Selective antibacterial activity of peptide nucleic acid against Streptococcus pneumoniae

Research Project

Project/Area Number	16K10016
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatrics
Research Institution	Niigata University
Principal Investigator	Otsuka Taketo 新潟大学, 医歯学系, 助教 (20772007)
Project Period (FY)	2016-04-01 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	肺炎球菌 / アンチセンス / ペプチド核酸 / 必須遺伝子 / 薬剤耐性 / PNA / バイオフィルム
Outline of Final Research Achievements	To assess antibacterial activity against Streptococcus pneumoniae, we designed PNA-peptides. A PNA-peptide （RFR-acpP-PNA2S）that targets acpP, has a 5′ membrane-penetrating peptide and has a linker shows excellent activity against planktonic Streptococcus pneumoniae（MIC 5μmol/L）.
Academic Significance and Societal Importance of the Research Achievements	従来の抗菌薬はその濫用により耐性化が問題となっている。本研究で開発した新しい抗菌薬であるPNAは、肺炎球菌だけが持つ遺伝子配列をターゲットにすることで同菌を選択的に除菌することができる。