces Clinically Relevant Chronic Myelomonocytic Leukemia Pathogenesis through Aberrant Histone Acetylation (2018)
  • Author(s): Harada Y, Hayashi Y, Kagiyama Y, Matsui H, Kitabayashi I, Iwama A, Kitamura T, Harada H
  • Organizer: ISEH 46th Annual Scientific Meeting
  • Int'l Joint Research
• [Presentation] Aberrant Histone Acetylation by HBO-1 fusion Generates Clinically Relevant Chronic Myelomonocytic Leukemia Pathogenesis (2018)
  • Author(s): Hayashi Y, Harada Y, Kagiyama Y, Shingai N, Komatsu N, Matsui H, Kitabayashi I, Iwama A, Kitamura T, Harada H
  • Organizer: 第77回日本癌学会学術総会
• [Presentation] NUP98-HBO1 Induces Clinically Relevant CMML Pathogenesis through Aberrant Histone Acetylation (2018)
  • Author(s): Hayashi Y, Harada Y, Kagiyama Y, Matsui H, Kato N, Ding Y, Imagawa J, Kitaura J, Kitabayashi I, Iwama A, Kitamura T, Harada H
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] 骨髄線維化を伴う骨髄異形成症候群の臨床病態と分子発症機序の解明 (2018)
  • Author(s): 新谷直樹，原田浩徳，原田結花，飯塚弘子，森下総司，荒木真理人，大阪顯通，土岐典子，大橋一輝，萩原政夫，小松則夫
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] 慢性骨髄単球性白血病の臨床病態に関わる遺伝子異常の解析 (2018)
  • Author(s): 滝口慎太郎，新谷直樹，下村莉子，尾形洋輔，土岐典子，大橋一輝，萩原政夫，小松則夫，原田結花，原田浩徳
  • Organizer: 第80回日本血液学会学術集会
• [Presentation] Presence of TP53 mutation and monosomal karyotype predict the outcome of patients with acute myeloid leukemia in non-remission at allogeneic stem cell transplantation (2018)
  • Author(s): 9.Najima Y, Oboki K, Harada Y, Sadato D, Hirama C, Toya T, Doki N, Haraguchi K, Kobayashi T, Kakihana K, Inamoto K, Okuyama Y, Sakamaki H, Harada H, Ohashi K
  • Organizer: 60th ASH Annual Meeting and Exposition
  • Int'l Joint Research
• [Presentation] ヒストンアセチル化制御破綻による慢性骨髄単球性白血病の新規発症機序の解明 (2018)
  • Author(s): 林嘉宏，原田結花，丁曄，松井啓隆，鍵山侑希，加藤菜穂子，北林一生，岩間厚志，北村俊雄，原田浩徳
  • Organizer: 第22回造血器腫瘍研究会
• [Presentation] 家族性MDSにおけるHLTF変異によりPCNAのポリユビキチン化が損なわれDNA損傷が蓄積する (2018)
  • Author(s): 高岡賢輔，河津正人，古屋淳史，吉見昭秀，牧宏彰，遠矢嵩，小林隆，南谷泰仁，荒井俊也，上野博則，鈴木憲史，原田浩徳，真部淳，林泰秀，間野博行，黒川峰夫
  • Organizer: 第22回造血器腫瘍研究会
• [Presentation] 造血器腫瘍における抗がん剤、G-CSF製剤が与える影響の解明 (2017)
  • Author(s): 新谷直樹，西尾美和子，原田結花，原田浩徳
  • Organizer: 第21回造血器腫瘍研究会
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2017-02-17
• [Presentation] RUNX3スーパーエンハンサーによる白血病幹細胞の病態基盤解明 (2017)
  • Author(s): 横溝貴子，大島基彦，原田浩徳，岩間厚志，大里元美，指田吾郎
  • Organizer: 第21回造血器腫瘍研究会
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2017-02-17
• [Presentation] Molecular mechanisms to development myeloid neoplasms by RUNX1 or MLL chimeras in human CD34+ cells (2017)
  • Author(s): Harada H, Shingai N, Nishio M, Komatsu N, Harada Y
  • Organizer: ISEH 46th Annual Scientific Meeting
  • Int'l Joint Research
• [Presentation] 骨髄異形成症候群およびその類縁疾患における骨髄線維化機序の解明 (2017)
  • Author(s): 新谷直樹，原田浩徳，原田結花，荒木真理人，小松則夫
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] 家族性骨髄異形成症候群から得られたHLTF変異によりPCNAのポリユビキチン化が損なわれDNA損傷が蓄積する (2017)
  • Author(s): 高岡賢輔，河津正人，古屋淳史，吉見昭秀，牧宏彰，遠矢嵩，南谷泰仁，荒井俊也，上野博則，原田浩徳，林泰秀，間野博行，黒川峰夫
  • Organizer: 第76回日本癌学会学術総会
• [Presentation] MDS分子病態研究の進歩 (2017)
  • Author(s): 原田浩徳
  • Organizer: 第79回日本血液学会学術集会
  • Invited
• [Presentation] HIF1Aシグナルの亢進は骨髄異形成症候群の病態形成において中心的な役割を果たす (2017)
  • Author(s): 林嘉宏, Zhang Y, Yan Z, 指田吾郎, Chetai K, Olsson A, 原田浩徳, Shih L-Y, Tse W, Bridges J, Zheng Y, Witte D, Caligiuri M, Qu C-K, Whang Q-F, Salomonis N, Grimes HL, Nimer S, Xiao Z, Huang G
