Detection of Circulating MicroRNAs with Ago2 Complexes to Monitor the Tumor Dynamics of Colorectal Liver Metastasis Patients during Chemotherapy

• Project/Area Number: 16K10573
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Okayama University
• Principal Investigator: Umeda Yuzo 岡山大学, 大学病院, 講師 (10573735)
• Co-Investigator(Kenkyū-buntansha): 藤原 俊義 岡山大学, 医歯薬学総合研究科, 教授 (00304303) ; 八木 孝仁 岡山大学, 大学病院, 教授 (00304353) ; 永坂 岳司 川崎医科大学, 医学部, 准教授 (30452569)
• Research Collaborator: FUJI Tomokazu ; MORI Yoshiko ; YASUI Kazuya
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Precision medicine / 大腸癌肝転移 / Liquid Biopsy / マイクロRNA / Liquid biopsy / Precision Medicine / 遺伝子解析

Outline of Final Research Achievements

We examined Two different forms of circulating miRNAs in plasma: Argonaute2 (Ago2)-miRNAs and extracellular vesicles (EVmiRNAs). Ago2-miR-21 could be released into the extracellular fluid by active export from viable cancer cells and cytolysis in vitro. As miR-21 and miR-200c were expressed in both liver metastasis and primary lesions, we evaluated Ago2-miR-21 as a candidate biomarker of both active export and cytolysis while Ago2-miR-200c as a biomarker of cytolysis in a series of plasma obtained from colorectal cancer (CRC) patients with liver metastasis who received systemic chemotherapy. Ago2-miR-21 allowed us to distinguish CRC from subjects without CRC. The trend in ΔCt values for Ago2-miR-21 and miR-200c during chemotherapy could predict tumor response to ongoing treatment. Thus, capturing circulating Ago2-miRNAs from active export can screen patients with tumor burdens, while capturing them from passive release by cytolysis can monitor tumor dynamics during chemotherapy.

Academic Significance and Societal Importance of the Research Achievements

本研究により、循環Ago2-miRNAを対象としたLiquid Biopsyによる非侵襲的かつ簡便に遺伝子変異情報を把握する新しい臨床検査法が確立され、癌スクリーニングや進行癌におけるダイナミックな腫瘍病勢のモニタリングが可能となった。
本研究手法によって、精緻な個別化医療が叶えば、今後の本邦の大腸癌治療成績を飛躍的に発展させるだけでなく、無用な補助療法を回避する等、患者側面のみならず診療費削減といったCost benefitにも十分に寄与し得る。また同手法は、他の肝癌に対する肝移植や他の消化器悪性疾患の外科治療にも応用できる可能性があり、本臨床医学研究の意義は極めて深いと考えられる。

Research Products

• [Journal Article] Clinical outcomes of women with ovarian metastases of colorectal cancer treated with oophorectomy with respect to their somatic mutation profiles. (2018)
  • Author(s): Mori Y, Nyuya A, Yasui K, Toshima T, Kawai T, Taniguchi F, Kimura K, Inada R, Nishizaki M, Haraga J, Nakamura K, Umeda Y, Kishimoto H, Fujiwara T, Katata Y, Yamaguchi Y, Nagasaka T.
  • Journal Title: Oncotarget Volume: 27;9(23) Issue: 23 Pages: 16477-16488
  • DOI: 10.18632/oncotarget.24735
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prognostic Factors for Pediatric Living Donor Liver Transplantation: Impact of Zero-mortality Transplant for Cholestatic Diseases (2018)
  • Author(s): Morikawa T, Inada R, Nagasaka T, Mori Y, Kishimoto H, Kawai T, Umeda Y, Mishima H, Goel A, Fujiwara T.
  • Journal Title: Acta Medica Okayama Volume: 72 Issue: 6 Pages: 567-576
  • DOI: 10.18926/AMO/56374
  • NAID: 120006545159
  • URL: https://ousar.lib.okayama-u.ac.jp/56374
  • Peer Reviewed / Open Access
• [Journal Article] Long-term Remission of Hepatitis-associated Aplastic Anemia Possibly due to Immunosuppressive Therapy after Liver Transplantation (2018)
  • Author(s): Kanamitsu K, Chayama K, Washio K, Yoshida R, Umeda Y, Yagi T, Shimada A.
