Development of recurrence risk prediction model 95-gene classifier and its application to formalin-fixed paraffin-embedded tissues.
| Project/Area Number |
16K10456
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Naoi Yasuto 大阪大学, 医学系研究科, 助教 (30646211)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 乳癌 / マイクロアレイ / 再発予測 / 多遺伝子アッセイ / FFPE / 網羅的遺伝子発現解析 / RNA |
|---|
| Outline of Final Research Achievements |
We previously developed a 95-gene classifier (95GC) to classify ER-positive, HER2-negative, and node-negative breast cancers as high and low-risk groups. Although the 95GC was originally developed using fresh-frozen (FF) tissues, we aimed to apply this to formalin-fixed paraffin-embedded (FFPE) tissues.
As the result, 95GC Recurrence Score was well correlated (R = 0.92) with FF and FFPE tissues, and the concordance rate (94.6%) of high- and low-risk groups was also considerably high. Our 95GC was applicable to FFPE tissues with a high concordance rate with FF tissues.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究では、Curebest 95GCをホルマリン固定パラフィン包埋組織検体（以下、FFPE検体）にも応用可能なように改良した。本研究により95GCはFFPEに適応拡大することに成功し、2019年現在多数の病院施設からのFFPE検体を受け付けている。この事で、より多数の乳癌患者を対象に再発予後予測を施行し、癌個別化医療を実現する事ができた。またFFPE検体を多く有する米国4施設において、95GC性能検証研究を進め、良好な成績を得る事ができた。
|
Report
(4 results)
Research Products
(15 results)
