Hybrid transplantation of marginal donor grafts and hepatocytes using biological affinity cell sheet
Project/Area Number |
16K10417
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Tohoku University |
Principal Investigator |
Miyazawa Koji 東北大学, 医学系研究科, 非常勤講師 (80757237)
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Co-Investigator(Kenkyū-buntansha) |
宮城 重人 東北大学, 大学病院, 准教授 (00420042)
後藤 昌史 東北大学, 医学系研究科, 教授 (50400453)
原 康之 東北大学, 医学系研究科, 非常勤講師 (50636008)
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Research Collaborator |
Hara Yasuyuki 東北大学, 大学病院, 非常勤講師 (50636008)
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
|
Keywords | 線維芽細胞シート被覆肝細胞 / 門脈内移植 / 肝臓実質内移植 / 脾臓実質内移植 / 原始免疫反応 / 自己細胞シート / 肝細胞移植 / 親生体素材シート / 自己線維芽細胞シート / 被覆肝細胞 / 細胞移植 / 門脈圧亢進 / 類洞微小循環 / 移植・再生医療 |
Outline of Final Research Achievements |
Rat hepatocytes were isolated using a modified two-step collagenase perfusion technique and then purified by Percoll density gradient centrifugation. Hepatocytes (1.0 × 107) with a viability exceeding 90% were directly injected into the portal vein (n=8), spleen pulp (n=8), liver parenchyma, and so on, on the analbuminemic rats using a gastight syringe for 60 seconds. Blood samples were taken pre-transplantation and at weeks 2, 4, 6, 8, 10, 12, and 16 after transplantation. The serum albumin levels were quantified using a rat albumin enzyme-linked immunosorbent assay kit. As a result, the serum albumin levels of the intrasplenic group (pre-transplantation: 7.55±0.65 μg/ml, Week 2: 55.00±21.02 μg/ml, Week 4: 79.88±32.62 μg/ml, Week 6: 105.96±54.46 μg/ml, Week 8: 114.73±58.34 μg/ml, Week 10: 116.11±56.33 μg/ml, Week 12: 122.76±60.85 μg/ml, and Week 16: 133.96±66.03 μg/ml, respectively) were significantly lower than those of the intraportal group
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Academic Significance and Societal Importance of the Research Achievements |
細胞の大きさで生着率が変わるのではなく個々の細胞の性質で生着率が変わることが分かった点、最も生着効率が高いのは結果的には門脈内肝細胞移植であることが分かった点、肝細胞は線維芽細胞シート被覆により原始免疫反応を回避する効果があり生着率が上がることが分かった点で、学術的意義が高いと考えられる
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Correlation Between Splenectomy and Portal Vein Complications in Living Donor Liver Transplantation.2018
Author(s)
Miyagi S, Nakanishi C, Hara Y, Nakanishi W, Tokodai K, Shimizu K, Uematsu S, Kumata H, Kakizaki Y, Goto M, Kamei T, Unno M, Satomi S.
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Journal Title
Transplant Proc. 2018 Nov;50(9):2611-2613.
Volume: 50(9)
Issue: 9
Pages: 2611-2613
DOI
Related Report
Peer Reviewed
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[Journal Article] Effect of Middle Hepatic Vein Tributaries Preserving Technique Until Just Before Graft Retrieval on Donors' Surgical Outcomes in Living Donor Liver Transplantation.2018
Author(s)
Miyazawa K, Miyagi S, Nakanishi C, Hara Y, Tokodai K, Nakanishi W, Uematsu S, Shimizu K, Goto M, Kamei T, Unno M.
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Journal Title
Transplant Proc. 2018 Jun;50(5):1538-1543.
Volume: 50(5)
Issue: 5
Pages: 1538-1543
DOI
Related Report
Peer Reviewed
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[Journal Article] The effects of short-term subnormothermic perfusion after cold preservation on liver grafts from donors after cardiac death: An ex vivo rat model2018
Author(s)
Kakizaki Y, Miyagi S, Shimizu K, Miyazawa K, Nakanishi W, Tokodai K, Hara Y, Nakanishi C, Unno M, Kamei T, Goto M, Satomi S
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Journal Title
Transplantation
Volume: 102
Issue: 4
Pages: e147-e154
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Arterial and biliary complications after living donor liver transplantation: a single-center retrospective study and literature review.2018
Author(s)
Miyagi S, Kakizaki Y, Shimizu K, Miyazawa K, Nakanishi W, Hara Y, Tokodai K, Nakanishi C, Kamei T, Ohuchi N, Satomi S.