  • Journal Title: Acta Medica Okayama Volume: 72 Issue: 5 Pages: 515-518
  • DOI: 10.18926/AMO/56250
  • NAID: 120006532073
  • URL: https://ousar.lib.okayama-u.ac.jp/56250
  • Peer Reviewed
• [Journal Article] Comparison of success rates in eye drop instillation between sitting position and supine position. (2018)
  • Author(s): Naito T, Yoshikawa K, Namiguchi K, Mizoue S, Shiraishi A, Ichikawa Y, Fujiwara M, Miki T, Araki R, Umeda Y, Morizane Y, Shiraga F.
  • Journal Title: PLoS One Volume: 20;13(9) Issue: 9 Pages: e0204363-e0204363
  • DOI: 10.1371/journal.pone.0204363
  • Peer Reviewed
• [Journal Article] The Outcome of Complex Hepato-Pancreato-Biliary Surgery for Elderly Patients: A Propensity Score Matching Analysis. (2018)
  • Author(s): Takagi K, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Fushimi T, Fujiwara T, Yagi T.
  • Journal Title: Dig Surg. Volume: Jun 26 Issue: 4 Pages: 1-8
  • DOI: 10.1159/000489826
  • Peer Reviewed
• [Journal Article] A subclinical high tricuspid regurgitation pressure gradient independent of the mean pulmonary artery pressure is a risk factor for the survival after living donor liver transplantation. (2018)
  • Author(s): Saragai Y, Takaki A, Umeda Y, Matsusaki T, Yasunaka T, Oyama A, Kaku R, Nakamura K, Yoshida R, Nobuoka D, Kuise T, Takagi K, Adachi T, Wada N, Takeuchi Y, Koike K, Ikeda F, Onishi H, Shiraha H, Nakamura S, Morimatsu H, Ito H, Fujiwara T, Yagi T, Okada H.
  • Journal Title: BMC Gastroenterol Volume: 15;18(1) Issue: 1 Pages: 62-62
  • DOI: 10.1186/s12876-018-0793-z
  • Peer Reviewed / Open Access
• [Journal Article] Effect of an enhanced recovery after surgery protocol in patients undergoing pancreaticoduodenectomy: A randomized controlled trial. (2018)
  • Author(s): Takagi K, Yoshida R, Yagi T, Umeda Y, Nobuoka D, Kuise T, Hinotsu S, Matsusaki T, Morimatsu H, Eguchi J, Wada J, Senda M, Fujiwara T.
  • Journal Title: Clinical Nutrition Volume: in press Issue: 1 Pages: 174-181
  • DOI: 10.1016/j.clnu.2018.01.002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of outcomes between symptomatic and asymptomatic patients with colorectal cancer: a propensity score-matched analysis of surgical invasiveness, medical costs and oncological outcomes. (2018)
  • Author(s): Inada R, Nagasaka T, Watanabe A, Yagi T, Mori Y, Kondo Y, Kishimoto H, Umeda Y, Fujiwara T.
  • Journal Title: BMJ Open Gastroenterol. Volume: 3;4(1) Issue: 1 Pages: e000146-e000146
  • DOI: 10.1136/bmjgast-2017-000146
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Living Donor Liver Transplantation for Acute Liver Failure : Comparing Guidelines on the Prediction of Liver Transplantation. (2017)
  • Author(s): Yoshida K, Umeda Y, Takaki A, Nagasaka T, Yoshida R, Nobuoka D, Kuise T, Takagi K, Yasunaka T, Okada H, Yagi T, Fujiwara T.
  • Journal Title: Acta Med Okayama Volume: 71(5) Pages: 381-390
  • DOI: 10.3892/ol.2017.7553
  • NAID: 120006352357
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel intestinal rotation method for digestive reconstruction after combined pancreaticoduodenectomy and extended right hemicolectomy: A case report and surgical technique. (2017)
  • Author(s): Takagi K, Yagi T, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Kumano K, Kojima T, Fushimi T, Fujiwara T.
  • Journal Title: Int J Surg Case Rep Volume: 39 Pages: 51-55
  • DOI: 10.1016/j.ijscr.2017.07.063
  • Peer Reviewed / Open Access
• [Journal Article] Primary pancreatic-type acinar cell carcinoma of the jejunum with tumor thrombus extending into the mesenteric venous system: a case report and literature review. (2017)
  • Author(s): Takagi K, Yagi T, Tanaka T, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Fujiwara T.
  • Journal Title: BMC Surg Volume: 17(1): Issue: 1 Pages: 75-75
  • DOI: 10.1186/s12893-017-0273-3
  • Peer Reviewed / Open Access
• [Journal Article] Radiographic sarcopenia predicts postoperative infectious complications in patients undergoing pancreaticoduodenectomy. (2017)
  • Author(s): Takagi K, Yoshida R, Yagi T, Umeda Y, Nobuoka D, Kuise T, Fujiwara T.
