| Project/Area Number |
16K10169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Asai Jun 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50438222)
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| Co-Investigator(Kenkyū-buntansha) |
田畑 泰彦 京都大学, ウイルス・再生医科学研究所, 教授 (50211371)
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| Research Collaborator |
Kanemaru Mai
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ナノ粒子 / 腫瘍特異的リンパ球 / 皮膚悪性腫瘍 / 悪性黒色腫 / 癌 / ナノバイオ |
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| Outline of Final Research Achievements |
First, we prepared docetaxel or coumarin-loaded PLGA nanoparticles(DOC-NP, FL-NP). nanoparticles were prepared by an emulsion solvent evaporation method.In vitro cellular uptake of FL-NP by lymphocytes was confirmed with confocal laser microscope. The anti-tumor effects of DOC-NP on angiosarcoma cells were evaluated by proliferation assay and FACS analysis, however, DOC-NP did not show significant anti-tumor effects. It would be because low uptake of docetaxel into lymphocytes. Further device would be necessary to improve drug uptake to lymphocytes.
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| Academic Significance and Societal Importance of the Research Achievements |
今までにリンパ球をドラッグデリバリーシステムに応用するという試みはされておらず、本研究は、リンパ球をドラッグキャリアとして利用する初の試みであり、有意な抗腫瘍効果は認められなかったものの、リンパ球への薬剤取り込みが確認出来たことより、今後の新たなリンパ球を用いたドラッグデリバリーシステムの可能性を見出した研究であるといえる。
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