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Journal Title
Surg Today
Volume: 48(2)
Issue: 2
Pages: 131-139
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Optimization of Short-Term Hepatocyte Preservation Before Transplantation.2017
Author(s)
Fukuoka K, Inagaki A, Nakamura Y, Matsumura M, Yoshida S, Imura T, Igarashi Y, Miyagi S, Ohashi K, Enosawa S, Kamei T, Unno M, Ohuchi N, Satomi S, Goto M.
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Journal Title
Transplant Direct.
Volume: 19;3(7)
Issue: 7
Pages: e176-e176
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Successful Simultaneous Pancreas-Kidney Transplantation Using the Injured Pancreas Graft2017
Author(s)
Miyagi S, Shimizu K, Miyazawa K, Nakanishi W, Hara Y, Tokodai K, Nakanishi C, Satomi S, Goto M, Unno M, Kamei T.
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Journal Title
Transplant Proc.
Volume: 49(10)
Issue: 10
Pages: 2315-2317
DOI
Related Report
Peer Reviewed
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[Journal Article] The reconstruction of hepatic arteries using extra-anatomical free autografts in living donor liver transplantation2016
Author(s)
Miyagi S, Shimizu K,Miyazawa K, Kakizaki Y, Fujio A, Hara Y, Nakanishi C, Goto H,Kamei T,Kawagishi N, Ohuchi N, and Satomi S.
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Journal Title
J Transplant Technol Res
Volume: 7:2
Issue: 02
Pages: 157-157
DOI
Related Report
Peer Reviewed
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[Presentation] The optimization of the hepatocyte surface modification procedures in terms of heparin and apyrase for improving hepatocyte engraftment2018
Author(s)
Hiroyuki Ogasawara1, Yuji Teramura2,3, Takehiro Imura4, Akiko Inagaki4, Yoshikatsu Saito1, Muneyuki Matsumura1, Kengo Fukuoka1, Ibrahim Fathi4, Shigehito Miyagi1, Kristina Nilsson3, Kazuo Ohashi5, Michiaki Unno1, Takashi Kamei1, Susumu Satomi1, Bo Nilsson3, Elisabet Gustafson6, Masafumi Goto1,2
Organizer
27th International Congress of The Transplantation Society
Related Report
Int'l Joint Research
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[Presentation] A comparison of the transplant efficiency between intraportal and intrasplenic procedures in hepatocyte transplantation2018
Author(s)
Hiroyuki Ogasawara1, Akiko Inagaki2, Takehiro Imura2, Yoshikatsu Saito1, Muneyuki Matsumura1, Kengo Fukuoka1, Ibrahim Fathi2, Shigehito Miyagi1, Kazuo Ohashi3, Michiaki Unno1, Takashi Kamei1, Susumu Satomi1 and Masafumi Goto1,2
Organizer
27th International Congress of The Transplantation Society
Related Report
Int'l Joint Research
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[Presentation] The risk factors for portal vein thrombosis after living donor liver transplantation in adults2018
Author(s)
Shigehito Miyagi, Chikashi Nakanishi, Yasuyuki Hara, Wataru Nakanishi, Kazuaki Tokodai, Kenji Shimizu, Satomi Uematsu, Hiroyuki Kumata, Yuta Kakizaki, Masafumi Goto, Susumu Satomi, Michiaki Unno and Takashi Kamei
Organizer
27th International Congress of The Transplantation Society
Related Report
Int'l Joint Research
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[Presentation] The effects of resveratrol on liver transplantation from non-heart-beating donors in pigs.2016
Author(s)
Kenji Shimizu1, Shigehito Miyagi1, Koji Miyazawa1, Kai Maida1, Toshiaki Kashiwadate1,Yasuyuki Hara1, Masafumi Goto1,2, Naoki Kawagishi1, Noriaki Ohuchi1 and Susumu Satomi1
Organizer
国際移植学会
Place of Presentation
HongKong, China
Year and Date
2016-08-19
Related Report
Int'l Joint Research