  • Journal Title: BMC Surg Volume: 26;17(1) Issue: 1 Pages: 64-64
  • DOI: 10.1186/s12893-017-0261-7
  • Peer Reviewed / Open Access
• [Journal Article] Preoperative Controlling Nutritional Status (CONUT) Score for Assessment of Prognosis Following Hepatectomy for Hepatocellular Carcinoma. (2017)
  • Author(s): Takagi K, Yagi T, Umeda Y, Shinoura S, Yoshida R, Nobuoka D, Kuise T, Araki H, Fujiwara T.
  • Journal Title: World J Surg. Volume: 41(9) Issue: 9 Pages: 2353-2360
  • DOI: 10.1007/s00268-017-3985-8
  • Peer Reviewed / Open Access
• [Journal Article] Early Chimerism After Liver Transplantation Reflects the Clinical Course of Recurrent Hepatitis C. (2017)
  • Author(s): Utsumi M, Takaki A, Umeda Y, Koike K, Napier SC, Watanabe N, Shinoura S, Yoshida R, Nobuoka D, Yasunaka T, Oto T, Araki M, Yamamoto K, Fujiwara T, Yagi T.
  • Journal Title: Annals of Transplantation. Volume: 24;22 Pages: 156-165
  • Peer Reviewed / Open Access
• [Journal Article] Predictive biomarkers for the efficacy of peptide vaccine treatment: based on the results of a phase II study on advanced pancreatic cancer. (2017)
  • Author(s): Shindo Y, Hazama S, Suzuki N, Iguchi H, Uesugi K, Tanaka H, Aruga A, Hatori T, Ishizaki H, Umeda Y, Fujiwara T, Ikemoto T, Shimada M, Yoshimatsu K, Takenouchi H, Matsui H, Kanekiyo S, Iida M, Koki Y, Arima H, Furukawa H, Ueno T, Yoshino S, Fujita T, Kawakami Y, Nakamura Y, Oka M, Nagano H.
  • Journal Title: J Exp Clin Cancer Res. Volume: 36 Issue: 1 Pages: 36-36
  • DOI: 10.1186/s13046-017-0509-1
  • Peer Reviewed / Open Access
• [Journal Article] Safety and Efficacy of Small Bowel Examination by Capsule Endoscopy for Patients before Liver Transplantation. (2017)
  • Author(s): Seiji K, Akinobu T, Masaya I, Tetsuya Y, Yoshiyasu K, Kou M, Toshihiro I, Yoshiro K, Yuzo U, Takahito Y, Hiroyuki O.
  • Journal Title: Biomed Res Int. Volume: 2017: Pages: 8193821-8193821
  • DOI: 10.1155/2017/8193821
  • Peer Reviewed
• [Journal Article] Syndrome of Inappropriate Antidiuretic Hormone Secretion Following Liver Transplantation (2017)
  • Author(s): Takagi K, Yagi T, Shinoura S, Umeda Y, Yoshida R, Nobuoka D, Watanabe N, Kuise T, Fuji T, Araki H, Fujiwara T.
  • Journal Title: Acta Medica Okayama Volume: 71 Issue: 1 Pages: 85-89
  • DOI: 10.18926/AMO/54830
  • NAID: 120005971560
  • URL: https://ousar.lib.okayama-u.ac.jp/54830
  • Peer Reviewed
• [Journal Article] Phase II clinical trial of peptide cocktail therapy for patients with advanced pancreatic cancer: VENUS-PC study. (2017)
  • Author(s): Suzuki N, Hazama S, Iguchi H, Uesugi K, Tanaka H, Hirakawa K, Aruga A, Hatori T, Ishizaki H, Umeda Y, Fujiwara T, Ikemoto T, Shimada M, Yoshimatsu K, Shimizu R, Hayashi H, Sakata K, Takenouchi H, Matsui H, Shindo Y, Iida M, Koki Y, Arima H, Furukawa H, Ueno T, Yoshino S, Nakamura Y, Oka M, Nagano H.
  • Journal Title: Cancer Sci. Volume: 108(1) Issue: 1 Pages: 73-80
  • DOI: 10.1111/cas.13113
  • Peer Reviewed / Open Access
• [Journal Article] Expansion of epigenetic alterations in EFEMP1 promoter predicts malignant formation in pancreatobiliary intraductal papillary mucinous neoplasms. (2016)
  • Author(s): Yoshida K, Nagasaka T, Umeda Y, Tanaka T, Kimura K, Taniguchi F, Fuji T, Shigeyasu K, Mori Y, Yanai H, Yagi T, Goel A, Fujiwara T.
