Pathophysiological analysis of thrombosis using genetically engineered mouse models
| Project/Area Number |
16K09835
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Japan Women's University (2018)
Koriyama Women's University (2016-2017) |
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Principal Investigator |
BANNO FUMIAKI 日本女子大学, 家政学部, 講師 (00373514)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 血栓症モデルマウス / プロテインS / プラスミノーゲン / ADAMTS13 / インテグリン / 血小板 / 炎症 / 静脈血栓症 / 遺伝子 / 生体分子 / 動物 |
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| Outline of Final Research Achievements |
In this study, we analyzed the effects of thrombotic molecules and their gene mutations on the pathogenesis of thrombosis using genetically modified mice established so far. The coexistence of the plasminogen-A622T mutation did not aggravate the thrombotic tendency caused by the protein S-K196E mutation. ADAMTS13 suppressed excessive neutrophil infiltration in the innate immune response. Extracellular signal-regulated kinase in platelets was essential for initiation and maintenance of thrombus formation. Protein kinase A in platelets inhibited thrombus formation only in the early phase. The integrin αIIb-R990W mutation induced constitutive activation of αIIbβ3 on platelets, but simultaneously reduced the expression of αIIbβ3 to cause impaired platelet aggregation.
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| Academic Significance and Societal Importance of the Research Achievements |
血栓症に関わる分子やその遺伝子変異の病態生理学的意義が明らかとなり、血栓症治療の充実につながる成果が得られた。
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] ADAMTS-13 regulates neutrophil recruitment in a mouse model of invasive pulmonary aspergillosis2017
Author(s)
Hasibeder A, Prufer S, Ebner K, Reuter S, Lopez PA, Scharrer I, Banno F, Stassen M, Schild H, Jurk K, Bosmann M, Beckert H, Radsak MP
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 7184-7184
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Live imaging of extracellular signal-regulated kinase and protein kinase A activities during thrombus formation in mice expressing biosensors based on Forster resonance energy transfer2018
Author(s)
Matsuda M, Hiratsuka T, Sano T, Kato H, Komatsu N, Imajo M, Kamioka Y, Sumiyama K, Banno F, Miyata T
Organizer
第40回日本血栓止血学会学術集会, SPCシンポジウム2
Related Report
Invited
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[Presentation] インテグリンαIIbβ3活性化変異αIIb (R990W) ノックインマウスにおける血小板産生障害2018
Author(s)
芥田敬吾, 清水一亘, 柏木浩和, 國島伸治, 坂野史明, 小亀浩市, 西浦伸子, 森川陽一郎, 加藤恒, 本田繁則, 金倉譲, 宮田敏行, 冨山佳昭
Organizer
第40回日本血栓止血学会学術集会
Related Report
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[Presentation] αIIb (R990W) knock-in mice show impaired thrombopoiesis2018
Author(s)
Akuta K, Kiyomizu K, Kashiwagi H, Kunishima S, Banno F, Kokame K, Nishiura N, Morikawa Y, Kato H, Honda S, Kanakura Y, Miyata T, Tomiyama Y
Organizer
第80回日本血液学会学術集会
Related Report
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[Presentation] ADAMTS-13 regulates neutrophil recruitment in a mouse model of invasive pulmonary aspergillosis2017
Author(s)
Hasibeder A, Prufer S, Ebner K, Reuter S, Lopez PA, Scharrer I, Banno F, Stassen M, Schild H, Jurk K, Bosmann M, Beckert H, Radsak MP
Organizer
22nd Congress of European Hematology Association
Related Report
Int'l Joint Research
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