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Development of a new vaccine against human coronavirus causing severe pneumonia

Research Project

Project/Area Number 16K09951
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Infectious disease medicine
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

Iwata-Yoshikawa Naoko  国立感染症研究所, 感染病理部, 主任研究官 (10360695)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsワクチン / 重症肺炎 / コロナウイルス / 感染症防御 / 新規ワクチン / ヒトコロナウイルス
Outline of Final Research Achievements

The spike (S) protein of coronavirus is a candidate vaccine target for blocking coronavirus infection. However, some animal studies have suggested that inadequate immunization against SARS‐CoV induces a lung eosinophilic immunopathology upon infection. The present study evaluated two kinds of vaccine adjuvants for use with recombinant S protein: gold nanoparticles (AuNPs) and Toll like receptor (TLR) agonists. The AuNP‐adjuvanted protein induced a strong IgG response but failed to improve vaccine efficacy or to reduce eosinophilic infiltration because of highly allergic inflammatory responses. On the other hand, the TLR agonist‐adjuvanted vaccine induced highly protective antibodies without eosinophilic infiltrations. The findings of this study will support the development of vaccines against severe pneumonia‐associated coronaviruses.

Academic Significance and Societal Importance of the Research Achievements

SARSはアジアを中心に2003年に大流行した重症呼吸器感染症で、現在は終息しているが再興の可能性がある。そしてMERSは2012年に中東で発見された新規のヒトコロナウイルスであるMERS-CoVが原因で重症肺炎を起こす。現在も患者数は増加し、2015年の春には隣国の韓国で、病院内のアウトブレイクが起こり、一大ニュースとなった。これらの重症呼吸器感染症に対して、ハイリスク者および医療従事者へのワクチン接種が流行防止措置として上げられるが、候補となるワクチンは未だにない。本研究で得られた結果は、重症肺炎を起こすヒトコロナウイルスに対する新規ワクチンの開発において、それをサポートするものである。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Gold nanoparticle-adjuvanted S protein induces a strong antigen-specific IgG response against severe acute respiratory syndrome-related coronavirus infection, but fails to induce protective antibodies and limit eosinophilic infiltration in lungs.2019

    • Author(s)
      Hanako Sekimukai, Naoko Iwata-Yoshikawa, Shuetsu Fukushi, Hideki Tani, Michiyo Kataoka, Tadaki Suzuki, Hideki Hasegawa, Kenichi Niikura, Katsuhiko Arai, Noriyo Nagata.
    • Journal Title

      Microbiology and Immunology

      Volume: 64 Issue: 1 Pages: 33-51

    • DOI

      10.1111/1348-0421.12754

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Acute respiratory infection in human dipeptidyl peptidase 4-transgenic mice infected with Middle East respiratory syndrome coronavirus2019

    • Author(s)
      Naoko Iwata-Yoshikawa, Tadashi Okamura, Yukiko Shimizu, Osamu Kotani, Hironori Sato, Hanako Sekimukai, Shuetsu Fukushi, Tadaki Suzuki, Yuko Sato, Makoto Takeda, Masato Tashiro, Hideki Hasegawa, Noriyo Nagata.
    • Journal Title

      J Virol.

      Volume: 93 Issue: 6

    • DOI

      10.1128/jvi.01818-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] TMPRSS2 contributes to virus spread and immunopathology in the airways of murine models after coronavirus infection.2019

    • Author(s)
      Iwata-Yoshikawa N, Okamura T, Shimizu Y, Hasegawa H, Takeda M, Nagata N
    • Journal Title

      J Virol

      Volume: 93 Issue: 6

    • DOI

      10.1128/jvi.01815-18

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 重症肺炎を引き起こすヒトコロナウイルスの新規ワクチン開発.2019

    • Author(s)
      関向 華子、岩田 奈織子、福士 秀悦、福間 藍子、谷  英樹、長谷川 秀樹、永田 典代.
    • Organizer
      第67回日本ウイルス学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The severity of Middle East respiratory syndrome in human dipeptidyl peptidase 4 (DPP4)-bearing transgenic mice.2018

    • Author(s)
      Naoko Iwata-Yoshikawa, Tadashi Okamura, Makoto Takeda, Hideki Hasegawa, and Noriyo Nagata.
    • Organizer
      Keystone symposia n molecular and cellular Biology, Framing the response to emerging virus infections (S2)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] TMPRSS2 plays a role in replication and pathogenesis of SARS-CoV and MERS-CoV in mice.2018

    • Author(s)
      Naoko Iwata-Yoshikawa, Tadashi Okamura, Shutoku Matsuyama, Makoto Takeda, Hideki Hasegawa, and Noriyo Nagata.
    • Organizer
      Keystone symposia n molecular and cellular Biology, Framing the response to emerging virus infections (S2)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expression and characterization of spike proteins of human coronavirus associated with severe pneumonia.2017

    • Author(s)
      Hanako Sekimukai, Naoko Iwata-Yoshikawa, Aiko Fukuma, Shuetsu Fukushi, Hideki Tani, Makoto Takeda, Katsuhiko Arai, Hideki Hasegawa, Noriyo Nagata.
    • Organizer
      XIVth International Nidovirus Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of a vaccine against human coronavirus using gold nanoparticles.2017

    • Author(s)
      Hanako Sekimukai, Naoko Iwata-Yoshikawa, Aiko Fukuma, Shuetsu Fukushi, Hideki Tani, Michiyo Kataoka, Katsuhiko Arai, Kenichi Niikura, Hideki Hasegawa, Noriyo Nagata
    • Organizer
      第40 回日本分子生物学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 重症肺炎を引き起こすヒトコロナウイルスのスパイクタンパク質の作製とその有用性について2016

    • Author(s)
      関向華子、岩田(吉河)奈織子、福間藍子、福士秀悦、谷 英樹、長谷川秀樹、永田典代
    • Organizer
      日本ウイルス学会
    • Place of Presentation
      北海道、札幌、札幌コンベンションセンター
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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