  • Journal Title: J Cancer Res Clin Oncol. Volume: - Issue: 7 Pages: 1-13
  • DOI: 10.1007/s00432-016-2164-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tumor-specific delivery of biologics by a novel T-cell line HOZOT. (2016)
  • Author(s): Onishi T, Tazawa H, Hashimoto Y, Takeuchi M, Otani T, Nakamura S, Sakurai F, Mizuguchi H, Kishimoto H, Umeda Y, Shirakawa Y, Urata Y, Kagawa S, Fujiwara T.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 38060-38060
  • DOI: 10.1038/srep38060
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Improved Resection and Outcome of Colon-Cancer Liver Metastasis with Fluorescence-Guided Surgery Using In Situ GFP Labeling with a Telomerase-Dependent Adenovirus in an Orthotopic Mouse Model (2016)
  • Author(s): Yano S, Takehara K, Miwa S, Kishimoto H, Hiroshima Y, Murakami T, Urata Y, Kagawa S, Bouvet M, Fujiwara T, Hoffman RM
  • Journal Title: PLoS One Volume: 11 Issue: 2 Pages: e0148760-e0148760
  • DOI: 10.1371/journal.pone.0148760
  • Open Access / Int'l Joint Research
• [Journal Article] Surgical Outcome of Patients Undergoing Pancreaticoduodenectomy: Analysis of a 17 Year Experience at a Single Center (2016)
  • Author(s): Takagi K, Yagi T, Yoshida R, Shinoura S, Umeda Y, Nobuoka D, Kuise T, Watanabe N, Sui K, Fuji T, Fujiwara T
  • Journal Title: Acta Medica Okayama Volume: 70 Pages: 197-203
  • NAID: 120005770688
  • Peer Reviewed
• [Journal Article] Comparison of in vitro invasiveness of high- and low-metastatic triple-negative human breast cancer visualized by color-coded imaging (2016)
  • Author(s): Yano S, Takehara K, Tazawa H, Kishimoto H, Kagawa S, Fujiwara T, Hoffman RM
  • Journal Title: In Vitro Cell Dev Biol Anim Volume: 53 Issue: 2 Pages: 96-98
  • DOI: 10.1007/s11626-016-0092-3
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Sarcopenia and American Society of Anesthesiologists Physical Status in the Assessment of Outcomes of Hepatocellular Carcinoma Patients Undergoing Hepatectomy (2016)
  • Author(s): Takagi K, Yagi T, Yoshida R, Shinoura S, Umeda Y, Nobuoka D, Kuise T, Watanabe N, Fujiwara T
  • Journal Title: Acta Medica Okayama Volume: 70 Pages: 363-370
  • NAID: 120005859864
  • Peer Reviewed
• [Presentation] Liquid Biopsy has a potential to predict the colorectal cancer patients with destiny for recurrence after curative surgery (2018)
  • Author(s): Toshima T, Nyuya A, Umeda Y, Yasui K, Yoshida K, Fujiwara T, Goel A, Nagasaka T
  • Organizer: ESMO Asia 2018, Singapore
  • Int'l Joint Research
• [Presentation] Prediction of salvage liver transplantation for hepatocellular carcinoma recurrence: when and for which patient should we decide transplant? (2018)
  • Author(s): Umeda Y, Yagi T, Yoshida R, Nobuoka D, Kuise T, Yoshida K, Yasui K, Shirakawa Y, Fujiwara T
  • Organizer: 第80回日本臨床外科学会総会
• [Presentation] De novo Malignancies after Liver Transplantation and a proposal for establishment of cancer screening (2018)
  • Author(s): Yoshida K, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Yasui K, Takaki A, Fujiwara T, Yagi T
  • Organizer: Asian Transplantation Week 2018, Busan, Korea
  • Int'l Joint Research
• [Presentation] 大腸癌における新規 oncogenic small nucleolar RNA (oncSNOR) の探求 (2018)
  • Author(s): 吉田一博, 重安邦俊, 楳田祐三, 吉田龍一, 信岡大輔, 杭瀬 崇, 安井和也, 香川俊輔, 白川靖博, 八木孝仁, Goel A, 藤原俊義
  • Organizer: 第29回日本消化器癌発生学会総会