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] 家族性MDSにおけるHLTF変異によりPCNAのポリユビキチン化が損なわれDNA損傷が蓄積する (2017)
  • Author(s): 高岡賢輔，河津正人，古屋淳史，吉見昭秀，牧宏彰，遠矢嵩，小林隆，南谷泰仁，荒井俊也，上野博則，鈴木憲史，原田浩徳，真部淳，林泰秀，間野博行，黒川峰夫
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] スプライシング関連遺伝子異常が運命づけるMDS表現型の解析 (2017)
  • Author(s): 新谷直樹，原田結花，櫻井弘子，尾形洋輔，赤羽浩太，西尾美和子，土岐典子，大橋一輝，萩原政夫，小松則夫，原田浩徳
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] RUNX3スーパーエンハンサーによる白血病幹細胞の病態基盤解明 (2017)
  • Author(s): 横溝貴子，田中大樹，原田結花，大島基彦，金井昭教，岩間厚志，大里元美，原田浩徳，指田吾郎
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] 環状鉄芽球を伴う骨髄異形成症候群におけるスプライシング関連遺伝子異常 (2017)
  • Author(s): 尾形洋輔，新谷直樹，赤羽浩太，西尾美和子，土岐典子，香西康司，萩原政夫，大橋一輝，小松則夫，原田結花，原田浩徳
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] 急性骨髄性白血病に対するターゲットシーケンスパネル (2017)
  • Author(s): 松下弘道，川井英嗣，田中政之，原田浩徳，原田結花，天木惇，盛音，椎名隆，鬼塚真仁，安藤潔
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] ポリコーム遺伝子Pcgf1の欠損は、JAK2V617Fによる骨髄線維症の発症を著明に促進する (2017)
  • Author(s): 篠田大輔，中島やえ子，大島基彦，更屋敦則，指田吾郎，原田浩徳，下田和哉，古関明彦，岩間厚志
  • Organizer: 第79回日本血液学会学術集会
• [Presentation] Identification of critical mediator for bone marrow fibrosis in MDS using novel RUNX1-mutatant/HMGA2-overexpression mouse model (2017)
  • Author(s): Harada H, Hayashi Y, Shingai N, Kagiyama Y, Harada Y
  • Organizer: The 21st International RUNX Conference
  • Int'l Joint Research
• [Presentation] XPO1 inhibition targets transcriptional vulnerability of FLT3-ITD+D835 double mutant AML through p53 accumulation and inhibition of oncogenic transcription factors: Lesson learned from CAGE sequencing of primary AML cells (2017)
  • Author(s): Yamatani K, Tabe Y, Sekihara K, Yang H, Saitoh K, Zhang W, Ikeo K, Kinjo S, Mogushi K, Hosoya M, Hayashizaki Y, Yamanaka Y, Harada H, Miida T, Konopleva M, Andreeff M
  • Organizer: 59th ASH Annual Meeting and Exposition
  • Int'l Joint Research
• [Presentation] RUNX3 promotes the development of MDS/MPN Overlap Syndrome via enhancing expression of Myc in the absence of Tet2 (2017)
  • Author(s): Yokomizo T, Tanaka D, Kubota S, Oshima M, Harada Y, Kanai A, Iwama A, Harada H, Osato M, Sashida G
  • Organizer: 59th ASH Annual Meeting and Exposition
  • Int'l Joint Research
• [Presentation] Human Germline HLTF E259K Mutation Identified in Familial MDS Patients Accumulates DNA Damage through Impaired PCNA Polyubiquitination (2017)
  • Author(s): Takaoka K, Kawazu M, Koya J, Yoshimi A, Masamoto Y, Maki H, Toya T, Kobayashi T, Nannya Y, Arai S, Ueno H, Suzuki K, Harada H, Manabe A, Hayashi Y, Mano H, Kurokawa M
  • Organizer: 59th ASH Annual Meeting and Exposition
  • Int'l Joint Research
• [Presentation] 家族性骨髄異形成症候群の全国調査 (2017)
  • Author(s): 黒川峰夫，古屋淳史，高岡賢輔，遠矢 嵩，小林 隆，原田浩徳，真部 淳，林 泰秀
  • Organizer: 平成28年度厚生労働科学研究費補助金 難治性疾患等政策研究事業 特発性造血器障害に関する調査研究班会議
  • Place of Presentation: 東京大学（東京都文京区）
• [Presentation] 遺伝子異常による骨髄系造血器腫瘍の発症機序解明 (2017)
  • Author(s): 原田浩徳
  • Organizer: 東京大学医科学研究所共同研究拠点事業 平成28年度成果報告会
  • Place of Presentation: 東京大学医科学研究所（東京都港区）
• [Presentation] Disordered Gene Expression in Hematologic Malignancy,including Disordered Epigenetic Regulation (2016)
  • Author(s): Tabe Y, Sekihara K, Saitoh K, Monma N, Ikeo K, Kaczkowski B, Zhang W, Yamanaka Y, Miida T, Shah N, Harada H, Hayashizaki Y, Konopleva M, Andreeff M
