| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Human CD16-positive monocytes had high ability to secrete active form of IL-1β. Secretion of active IL-1β from peripheral blood monocytes after LPS stimulation was higher in rheumatoid arthritis (RA) with high disease activity compared to healthy controls, because CD16 positive monocytes were increased in active RA. The suppressive reagents for IL-1 β secretion was examined. Colchicine inhibited IL-1β secretion from LPS-stimulated monocytes at high concentrations. Hydroxychloroquine also suppressed IL-1β secretion from LPS stimulated monocytes. There was no extracellular release of the cytoplasmic components of monocytes and no cell death was observed. Therefore, it was considered that the secretion of active IL-1β from monocytes was mediated by a different pathway from pyroptosis in macrophages.
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