Protection of neonatal hypoxic-ischemic brain injury by transplantation of rat umbilical cord blood cells
Project/Area Number |
16K10119
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Institute for Developmental Research Aichi Developmental Disability Center |
Principal Investigator |
NAKANISHI KEIKO 愛知県医療療育総合センター発達障害研究所, 障害モデル研究部, 非常勤研究員 (50280813)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | 新生児低酸素性虚血性脳症 / 臍帯血幹細胞 / 移植再生医療 / 周生期脳障害 / 神経科学 |
Outline of Final Research Achievements |
Mononuclear cells isolated from GFP-transgenic rat umbilical cord blood were expanded (stem cell enriched-umbilical cord blood cells; SCE-UCBCs) and administered intraperitoneally to neonatal hypoxic-ischemic encephalopathy (HIE) model of rats. The GFP-positive cells were hardly detectable in the brain. The GFP-positive cells in the brain were negative for neuronal markers. These results indicate that UCBCs could ameliorate HI injury, possibly through an endogenous response not by supplying differentiated neurons derived from the injected stem cells.
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Academic Significance and Societal Importance of the Research Achievements |
新生児低酸素性虚血性脳症(HIE)は現在もなお、脳性まひの主な原因の一つである。臨床的に低体温療法が行われているが、重症例に対する効果は急性期に限局しており、神経後遺症の克服のために幹細胞療法など新規治療法の開発が期待されている。臍帯血にも幹細胞が含まれることから、日本でも自家臍帯血移植の臨床研究が始まっているが、幹細胞移植による脳障害軽減機序は未だ明らかではない。本研究で、この機序が明らかになれば、臨床研究のさらなる進展や効率的な移植方法の開発につながることが期待できる。
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Administration of Bone Marrow-Derived Mononuclear Cells Contributed to the Reduction of Hypoxic-Ischemic Brain Injury in Neonatal Rats2018
Author(s)
Sato Y, Kondo T, Ueda K, Hattori T, Mikrogeorgiou A, Sugiyama Y, Suzuki T, Yamamoto M, Hirata H, Hirakawa A, Nakanishi K, Tsuji M, Hayakawa M.
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Journal Title
Front. Neurol
Volume: 9
Pages: 987-987
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy.2017
Author(s)
Mikrogeorgiou A, Kondo T, Hattori T, Sugiyama Y, Nakanishi K, Tsuji M, Kazama T, Kano K, Matsumoto T, Hayakawa M, Sato Y
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Journal Title
Developmental Neuroscience
Volume: 印刷中
Issue: 1-4
Pages: 273-286
DOI
Related Report
Peer Reviewed
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[Presentation] Distinct role of SAD-A and SAD-B in radial neuronal migration in the developing brain2019
Author(s)
Keiko Nakanishi, Hiroyuki Niida, Hidenori Tabata, Yoshikazu Johmura, Takashi Ueda, Kenichiro Yamada, Koh-ichi Nagata, Nobuaki Wakamatsu, Masashi Kishi, Shinya Ugawa, Shoichi Shimada, Yujiro Higashi, Makoto Nakanishi
Organizer
第42回日本神経科学大会/第62回日本神経化学会大会
Related Report
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[Presentation] Role of SAD-A kinase in radial neuronal migration during development of cerebral cortex.2019
Author(s)
Nakanishi K, Niida H, Tabata H, Johmura Y, Yamada K, Nagata K-I, Wakamatsu N, Kishi M, Higashi Y, Nakanishi M
Organizer
9th Federation of the Asian and Oceanian Physiological Societies(FAOPS) Congress,
Related Report
Int'l Joint Research
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[Presentation] Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy2017
Author(s)
Alkisti Mikrogeorgiou, Yoshiaki Sato, Taiki Kondo, Tetsuo Hattori, Yuichiro Sugiyama, Keiko Nakanishi, Masahiro Tsuji, Tomohiko Kazama, Koichiro Kano, Taro Matsumoto, Masahiro Hayakawa
Organizer
Pediatric Academic Societies Annual Meeting 2017 (PAS 2017)
Related Report
Int'l Joint Research
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[Presentation] Dedifferentiated fat cells, as a novel source for cell therapy to target neonatal hypoxic ischemic encephalopathy,2017
Author(s)
Yoshiaki Sato, Alkisti Mikrogeorgiou, Taiki Kondo, Tetsuo Hattori, Yuichiro Sugiyama, Keiko Nakanishi, Masahiro Tsuji, Tomohiko Kazama, Koichiro Kano, Taro Matsumoto, Masahiro Hayakawa
Organizer
International Society for Stem Cell Research (ISSCR) Annual meeting2017
Related Report
Int'l Joint Research
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[Presentation] Dedifferentiated fat cells as a treatment for perinatal hypoxic ischemic brain injury.2016
Author(s)
Mikrogeorgiou A, Sato Y, Kondo T, Sugiyama Y, Nakanishi K, Tsuji M, Kazama T, Matsumoto T, Kano K, Hayakawa M.
Organizer
10th Hershey conference on Developmental brain Injury
Place of Presentation
Chateau de Romainville, France
Year and Date
2016-06-08
Related Report
Int'l Joint Research