| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
Rat hepatocytes were isolated using a modified two-step collagenase perfusion technique and then purified by Percoll density gradient centrifugation. Hepatocytes (1.0 × 107) with a viability exceeding 90% were directly injected into the portal vein (n=8), spleen pulp (n=8), liver parenchyma, and so on, on the analbuminemic rats using a gastight syringe for 60 seconds. Blood samples were taken pre-transplantation and at weeks 2, 4, 6, 8, 10, 12, and 16 after transplantation. The serum albumin levels were quantified using a rat albumin enzyme-linked immunosorbent assay kit.
As a result, the serum albumin levels of the intrasplenic group (pre-transplantation: 7.55±0.65 μg/ml, Week 2: 55.00±21.02 μg/ml, Week 4: 79.88±32.62 μg/ml, Week 6: 105.96±54.46 μg/ml, Week 8: 114.73±58.34 μg/ml, Week 10: 116.11±56.33 μg/ml, Week 12: 122.76±60.85 μg/ml, and Week 16: 133.96±66.03 μg/ml, respectively) were significantly lower than those of the intraportal group
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