| Project/Area Number |
16K10128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Ehime University |
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Principal Investigator |
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| Research Collaborator |
Tsuda Teruko
Tan Eriko
Kameda Kenji
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 掌蹠膿疱症 / 抗菌ペプチド / hCAP-18 / TLN-58 / エクリン汗腺 / cathelicidin / 皮膚病態学 |
|---|
| Outline of Final Research Achievements |
The abnormal fragment of hCAP-18 in PPP vesicle was searched with several experiments, and TLN-58 was confirmed. The responsible proteinase was searched with MASCOT database, and neutrophilic elastase was found in the database. As a proteinase inhibitor for it, alpha one antitrypsin was selected and tested if it could suppress the protein processing of hCAP-18 to TLN-58, and we could confirm the prevention of processing. TLN-58 synthetic peptide induces the inflammatory response in primary keratinocyte culture and LSE, but not in sweat gland cells (NCL-SG3), especially for IL-8, comparing with LL-37. In the mouse model, the peptide could induce inflammatory changes in skin tissue, too. However, the proteinase inhibitor induced inflammatory response as an irritant, so that we failed to establish it as a new treatment for PPP.
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| Academic Significance and Societal Importance of the Research Achievements |
掌蹠膿疱症の病態に関わる因子がまた新たに一つ同定された。水疱内容における異常な抗菌ペプチドの発現様式が本疾患の病態に関わることが明らかとなり、将来の治療オプションを考える上で、重要な発見となった。
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