• [Presentation] 大腸癌多発肝転移に対する治療戦略 -遺伝子変異解析を踏まえた術前化学療法の適応選別- (2018)
  • Author(s): 楳田祐三, 八木孝仁, 吉田龍一, 信岡大輔, 杭瀬 崇, 吉田一博, 安井和也, 香川俊輔, 白川靖博, 藤原俊義
  • Organizer: 第73回日本消化器外科学会総会
• [Presentation] 大腸癌多発肝転移に対する肝切除～多回肝切除と化学療法を見据えたParenchymal sparing ＆ skeletonized hepatectomy～ (2018)
  • Author(s): 楳田祐三, 八木孝仁, 永坂岳司, 吉田龍一, 信岡大輔, 杭瀬 崇, 伏見卓郎, 香川俊輔, 白川靖博, 藤原俊義
  • Organizer: 第118回日本外科学会定期学術集会
• [Presentation] Minimum requirement of Pancreatoduodenectomy for certified expert HBP surgeon: surgical outcome of 387 cases and the learning curve analysis (2018)
  • Author(s): Yoshida K, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Yasui K, Konishi D, Okabayashi H, Yagi T, Fujiwara T
  • Organizer: 第30回日本肝胆膵外科学会学術集会
• [Presentation] 肝切除における血清アルブミン動態 -アルブミン製剤至適投与に向けて臨床指標を再考する- (2018)
  • Author(s): 吉田一博, 楳田祐三, 吉田龍一, 信岡大輔, 杭瀬 崇, 安井和也, 香川俊輔, 白川靖博, 八木孝仁, 藤原俊義
  • Organizer: 第73回日本消化器外科学会総会
• [Presentation] Gene expression profiles in BRAF V600E mutant colorectal cancer and association with SFRP2 methylation status (2017)
  • Author(s): Yasui K, Nagasaka T, ToshimaT, Kawai T, Shigeyasu K, Mori Y, Haraga J, Nakamura K, Umeda Y, Tazawa H, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2017, Washington, D.C.
  • Int'l Joint Research
• [Presentation] 遺伝子変異情報に基づいた大腸癌肝転移の治療戦略―原発巣の左右局在とRAS/RAF 変異の意義― (2017)
  • Author(s): 楳田祐三, 永坂岳司, 母里淑子, 吉田龍一, 信岡大輔, 杭瀬 崇, 藤 智和, 河合 毅, 荒木宏之, 八木孝仁, 藤原俊義
  • Organizer: 第117回日本外科学会定期学術集会, 横浜
• [Presentation] StageIV大腸癌に対するPrecision Medicineの構築 (2017)
  • Author(s): 河合 毅, 永坂岳司, 母里淑子, 楳田祐三, 稲田 涼, 谷口文崇, 安井和也, 戸嶋俊明, 岸本浩行, 藤原俊義
  • Organizer: 第117回日本外科学会定期学術集会, 横浜
• [Presentation] Radical distal pancreatectomy with en block celiac axis resection and retroperitoneal dissection for advanced pancreatic cancer (2017)
  • Author(s): Umeda Y, Yagi T, Yoshida R, Nobuoka D, Kuise T, Koujima T, Kumano K, Takagi K, Araki H, Kajioka H, Fujiwara T
  • Organizer: Joint Congress of The 6th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association & The 29th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery, Yokohama
  • Int'l Joint Research
• [Presentation] Clinical significance of radical lymphadenectomy for intrahepatic cholangiocarcinoma - analysis based on tumor location and recurrence pattern - (2017)
  • Author(s): Kuise T, Umeda Y, Yoshida R, Nobuoka D, Kumano K, Koujima T, Takagi K, Araki H, Kajioka H, Fujiwara T, Yagi T
  • Organizer: Joint Congress of The 6th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association & The 29th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery, Yokohama
  • Int'l Joint Research
• [Presentation] Hepatectomy for multiple colorectal liver metastasis - precise tumor removal and parenchymal sparing - (2017)
  • Author(s): Kajioka H, Umeda Y, Yoshida R, Nagasaka T, Nobuoka D, Kuise T, Koujima T, Kumano K, Araki H, Fujiwara T, Yagi T
  • Organizer: Joint Congress of The 6th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association & The 29th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery, Yokohama
  • Int'l Joint Research
• [Presentation] Surgical approach to cirrhotic patients with hepatocellular carcinoma: splenectomy ameliorates liver function which could expand the chance of hepatectomy (2017)
  • Author(s): Koujima T, Umeda Y, Watanabe N, Yoshida R, Nobuoka D, Kuise T, Kumano K, Takagi K, Araki H, Fujiwara T, Yagi T
