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Comprehensive analysis for the association between microbiome in the gastric mucosa and malignant potential of gastric cancer

Research Project

Project/Area Number 16K10504
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionKumamoto University

Principal Investigator

YOSHIDA Naoya  熊本大学, 医学部附属病院, 特任准教授 (60467983)

Co-Investigator(Kenkyū-buntansha) 今村 裕  公益財団法人がん研究会, 有明病院 消化器外科, 副医長 (70583045)
井田 智  公益財団法人がん研究会, 有明病院 消化器外科, 副医長 (80583038)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsFusobacterium / F. nucleatum / microbiome / 胃癌 / 食道胃接合部癌 / 予後因子 / マイクロバイオーム / 食道胃接合部腺癌 / 予後 / PCR
Outline of Final Research Achievements

In this study, we developed a detecting method of F. nucleatum, targeting nusG, using real-time PCR. We examined the clinicopathological, molecular characteristics, and prognostic effect of the tumor with F. nucleatum (+) tumors, compared to those without. In a total of 115 cases with gastroesophageal adenocarcinoma, F. nucleatum was detected in 40 cases (34.8%). We analyzed factors like gender, age, tumor location, H pylori infection status by gastroscopy, disease stage, PD-L1 expression on tumor cells, intra-/peri-tumoral CD8(+) lymphocyte infiltration, intra-/peri-tumoral FOP3(+) lymphocyte infiltration, EBV infection, MSI status, Ki-67 intensity, TP53 mutation, HER2 status, LINE-1 methylation level, and CpG methylator phenotype (by using MGMT, CHFR, and CDKN2A). However, there was no significant differences. We could not find any prognostic difference between the two, in disease-specific survival, time to recurrence, relapse-free survival, or overall-survival.

Academic Significance and Societal Importance of the Research Achievements

microbiome は癌、肥満、炎症性腸疾患、非アルコール性脂肪性肝疾患との関連が明らかにされ、注目を集めている研究の一つである。これまで我々は大腸癌患者では腸内細菌叢の中でFusobacterium が健常人よりも多く生息し、Fusobacterium が多い症例では予後が不良であることを解明した。今回、胃腺癌や食道胃接合部腺癌におけるFusobacteriumの有無が、臨床病理的・分子生物学的に、あるいは予後・再発に影響を与えるかについて検討したが、有意な差が得られなかった。上部消化管癌においてFusobacteriumは腫瘍の悪性化に関与していない可能性が高いと考えられた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Recent incidence trend of surgically resected esophagogastric junction adenocarcinoma and microsatellite instability status in Japanese patients.2018

    • Author(s)
      Imamura Y, Watanabe M, Toihata T, Takamatsu M, Kawachi H, Haraguchi I, Ogata Y, Yoshida N, Saeki H, Oki E, Taguchi K, Yamamoto M, Morita M, Mine S, Hiki N, Baba H, Sano T.
    • Journal Title

      Digestion.

      Volume: 99 Issue: 1 Pages: 6-13

    • DOI

      10.1159/000494406

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Progress in characterizing the linkage between Fusobacterium nucleatum and gastrointestinal cancer2018

    • Author(s)
      Liu Yang、Baba Yoshifumi、Ishimoto Takatsugu、Iwatsuki Masaaki、Hiyoshi Yukiharu、Miyamoto Yuji、Yoshida Naoya、Wu Rong、Baba Hideo
    • Journal Title

      Journal of Gastroenterology

      Volume: 54 Issue: 1 Pages: 33-41

    • DOI

      10.1007/s00535-018-1512-9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Review of the gut microbiome and esophageal cancer: Pathogenesis and potential clinical implications.2017

    • Author(s)
      Baba Y, Iwatsuki M, Yoshida N, Watanabe M, Baba H
    • Journal Title

      Ann Gastroenterol Surg

      Volume: 1 Issue: 2 Pages: 99-104

    • DOI

      10.1002/ags3.12014

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fusobacterium nucleatum in gastroenterological cancer: Evaluation of measurement methods using quantitative polymerase chain reaction and a literature review.2017

    • Author(s)
      Yamamura K, Baba Y, Miyake K, Nakamura K, Shigaki H, Mima K, Kurashige J, Ishimoto T, Iwatsuki M, Sakamoto Y, Yamashita Y, Yoshida N, Watanabe M, Baba H
    • Journal Title

      Oncol Lett

      Volume: 14(6) Pages: 6373-6378

    • DOI

      10.3892/ol.2017.7001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The role of intestinal bacteria in the development and progression of gastrointestinal tract neoplasms.2017

    • Author(s)
      Mima K, Ogino S, Nakagawa S, Sawayama H, Kinoshita K, Krashima R, Ishimoto T, Imai K, Iwatsuki M, Hashimoto D, Baba Y, Sakamoto Y, Yamashita YI, Yoshida N, Chikamoto A, Ishiko T, Baba H
    • Journal Title

      Surg Oncol

      Volume: 26(4) Issue: 4 Pages: 368-376

    • DOI

      10.1016/j.suronc.2017.07.011

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The microbiome and hepatobiliary-pancreatic cancers.2017

    • Author(s)
      Mima K, Nakagawa S, Sawayama H, Ishimoto T, Imai K, Iwatsuki M, Hashimoto D, Baba Y, Yamashita YI, Yoshida N, Chikamoto A, Baba H
    • Journal Title

      Cancer Lett.

      Volume: 402 Pages: 9-15

    • DOI

      10.1016/j.canlet.2017.05.001

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human Microbiome Fusobacterium Nucleatum in Esophageal Cancer Tissue Is Associated with Prognosis2016

    • Author(s)
      Yamamura K, Baba Y, Nakagawa S, Mima K, Miyake K, Nakamura K, Sawayama H, Kinoshita K, Ishimoto T, Iwatsuki M, Sakamoto Y, Yamashita Y, Yoshida N, Watanabe M, Baba H.
    • Journal Title

      Clin Cancer Res

      Volume: 22(22) Issue: 22 Pages: 5574-5581

    • DOI

      10.1158/1078-0432.ccr-16-1786

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Microbiome Furobacterium nucleatum has an effect on chemotherapeutic response in esophageal squamous cell carcinoma.2019

    • Author(s)
      Baba Y, Li Y, Eto K, Hiyoshi Y, Ishimoto T, Iwatsuki M, Iwagami S, Miyamoto Y, Yoshida N, Baba H
    • Organizer
      11th AACR-JCA Joint Conference on Breakthroughs in Cancer Research
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 胃癌組織におけるFusobacterium nucleatumの検出と臨床病理学的検討2017

    • Author(s)
      岩槻政晃、織田枝里、馬場祥史、美馬浩介、山村謙介、澤山 浩、坂本快郎、吉田直矢、馬場秀夫
    • Organizer
      第49回胃病態機能研究会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Related Report
      2016 Research-status Report
  • [Presentation] The relationship between the expression of F.nucleatumin gastric cancer and clinical characteristics2017

    • Author(s)
      織田枝里、馬場祥史、美馬浩介、澤山 浩、木下浩一、岩槻政晃、坂本快郎、吉田直矢、馬場秀夫
    • Organizer
      第89回日本胃癌学会総会
    • Place of Presentation
      広島国際会議場(広島県)
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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