Analysis of pathogenic mechanism of fulminant pneumococcal infection

Research Project

Project/Area Number	16K09953
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Infectious disease medicine
Research Institution	National Institute of Infectious Diseases
Principal Investigator	CHANG BIN 国立感染症研究所, 細菌第一部, 主任研究官 (50370961)
Project Period (FY)	2016-04-01 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	感染症防御学 / 肺炎球菌 / 劇症型肺炎球菌感染症 / 病原性 / 劇症型 / 劇症型感染症 / ゲノム解析 / 全ゲノム解析 / 病原因子 / 次世代シーケンス / 宿主―細菌相互作用
Outline of Final Research Achievements	In order to elucidate the mechanism of Streptococcus pneumoniae that causes fulminant infections, we carried out various analysis from aspects such as genome analysis, in vitro, and in vivo experiments. However, no clear difference could be found between pneumococcal strains isolated from fulminant and non-fulminant infections. It has been suggested that host factors play an important role in the development of fulminant pneumococcal infection. On the other hand, it cannot be denied that the experimental methods widely used for the pathogenicity of bacteria are not suitable for S. pneumoniae. Since the pathogenicity of 22F pneumococci could be evaluated in C57BL/6 mice using nasal administration, we plan to proceed with the analysis focusing on 22F pneumococci in the future.
Academic Significance and Societal Importance of the Research Achievements	(1)肺炎球菌の発症機構の解析により、宿主側のファクターが重要な役割を果たす可能性を見出した。また、劇症型肺炎球菌感染症から多く分離される23F、22F、10A、6Bなど血清型は肺炎球菌ワクチンに含まれているため、予防接種による感染症予防の重要性を再認識でき、今後発信する科学的根拠になる。 (2)本研究はゲノム解析やin vitroとin vivoなどさまざまな実験方法によって肺炎球菌を解析してきた、今後の研究の基礎となる。