  • Organizer: Joint Congress of The 6th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association & The 29th Meeting of Japanese Society of Hepato-Biliary-Pancreatic Surgery, Yokohama
  • Int'l Joint Research
• [Presentation] Ovarian metastasectomy in colorectal cancer may improve the clinical outcomes of patients with metastatic colorectal cancer regardless of BRAF or KRAS mutational profiles (2017)
  • Author(s): Mori Y, Nagasaka T, Tanioka H, Nyuya A, Kawai T, Toshima T, Yasui K, Shigeyasu K, Kishimoto H, Umeda Y, Fujiwara T
  • Organizer: ESMO 19th World Congress on Gastrointestinal Cancer, Barcelona, Spain
  • Int'l Joint Research
• [Presentation] 大腸癌多発肝転移におけるRAS/RAF変異の意義～生物学的悪性度から術前化学療法適応を見極める～ (2017)
  • Author(s): 楳田祐三, 八木孝仁, 永坂岳司, 吉田龍一, 信岡大輔, 母里淑子, 杭瀬 崇, 高木弘誠, 河合 毅, 藤原俊義
  • Organizer: 第72回日本消化器外科学会総会, 金沢
• [Presentation] 局所進行直腸癌に対する術前化学療法の有用性 (2017)
  • Author(s): 母里淑子, 永坂岳司, 河合 毅, 重安邦俊, 矢野修也, 近藤喜太, 楳田祐三, 岸本浩行, 藤原俊義
  • Organizer: 第72回日本消化器外科学会総会, 金沢
• [Presentation] Gene expression signatures in BRAF V600E mutant colorectal cancer in relation to WNT signaling cascade (2017)
  • Author(s): Yasui K, Nagasaka T, Nyuya A, Toshima T, Kawai T, Shigeyasu K, Yano S, Mori Y, Haraga J, Nakamura K, Umeda Y, Goel A, Fujiwara T
  • Organizer: ESMO 2017 Congress, Madrid
  • Int'l Joint Research
• [Presentation] Heterogenity of epigenetic and EMT marks observed in hepatocellular carcinoma with keratin 19 proficiency (2017)
  • Author(s): Nagasaka T, Okada T, Yokomichi N, Taniguchi F, Toshima T, Yasui K, Fuji T, Mori Y, Kawai T, Umeda Y, Yagi T, Fujiwara T, Goel A
  • Organizer: ESMO 2017 Congress, Madrid
  • Int'l Joint Research
• [Presentation] A novel circulating cell free DNA-based assay can predict tumor response to systematic chemotherapy (2017)
  • Author(s): Toshima T, Nagasaka T, Mori Y, Kawai T, Yasui K, Fuji T, Taniguchi F, Kimura K, Kishimoto H, Umeda Y, Goel A, Fujiwara T
  • Organizer: ESMO 2017 Congress, Madrid
  • Int'l Joint Research
• [Presentation] Prediction of salvage liver transplantation for hcc recurrence: when and which patient should we decide transplant? (2017)
  • Author(s): Umeda Y, Yagi T, Yoshida R, Nobuoka D, Kuise T, Kumano K, Koujima T, Takagi K, Nagasaka T, Fujiwara T
  • Organizer: 18th Congress of the European Society for Organ Transplantation, Barcelona
  • Int'l Joint Research
• [Presentation] Is poor outcome of living donor liver transplantation for primary sclerosing cholangitis the nature of the disease itself or insufficient immunosuppression? (2017)
  • Author(s): Yagi T, Nobuoka D, Umeda Y, Yoshida R, Kuise T, Takagi K, Kumano K, Fujiwara T
  • Organizer: 18th Congress of the European Society for Organ Transplantation, Barcelona
  • Int'l Joint Research
• [Presentation] Surgical outcomes of living donor liver surgery: technical knack for zero morbidity (2017)
  • Author(s): Kuise T, Umeda Y, Yoshida R, Nobuoka D, Kumano K, Koujima T, Takagi K, Fujiwara T, Yagi T
  • Organizer: 18th Congress of the European Society for Organ Transplantation, Barcelona
  • Int'l Joint Research
• [Presentation] 切除不能進行再発大腸がんの２次治療以降における Ramcirumab + FOLFIRI 療法の治療成績 (2017)
  • Author(s): 永坂岳司, 母里淑子, 重安邦俊, 河合 毅, 矢野修也, 岸本浩行, 楳田祐三, 山口佳之, 藤原俊義
  • Organizer: 第55回日本癌治療学会学術集会, 横浜
• [Presentation] 安全で正確な系統的肝切除の実践に向けて：Knack & Pitfalls (2017)
  • Author(s): 楳田祐三, 八木孝仁, 吉田龍一, 信岡大輔, 杭瀬 崇, 國府島 健, 伏見卓郎, 香川俊輔, 白川靖博, 藤原俊義
