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Biological response to hypoxic condition after radiation with X rays or carbon ions

Research Project

Project/Area Number 16K10409
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Radiation science
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

Liu Cuihua  国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 主任研究員(任非) (00512427)

Co-Investigator(Kenkyū-buntansha) 平山 亮一  国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 重粒子線治療研究部, 主任研究員(定常) (90435701)
Research Collaborator Fujimori Akira  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords低酸素応答 / 低酸素培養 / 放射線 / 染色体異常 / 相同組換え / 低酸素 / 炭素線 / 低酸素環境 / 細胞周期 / 酸素効果 / 高LET / 相同組換え頻度 / 重粒子線 / 低酸素応答機構 / 応答機構 / 染色体修復忠実度 / NrF2
Outline of Final Research Achievements

Mouse kidney primary culture cell and human glioblastom cell lines were used in this study. Cell lethality, chromosome aberration, cell cycle distribution and homologous recombination were estimated when cells cultured in normal oxygen (21% O2) or hypoxic conditions (1%, 3% O2) after radiations. Compared to a normal oxygen condition, as a result of culturing in different oxygen conditions, the survival fractions of primary cultured mouse kidney cells are increased and chromosomal abnormal frequencies (abnormal cells, exchange, fragments) are decreased when cells cultured in hypoxic conditions after X-rays or high LET carbon ion radiation; those phenomenon was thought to be related to homologous recombination but there was lower frequencies of homologous recombination when cells cultured in a hypoxic condition. Therefore, may be there no direct correlation between increased survival fraction and homologous recombination frequency when cells cultured in hypoxic conditions.

Academic Significance and Societal Importance of the Research Achievements

X線特に高LET放射線に対する照射後の低酸素ストレス応答についての研究報告はほとんどされていなかったため、本課題では生体内低酸素環境を模擬した実験系において、細胞の放射線応答を明らかにした研究である。長時間による低酸素環境での放射線応答、染色体損傷修復および相同組換え修復効率についての知見を初めて取得することができた。生体の放射線応答を考える上、生体内酸素濃度の重要性を明らかにすることができ、今まで考慮されなかった“生体内酸素濃度”を放射線がん治療や宇宙科学研究に組み込むことで、放射線の効果を最大限に活かした放射線健康科学に本研究の新知見が活かされると考える。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Funded Workshop (1 results)

  • [Journal Article] Emerging Role of Secondary Bystander Effects Induced by Fractionated Protons Microbeam2019

    • Author(s)
      Narongchai Autsavapromporn , Cuihua Liu , Alisa Kobayashi , Tengku Ahbrizal Farizal Tengku Ahmad , Masakazu Oikawa , Nahathai Dukaew , Jun Wang , Ariyaphong Wongnoppavich , Teruaki Konishi
    • Journal Title

      Radiation Research

      Volume: 191(2) Issue: 2 Pages: 211-216

    • DOI

      10.1667/rr15155.1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reduction of Delayed Homologous Recombination by Induction of Radioadaptive Response in RaDR-GFP Mice (Yonezawa Effect): An Old Player with A New Role2019

    • Author(s)
      Cuihua Liu , Hirokazu Hirakawa , Kaoru Tanaka , Fazliana Mohd saaya , Mitsuru Nenoi , Akira Fujimori , Bing Wang
    • Journal Title

      Dose-Response

      Volume: 17(1) Issue: 1

    • DOI

      10.1177/1559325819833840

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased Hematopoietic Stem Cells/Hematopoietic Progenitor Cells Measured as Endogenous Spleen Colonies in Radiation-Induced Adaptive Response in Mice (Yonezawa Effect)2018

    • Author(s)
      Wang Bing、Tanaka Kaoru、Ninomiya Yasuharu、Maruyama Kouichi、Var?s Guillaume、Katsube Takanori、Murakami Masahiro、Liu Cuihua、Fujimori Akira、Fujita Kazuko、Liu Qiang、Eguchi-Kasai Kiyomi、Nenoi Mitsuru
    • Journal Title

      Dose-Response

      Volume: 16 Issue: 3 Pages: 1-10

    • DOI

      10.1177/1559325818790152

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mouse intestinal Lgr5+ stem cells are more sensitive to heavy ion irradiation than Bmi1+ stem cells2018

    • Author(s)
      Anqing Wu , Wentao Hu , Jian Zhang , Guo Ziyang , Cuihua Liu , Takanori Katsube , Kaoru Tanaka , Jing Nie , Bing Wang , Guangming Zhou
    • Journal Title

      Acta Biochimica et Biophysica Sinica

      Volume: 51(3) Issue: 3 Pages: 338-340

    • DOI

      10.1093/abbs/gmy158

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Impact of Co-Culturing with Fractionated Carbon-Ion-Irradiated Cancer Cells on Bystander Normal Cells and Their Progeny2017

    • Author(s)
      N Autsavapromporn, C Liu, T Konishi.
    • Journal Title

      Radiation Research

      Volume: 188 Issue: 3 Pages: 335-341

    • DOI

      10.1667/rr14773.1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RAC2-P38 MAPK-dependent NADPH oxidase activity is associated with the resistance of quiescent cells to ionizing radiation2017

    • Author(s)
      Pei H, Zhang J, Nie J, Ding N, Hu W, Hua J, Hirayama R, Furusawa Y, Liu C, Li B, Hei TK, Zhou G
    • Journal Title

      Cell Cycle

      Volume: 16 Issue: 1 Pages: 113-122

    • DOI

      10.1080/15384101.2016.1259039

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nontoxic concentration of DNA-PK inhibitor NU7441 radio-sensitizes lung tumor cells with little effect on DSB repair.2016

    • Author(s)
      Shigeaki Sunada, Hideki Kanai, Younghyun Lee, Takeshi Yasuda, Hirokazu Hirakawa, Cuihua Liu, Akira Fujimori, Mitsuru Uesaka, Ryuichi Okayasu
    • Journal Title

      Cancer Science

      Volume: 107 Issue: 9 Pages: 1250-1255

    • DOI

      10.1111/cas.12998

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] X線や炭素線照射後の生体応答に対する低酸素環境の役割に関する研究2018

    • Author(s)
      劉 翠華 , 平川 博一 , 平山 亮一 , 藤森 亮
    • Organizer
      日本放射線影響学会第61回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Mechanism of cell lethal effect in mesothelioma by high LET carbon-ion beams2017

    • Author(s)
      C.Liu , N. Ausavaproporn, A. Fujimori
    • Organizer
      The 5th International Symposium on space radiation and particle radiotherapy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] X線照射後の生体応答に対する低酸素環境の役割に関する研究2017

    • Author(s)
      劉翠華, 平川博一, 藤森亮, 平山亮一
    • Organizer
      日本放射線影響学会第60回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 放射線誘発マウス胸腺腫およびゲノム不安定性の検討2016

    • Author(s)
      劉 翠華、平川博一、王 冰、中島徹夫、勝部孝則、二宮康晴、田中薫、根井充、藤森 亮
    • Organizer
      放射線影響学会第59回大会
    • Place of Presentation
      広島JMSアステールプラザ
    • Year and Date
      2016-10-25
    • Related Report
      2016 Research-status Report
  • [Funded Workshop] The 5th International Symposium on space radiation and particle therapy2017

    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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