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Research of Natural History and Hepatocellular Carcinoma in NASH Model Mice by MRI and Optical Imaging

Research Project

Project/Area Number 16K10308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Radiation science
Research InstitutionInternational University of Health and Welfare (2017-2019)
The University of Tokyo (2016)

Principal Investigator

Kiryu Shigeru  国際医療福祉大学, 医学部, 教授 (20313124)

Co-Investigator(Kenkyū-buntansha) 大田 泰徳  (財)冲中記念成人病研究所, その他部局等, 研究員 (60376431)
赤井 宏行  東京大学, 医科学研究所, 講師 (80770437)
潮見 隆之  国際医療福祉大学, 医学部, 教授 (80348797)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsNASH / 肝細胞癌 / MRI / 光イメージング / 肝臓 / 核磁気共鳴画像(MRI)
Outline of Final Research Achievements

This study clarified the natural history of NASH model mice in Gd-EOB-DTPA-enhanced MRI and fluorescence imaging using ICG as a platform for NASH research and hepatocellular carcinoma research. Hepatocellular carcinoma was observed on imaging by 10 weeks and was pathologically proven to be hepatocellular carcinoma. Based on these findings, we examined hepatocellular carcinoma in NASH model mice by MRI and fluorescence imaging, and compared the results with those obtained by immunostaining.

Academic Significance and Societal Importance of the Research Achievements

本研究ではマウスを用いて安定したNASHモデルを作成、さらにMRIおよび蛍光光イメージングにより観察が行えることを示した。画像による研究はマウスをsacrificeすることなく、同一個体で繰り返し観察を行うことが可能であり、今後のNASHの研究に貢献すると考えられる。また、NASHモデルマウスに発生する肝細胞癌はGd-EOB-DTPAやICGを取り込む頻度がヒトよりも高いことが判明した。今後詳細なトランスポーターの研究を進行させることにより、ヒトにおけるGd-EOB-DTPAやICG、さらに胆汁酸の肝細胞における取り込みのメカニズムの解明につながる可能性が期待される。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 2 results)

  • [Journal Article] Influence of Indocyanine Green on Hepatic Gd-EOB-DTPA Uptake: A Proof-of-Concept Study in Mice.2017

    • Author(s)
      Akai H, Yasaka K, Nojima M, Inoue Y, Ohtomo K, Kiryu S.
    • Journal Title

      Invest Radiol.

      Volume: 印刷中 Issue: 7 Pages: 441-445

    • DOI

      10.1097/rli.0000000000000359

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The natural history of streptozotocin-stimulated non-alcoholic steatohepatitis mice followed by Gd-EOB-DTPA-enhanced MRI: Comparison with simple steatosis mice.2017

    • Author(s)
      Akai H, Kiryu S, Ohta Y, Yasaka K, Nakano Y, Inoue Y, Ohtomo K.
    • Journal Title

      Magn Reson Imaging.

      Volume: 38 Pages: 123-128

    • DOI

      10.1016/j.mri.2016.12.027

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant

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Published: 2016-04-21   Modified: 2021-02-19  

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