| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
In this study, I analyzed the molecular mechanism of a novel cholesterol trafficking to communicate across the organelle membrane between endosomes and mitochondria in the placental cells. As a result, I used immuno-electron tomography to analyze 3D structures of approximately 20-nm distance of the late endosome-mitochondria contact sites, and identified filamentous structures, tethers, that connected between the late endosomes and mitochondria. The tethers connected with the late endosomes and mitochondria were specifically gone in the MLN64 knockdown cells. My results suggest that ultra-structural features of MLN64-dependent membrane contacts contribute the production of progesterone in the placental cells.
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