  • Organizer: 58th ASH Annual Meeting and Exposition
  • Place of Presentation: SanDiego, CA (USA)
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Nationwide epidemiological surver of familial myelodysplastic syndromes/acute myeloid leukemia in Japan (2016)
  • Author(s): Takaoka K, Yoshimi A, Koya J, Toya T, Kobayashi T, Nannya Y, Ueno H, Suzuki K, Harada H, Manabe A, Hayashi Y, Kurokawa M
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
• [Presentation] Analysis of Asxl1-MT conditional knock-in mice (2016)
  • Author(s): Nagase R, Inoue D, Kanai A, Saika M, Fujino T, Kawabata K, Tanaka Y, Fukuyama T, Harada H, Goyama S, Honda H, Kitamura T
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
• [Presentation] Expansion of ABCG2 is regulated in EZH2-related MDS and associated with its pathogenesis (2016)
  • Author(s): Kawabata K, Hayashi Y, Inoue D, Sakurai H, Mizuno H, Kitaura J, Harada Y, Harada H, Goyama S, Aburatani H, Ishii M, Kitamura T
  • Organizer: 第78回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-13
• [Presentation] Molecular mechanisms to develop myeloid neoplasms by RUNX1 or MLL chimeras in human CD34 cells (2016)
  • Author(s): Shingai N, Harada Y, Nishio M, Harada H
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] ABCG2 High Expression Is Specific to Advanced MDS and Promotes Cytopenia in Mouse BMT Model (2016)
  • Author(s): Kawabata K, Hayashi Y, Inoue D, Kitaura J, Goyama S, Harada Y, Harada H, Aburatani H, Kitamura T
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] Nationwide epidemiological surver of familial myelodysplastic syndromes/acute myeloid leukemia (2016)
  • Author(s): Takaoka K, Yoshimi A, Koya J, Toya T, Kobayashi T, Nannya Y, Ueno H, Harada H, Hayashi Y, Kurokawa M
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 家族性骨髄異形成症候群/急性骨髄性白血病の本邦における疫学 (2016)
  • Author(s): Takaoka K, Yoshimi A, Koya J, Kobayashi T, Ueno H, Suzuki K, Harada H, Manabe A, Hayashi Y, Kurokawa M
  • Organizer: 第14回日本臨床腫瘍学会学術集会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-07-28
• [Presentation] ysregulation of RUNX1 Plays a Critical Role in the Progression of Myelodysplastic Syndromes (2016)
  • Author(s): Harada H, Harada Y, Sakurai H, Kitamura T, Komatsu N
  • Organizer: The eleventh international workshop on molecular aspects of myeloid stem cell development and leukemia
  • Place of Presentation: Cincinnati Children's Hospital Medical Center, Cincinnati (Ohio, USA)
  • Year and Date: 2016-05-05
  • Int'l Joint Research
• [Presentation] 骨髄異形成症候群の基礎知識 (2016)
  • Author(s): 原田浩徳
  • Organizer: 第64回日本輸血・細胞治療学会総会
  • Place of Presentation: 国立京都国際会館（京都府京都市）
  • Year and Date: 2016-04-28
  • Invited
• [Presentation] 骨髄異形成症候群の分子病態と治療 (2016)
  • Author(s): 原田浩徳
  • Organizer: 第34回日本血液学会北陸地方会
  • Place of Presentation: 石川県立中央病院（石川県金沢市）
  • Invited
• [Book] 単球増加．内科外来診断navi，富野康日己監修 (2017)
  • Author(s): 原田浩徳
  • Publisher: 中外医学社
• [Book] 顆粒球減少症．研修ノートシリーズ「血液科研修ノート」，神田善伸責任編集 (2016)
  • Author(s): 原田浩徳
  • Publisher: 診断と治療社
• [Book] アルキル化薬による染色体異常とMDSの発生．造血器腫瘍アトラス改訂第５版，阿部達生編 (2016)
  • Author(s): 原田結花，原田浩徳
  • Publisher: 日本医事新報社
• [Book] Epigenetic changesと白血病―CpGアイランドのメチル化やヒストン脱アセチル化．造血器腫瘍アトラス改訂第５版，阿部達生編 (2016)
  • Author(s): 原田結花，原田浩徳
  • Publisher: 日本医事新報社