  • Organizer: 第79回日本臨床外科学会総会, 東京
  • Invited
• [Presentation] Preoperative proximal splenic artery embolization: alternative portal modulation for prophylaxis of small-for-size syndrome (2017)
  • Author(s): Umeda Y, Yagi T, Yoshida R, Nobuoka D, Kuise T, Kumano K, Koujima T, Takagi K, Nagasaka T, Fujiwara T
  • Organizer: Asian Transplantation Week 2017 (ATW 2017), Incheon
  • Int'l Joint Research
• [Presentation] Prediction of salvage liver transplantation for hepatocellular carcinoma recurrence: when and for which patient should we decide transplant? (2017)
  • Author(s): Fushimi T, Umeda Y, Yoshida R, Nobuoka D, Kuise T, Takagi K, Yasunaka T, Takaki A, Fujiwara T, Yagi T
  • Organizer: Asian Transplantation Week 2017 (ATW 2017), Incheon
  • Int'l Joint Research
• [Presentation] Efficacious use of Tolvaptan for refractory ascites and hyponatremia in perioperative management in liver transplantation (2017)
  • Author(s): Kuise T, Umeda Y, Yoshida R, Nobuoka D, Kumano K, Koujima T, Takagi K, Fujiwara T, Yagi T
  • Organizer: Asian Transplantation Week 2017 (ATW 2017), Incheon
  • Int'l Joint Research
• [Presentation] テロメラーゼ標的蛍光発現ウィルスを用いた腹腔内遊離膵癌細胞診断技術の開発 (2017)
  • Author(s): 吉田龍一, 桒田和也, 楳田祐三, 信岡大輔, 杭瀬 崇, 國府島 健, 熊野健二郎, 伏見卓郎, 香川俊輔, 八木孝仁, 藤原俊義
  • Organizer: 第44回日本膵切研究会
• [Presentation] 安全で正確な肝切除：ICG 蛍光法によるfusion imagingとSoft凝固付CUSA による肝静脈露出 (2016)
  • Author(s): 楳田祐三, 八木孝仁, 篠浦 先, 吉田龍一, 信岡大輔, 渡辺信之, 杭瀬 崇, 藤 智和, 高木弘誠, 荒木宏之, 藤原俊義
  • Organizer: 第78回日本臨床外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2016-11-26
• [Presentation] 肝硬変合併肝細胞癌に対する脾摘の肝機能改善効果 (2016)
  • Author(s): 渡辺信之, 楳田祐三, 篠浦 先, 吉田龍一, 信岡大輔, 杭瀬 崇, 藤 智和, 高木弘誠, 荒木宏之, 八木孝仁, 藤原俊義
  • Organizer: 第78回日本臨床外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2016-11-24
• [Presentation] The Utility of Cadaver-Based Approaches for the Surgical Training in Hepatobiliary and Pancreatic Surgery (2016)
  • Author(s): Nobuoka D, Yagi T, Kondo Y, Yoshida R, Umeda Y, Shinoura S, Takeda Y, Ohtsuka A, Fujiwara T
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: Kyoto
  • Year and Date: 2016-10-25
  • Int'l Joint Research
• [Presentation] Risk Factors for Recurrence in the Remnant Pancreas after Partial Pancreatectomy for Intraductal Papillary Mucinous Neoplasms (IPMNs) (2016)
  • Author(s): Yoshida R, Fuji T, Shinoura S, Umeda Y, Nobuoka D, Watanabe N, Kuise T, Takagi K, Araki H, Yagi T, Fujiwara T
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: Kyoto
  • Year and Date: 2016-10-25
  • Int'l Joint Research
• [Presentation] Prediction of salvage liver transplantation for hepatocellular carcinoma recurrence:until when are we tenacious of locoregional therapy? (2016)
  • Author(s): Umeda Y, Yagi T, Nagasaka T, Shinoura S, Yoshida R, Nobuoka D, Watanabe N, Kuise T, Fuji T, Takagi K, Fujiwara T
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: Kyoto
  • Year and Date: 2016-10-24
  • Int'l Joint Research
• [Presentation] IDH1/2 およびKRAS 遺伝子の変異ステータスによる肝内胆管癌の層別化 (2016)
  • Author(s): 安井和也, 永坂岳司, 楳田祐三, 藤智和, 戸嶋俊明, 河合毅, 母里淑子, 八木孝仁, 藤原俊義
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-06
• [Presentation] Surgical technique for minimal invasiveness in living donor liver surgery: Knack & Pitfalls (2016)
  • Author(s): Umeda Y, Yagi T, Shinoura S, Yoshida R, Nobuoka D, Watanabe N, Kuise T, Fuji T, Takagi K, Fujiwara T.
