Mechanistic studies to analyze the pathophysiology of ISSNS in children

• Project/Area Number: 16K10073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Jichi Medical University
• Principal Investigator: Kanai Takahiro 自治医科大学, 医学部, 准教授 (00398504)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 小児特発性ステロイド感受性ネフローゼ症候群 / 小児 / ステロイド感受性ネフローゼ症候群 / 脂質 / T細胞 / トランスレーショナルリサーチ / B細胞 / 転写因子

Outline of Final Research Achievements

This study was aimed to investigate the role of STAT5B in pediatric idiopathic steroid-sensitive nephrotic syndrome and to reveal the pathophysiology, then to contribute to develop new medicines for this disease.
ChIP-seq technique was applied for this study, using the patients’ paired-peripheral blood mononuclear cells from nephrotic-phase before initiation of steroids and from remission phase without any medication.
Several peaks of binding-sites were detected. The peaks were statistically different between nephrotic-phase and remission-phase. Some of them were the binding sites for vascular permeability, cellular or humoral immunity or lipid metabolism.

Academic Significance and Societal Importance of the Research Achievements

本研究により、小児特発性ステロイド感受性ネフローゼ症候群の病態の一部を解明する手掛かりを得ることができた。本研究を進めることで、原因のさらなる解明を行うことができると思われる。