| Project/Area Number |
16K10506
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
猪股 雅史 大分大学, 医学部, 教授 (60315330)
平塚 孝宏 大分大学, 医学部, 助教 (20600886)
西園 晃 大分大学, 医学部, 教授 (70218155)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 遺伝子改変型レオウイルス / 光療法 / 5-アミノレブリン酸 / iRFP720遺伝子 / レオウイルス / 光線療法 / 消化管癌 |
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| Outline of Final Research Achievements |
We have successfully made a constructed reovirus, which was transfected iRFP720 gene by reverse genetics method. The visibility of the constructed reovirus in BHK/T7-9 cell line was examined by immunofluorescence microscopy. The constructed reovirus was visualized in this line. In addition, cytopathic effect of constructed reovirus was observed in BHK/T7-9 cell line. The labeled reovirus was visualized in cancer cell line. In future, we are going to examine the cytopathic effect and visualization of the constructed reovirus to cancer cell lines.
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| Academic Significance and Societal Importance of the Research Achievements |
(1) レオウイルスは活性化ras経路を持つ癌特異的にその有効性が期待できること
レオウイルスの最大の特徴は活性化ras経路内で増殖し細胞溶解を起こすことである。膵癌などにおいてレオウイルスの感受性を認めており、多くの癌が標的となる可能性がある。
(2) レオウイルスをベクターとして用いることで、iRFP720を腹膜転移巣およびリンパ節転移巣に特異的に移行させること
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