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-16
• [Presentation] Acquired Resistance to Anti-Epidermal Growth Factor Receptor Therapy in Metastatic Colorectal Cancer (2016)
  • Author(s): 谷口文崇, 永坂岳司, 藤 智和, 母里淑子, 岸本浩行, 河合 毅, 楳田祐三, 藤原俊義
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-15
• [Presentation] Lynch症候群および家族性大腸腺腫症の系統的遺伝子解析 (2016)
  • Author(s): 谷口文崇, 永坂岳司, 楳田祐三, 母里淑子, 河合 毅, 内海方嗣, 田中屋宏爾, 青木秀樹, 竹内仁司, 藤原俊義
  • Organizer: 第85回大腸癌研究会
  • Place of Presentation: 大阪
  • Year and Date: 2016-07-01
• [Presentation] 再肝切除における根治性確保と合併症軽減に向けた手術手技と工夫 (2016)
  • Author(s): 楳田祐三, 八木孝仁, 篠浦 先, 吉田龍一, 信岡大輔, 渡辺信之, 杭瀬 崇, 藤 智和, 高木弘誠, 藤原俊義
  • Organizer: 第28回日本肝胆膵外科学会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-06-02
• [Presentation] MGMT Inactivation Arrest Tumor Growth and Serves As a Promising Predictive Biomarker for Treatment Response in Colorectal Cancer (2016)
  • Author(s): Mori Y, Nagasaka T, Tazawa H, Umeda Y, Shigeyasu K, Yoshida K, Goel A, Fujiwara T
  • Organizer: Digestive Disease Week (DDW) 2016
  • Place of Presentation: San Diego
  • Year and Date: 2016-05-24
  • Int'l Joint Research
• [Presentation] 肝内胆管癌に対するリンパ節郭清の意義 (2016)
  • Author(s): 楳田祐三, 篠浦 先, 吉田龍一, 信岡大輔, 渡辺信之, 杭瀬 崇, 藤 智和, 高木弘誠, 八木孝仁, 藤原俊義
  • Organizer: 第70回手術手技研究会
  • Place of Presentation: 東京
  • Year and Date: 2016-05-21
• [Presentation] Comprehensive analysis of intrahepatic cholangiocarcinoma based on viral infections and mutational status in the IDH1/2 and KRAS genes (2016)
  • Author(s): Yasui K, Nagasaka T, Umeda Y, Fuji T, Taniguchi F, Toshima T, Kimura K, Kawai T, Mori Y, Tazawa H, Yagi T, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-19
  • Int'l Joint Research
• [Presentation] 膵癌切除症例における免疫チェックポイント分子の発現解析～膵癌切除例とTCGA dataを対象とした免疫チェックポイント機構の解析～ (2016)
  • Author(s): 藤 智和, 楳田祐三, 永坂岳司, 母里淑子, 谷口文崇, 安井和也, 河合 毅, 八木孝仁, 藤原俊義
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-16
• [Presentation] 肝内胆管癌の外科治療～リンパ節郭清結果を踏まえ手術戦略を再考する～ (2016)
  • Author(s): 渡辺信之, 楳田祐三, 篠浦 先, 吉田龍一, 信岡大輔, 杭瀬 崇, 須井健太, 藤 智和, 高木弘誠, 木村裕司, 八木孝仁, 藤原俊義
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-16
• [Presentation] Efficacy of enhanced alcoholism treatment program before a liver transplantation for patients with alcoholic liver cirrhosis (2016)
  • Author(s): Yoshida R, Takaki A, Matsumoto Y, Yasuda Y, Takagi K, Sui K, Fuji T, Kuise T, Watanabe N, Nobuoka D, Umeda Y, Shinoura S, Yagi T
  • Organizer: TSS Asian Regional Meeting 2016
  • Place of Presentation: Tokyo
  • Year and Date: 2016-04-09
  • Int'l Joint Research
• [Presentation] Efficacy of Tolvaptan for refractory ascites and hyponatremia in perioperative management in liver transplantation (2016)
  • Author(s): Umeda Y, Yagi T, Takaki A, Shinoura S, Yoshida R, Nobuoka D, Yasunaka T, Watanabe N, Kuise T, Fuji T, Sui K, Takagi K, Nagasaka T, Fujiwara T
  • Organizer: TSS Asian Regional Meeting 2016
  • Place of Presentation: Tokyo
  • Year and Date: 2016-04-08
  • Int'l Joint Research