| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We investigated the roles of Ring finger protein 126 (RNF126) in the progression of gastric cancer. In the immunohistochemical analyses, the RNF126 expression level was significantly associated with the tumor depth. The patients with a RNF126-high tumor had a significantly lower overall survival rate than the patients with a RNF126-low tumor. Multivariate analysis identified the RNF126 status as an independent prognostic factor. We further investigated the roles of RNF126 in vitro using a small interfering RNA (siRNA). RNF126 gene silencing significantly inhibited the proliferation of human gastric cancer cells, MKN45 and MKN74, through the cell cycle arrest. Furthermore, RNF126 gene silencing improved the sensitivity to 5-FU. Our findings indicate that RNF126 might play an important role in regulating the proliferation and chemo-resistance of gastric cancer cells.
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